2020
Horizontally acquired regulatory gene activates ancestral regulatory system to promote Salmonella virulence
Choi J, Groisman EA. Horizontally acquired regulatory gene activates ancestral regulatory system to promote Salmonella virulence. Nucleic Acids Research 2020, 48: 10832-10847. PMID: 33045730, PMCID: PMC7641745, DOI: 10.1093/nar/gkaa813.Peer-Reviewed Original ResearchConceptsPhoP/PhoQAncestral regulatorNucleoid structuring protein H-NSProtein H-NSS. bongoriSalmonella enterica serovar TyphimuriumAncestral genomesH-NSTarget promotersEnterica serovar TyphimuriumRegulatory genesBacterial virulenceSalmonella virulencePromoter regionSsrBOpposite regulationGenesS. typhimuriumPhoQRegulatory systemSerovar TyphimuriumVirulenceTranscriptionPromoterRegulator
2013
The lipopolysaccharide modification regulator PmrA limits Salmonella virulence by repressing the type three-secretion system Spi/Ssa
Choi J, Groisman EA. The lipopolysaccharide modification regulator PmrA limits Salmonella virulence by repressing the type three-secretion system Spi/Ssa. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 9499-9504. PMID: 23690578, PMCID: PMC3677452, DOI: 10.1073/pnas.1303420110.Peer-Reviewed Original ResearchConceptsPmrA proteinNull mutantsSalmonella virulenceThree secretion systemVirulence regulatory genesSalmonella enterica serovar TyphimuriumWild-type SalmonellaEnterica serovar TyphimuriumPmrA mutantRegulatory genesProtein bindsModification genesMurine typhoid feverControl expressionLPS modificationsPmrA geneMutantsGenesSerovar TyphimuriumPathogen persistenceAntimicrobial peptidesHost tissuesPromoterVirulenceProtein
2010
An antisense RNA that governs the expression kinetics of a multifunctional virulence gene
Lee EJ, Groisman EA. An antisense RNA that governs the expression kinetics of a multifunctional virulence gene. Molecular Microbiology 2010, 76: 1020-1033. PMID: 20398218, PMCID: PMC2909850, DOI: 10.1111/j.1365-2958.2010.07161.x.Peer-Reviewed Original ResearchConceptsAntisense RNAGenome-wide transcriptome analysisRegulatory protein PhoPLong antisense RNAProtein levelsAntisense transcriptionMgtC proteinTranscriptome analysisRNase EAntisense transcriptsPolycistronic messageRNase IIISalmonella virulenceBacterial speciesPhysiological roleExpression kineticsTranscriptionRNAVirulence genesApparent roleVirulencePhoPSalmonella entericaMgtCAmgRS
2005
Virulence and drug resistance roles of multidrug efflux systems of Salmonella enterica serovar Typhimurium
Nishino K, Latifi T, Groisman EA. Virulence and drug resistance roles of multidrug efflux systems of Salmonella enterica serovar Typhimurium. Molecular Microbiology 2005, 59: 126-141. PMID: 16359323, DOI: 10.1111/j.1365-2958.2005.04940.x.Peer-Reviewed Original ResearchConceptsDrug efflux systemsEfflux systemSalmonella virulenceEscherichia coli homologueToxic compound extrusionResponse regulator PhoPATP-binding cassette (ABC) superfamiliesPhoP/PhoQOuter membrane componentsAmino acid sequenceDrug resistance rolesTwo-component systemSalmonella enterica serovar TyphimuriumGram-negative speciesMultidrug efflux systemsSame operonEnterica serovar TyphimuriumRegulator PhoPCompound extrusionAcid sequenceMajor facilitatorEfflux system genesPromoter regionVirulence phenotypesMajor regulatorThe PhoP/PhoQ system controls the intramacrophage type three secretion system of Salmonella enterica
Bijlsma JJ, Groisman EA. The PhoP/PhoQ system controls the intramacrophage type three secretion system of Salmonella enterica. Molecular Microbiology 2005, 57: 85-96. PMID: 15948951, DOI: 10.1111/j.1365-2958.2005.04668.x.Peer-Reviewed Original ResearchConceptsSPI-2 pathogenicity islandPhoP/PhoQ systemType three secretion systemThree secretion systemPhoP proteinSecretion systemSsa genesPathogenicity islandTwo-component regulatory systemResponse regulator SsrBPhoP/PhoQRegulatory systemTwo-component systemSalmonella entericaEukaryotic cellsChromatin immunoprecipitationCentral roleDifferent regulatory systemsSalmonella virulenceUntranslated regionDistinct signalsPhoPGenesEffectorsProtein
2004
Activation of the RcsC/YojN/RcsB phosphorelay system attenuates Salmonella virulence
Mouslim C, Delgado M, Groisman EA. Activation of the RcsC/YojN/RcsB phosphorelay system attenuates Salmonella virulence. Molecular Microbiology 2004, 54: 386-395. PMID: 15469511, DOI: 10.1111/j.1365-2958.2004.04293.x.Peer-Reviewed Original ResearchConceptsConstitutive mutantsSalmonella virulenceNon-phagocytic cellsPhosphorelay systemWild-type SalmonellaExpression of productsVirulence functionsFull virulenceSensor geneCps operonRcsBConstitutive activationSuccessful infectionVirulence attenuationGenesVirulence factorsAberrant expressionBacterial pathogensVirulenceMutantsHost tissuesExpressionLocation-dependent mannerMutationsAttenuation phenotype
1999
Pathogenicity Islands and the Evolution of Salmonella Virulence
Groisman E, Blanc‐Potard A, Uchiya K. Pathogenicity Islands and the Evolution of Salmonella Virulence. 1999, 127-150. DOI: 10.1128/9781555818173.ch7.Peer-Reviewed Original ResearchSPI-1 genesPathogenicity islandDNA segmentsSPI-1Evolution of SalmonellaCultured epithelial cellsSalmonella-specific sequenceTRNA genesExport apparatusTranscriptional unitsLarge operonNonhost environmentsSecretion systemBacterial entryHousekeeping genesSalmonella virulenceHost rangeSPI-2Secretory systemHost cellsAspects of infectionNonphagocytic cellsGenesMacrophage apoptosisOperon
1997
The Salmonella selC locus contains a pathogenicity island mediating intramacrophage survival
Blanc‐Potard A, Groisman E. The Salmonella selC locus contains a pathogenicity island mediating intramacrophage survival. The EMBO Journal 1997, 16: 5376-5385. PMID: 9311997, PMCID: PMC1170169, DOI: 10.1093/emboj/16.17.5376.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAnimalsBacterial ProteinsBase SequenceCarrier ProteinsCation Transport ProteinsCulture MediaEscherichia coliEscherichia coli ProteinsFemaleGenes, BacterialMacrophagesMagnesiumMembrane Transport ProteinsMiceMice, Inbred BALB CMolecular Sequence DataMultigene FamilyMutationOperonPhenotypeRecombination, GeneticRNA, TransferSalmonella Infections, AnimalSalmonella typhimuriumConceptsPathogenicity islandSelC locusIntramacrophage survivalSalmonella typhimurium chromosomePhoP/PhoQKb DNA segmentVirulence genesNon-pathogenic bacterial speciesTwo-component systemSite of integrationChromosomal clustersTRNA locusVirulence functionsDNA segmentsSalmonella virulenceHomologous regionsMajor regulatorBacterial speciesLociEnteric bacteriaEscherichia coliGenesBenign strainsLow Mg2Virulence
1996
Mg2+ as an Extracellular Signal: Environmental Regulation of Salmonella Virulence
Véscovi E, Soncini F, Groisman E. Mg2+ as an Extracellular Signal: Environmental Regulation of Salmonella Virulence. Cell 1996, 84: 165-174. PMID: 8548821, DOI: 10.1016/s0092-8674(00)81003-x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBacterial ProteinsBase SequenceCationsGene Expression Regulation, BacterialHydrogen-Ion ConcentrationKineticsMagnesiumMolecular Sequence DataMutationPeptidesPhenotypeProtein ConformationSalmonella typhimuriumSensitivity and SpecificitySignal TransductionTranscription FactorsVirulenceConceptsPhoP/PhoQ systemTranscription of PhoPSignal transduction cascadeVirulence regulatory systemGene expression patternsPeriplasmic domainWild-type SalmonellaExtracellular signalsTransduction cascadeSalmonella virulenceExpression patternsFirst messengersPhoPRegulatory systemGenesPhoQSalmonella typhimuriumPhysiological concentrationsDivalent cationsTranscriptionConcentration of Mg2LociMg2DomainVirulence
1995
Regulation of Salmonella Virulence by Two‐Component Regulatory Systems
Groisman E, Heffron F. Regulation of Salmonella Virulence by Two‐Component Regulatory Systems. 1995, 319-332. DOI: 10.1128/9781555818319.ch20.Peer-Reviewed Original ResearchTwo-component systemEnvZ/OmpR systemTwo-component regulatory systemClassical bacterial geneticsIdentification of lociPhoP/PhoQCrucial virulence factorTranscription regulatory factorsOuter membrane porins OmpCTissue culture model systemTranscriptional regulationBacterial geneticsVirulence defectBacterial pathogenesisMicrobial pathogenesisEnvironmental cuesCulture model systemSalmonella virulenceMutant strainRegulatory factorsPorins OmpCS. typhimuriumRegulatory systemVirulence determinantsAdaptive response
1994
The role of the PhoP/PhoQ regulon in Salmonella virulence
Véscovi E, Soncini FC, Groisman EA. The role of the PhoP/PhoQ regulon in Salmonella virulence. Research In Microbiology 1994, 145: 473-480. PMID: 7855434, DOI: 10.1016/0923-2508(94)90096-5.Peer-Reviewed Original Research
1992
Resistance to host antimicrobial peptides is necessary for Salmonella virulence.
Groisman EA, Parra-Lopez C, Salcedo M, Lipps CJ, Heffron F. Resistance to host antimicrobial peptides is necessary for Salmonella virulence. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 11939-11943. PMID: 1465423, PMCID: PMC50673, DOI: 10.1073/pnas.89.24.11939.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsAntimicrobial Cationic PeptidesBiological EvolutionBlood ProteinsChromosome MappingCytoplasmic GranulesDefensinsGenes, BacterialGenotypeGranulocytesHumansIn Vitro TechniquesMagaininsMiceMutagenesisPeptidesProtaminesSalmonella InfectionsSalmonella typhimuriumXenopus ProteinsConceptsDifferent phenotypic classesTransposon insertion mutantsHost defense strategiesSalmonella virulence genesBacterium Salmonella typhimuriumResistance mechanismsAntimicrobial peptidesDefensin NP-1Antimicrobial peptide protamineInsertion mutantsResistance lociHost antimicrobial peptidesSalmonella virulencePhenotypic classesMutantsSuccessful pathogenDefective lipopolysaccharideAntibacterial peptidesVirulence genesPeptide mastoparanCecropin P1Defense strategiesUbiquitous typeVirulence propertiesGenes