2023
Everglades virus: an underrecognized disease-causing subtype of Venezuelan equine encephalitis virus endemic to Florida, USA
Burkett-Cadena N, Fish D, Weaver S, Vittor A. Everglades virus: an underrecognized disease-causing subtype of Venezuelan equine encephalitis virus endemic to Florida, USA. Journal Of Medical Entomology 2023, 60: 1149-1164. PMID: 37862065, PMCID: PMC10645373, DOI: 10.1093/jme/tjad070.Peer-Reviewed Original ResearchConceptsEverglades virusEncephalitis virusCases of meningitisCotton rat Sigmodon hispidusHigh natural infection rateEpidemic strainsNatural infection rateInfection rateWidespread transmissionAbundance of AedesNatural infectionSubtype IISubtypesFrequent feedingFrequency of contactCertain mosquitoesVector competenceVirusHuman diseasesRodentsVertebrate hostsA ticking time bomb hidden in plain sight
Narasimhan S, Fish D, Pedra J, Pal U, Fikrig E. A ticking time bomb hidden in plain sight. Science Translational Medicine 2023, 15: eadi7829. PMID: 37851823, DOI: 10.1126/scitranslmed.adi7829.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus Statements
2022
Seasonal Dynamics of Mosquito-Borne Viruses in the Southwestern Florida Everglades, 2016, 2017.
Anderson J, Fish D, Armstrong P, Misencik M, Bransfield A, Ferrandino F, Andreadis T, Stenglein M, Kapuscinski M. Seasonal Dynamics of Mosquito-Borne Viruses in the Southwestern Florida Everglades, 2016, 2017. American Journal Of Tropical Medicine And Hygiene 2022, 106: 610-622. PMID: 35008051, PMCID: PMC8832897, DOI: 10.4269/ajtmh.20-1547.Peer-Reviewed Original ResearchConceptsSpecies of virusesSpecies of mosquitoesGumbo LimboShotgun metagenomic sequencingShark RiverSpecies complexWest Nile virusHardwood hammocksEverglades virusNotable speciesMosquito-Borne VirusesMetagenomic sequencingAnopheles speciesNile virusSeasonal dynamicsSpeciesFlorida EvergladesTensaw virusCulex nigripalpusEvergladesPools of CxCypress swampsNigripalpusMosquitoesAnopheles crucians
2021
Emergence potential of mosquito-borne arboviruses from the Florida Everglades
Fish D, Tesh R, Guzman H, da Rosa A, Balta V, Underwood J, Sither C, Vasilakis N. Emergence potential of mosquito-borne arboviruses from the Florida Everglades. PLOS ONE 2021, 16: e0259419. PMID: 34807932, PMCID: PMC8608345, DOI: 10.1371/journal.pone.0259419.Peer-Reviewed Original ResearchConceptsHardwood hammocksEverglades regionWater levelEnvironmental changesTensaw virusBig Cypress National PreserveLargest natural wetlandWetland restoration projectsGreater Everglades regionEverglades National ParkMajor landscape featuresMajor environmental changesEmergence of arbovirusesInvasive speciesUndergoing restorationNatural wetlandsRestoration projectsSubtropical ecosystemsFlorida EvergladesLandscape featuresState ForestNational ParkNational PreservePathogenic mosquitoEverglades virus
2018
Borrelia miyamotoi: An Emerging Tick-Borne Pathogen
Wormser GP, Shapiro ED, Fish D. Borrelia miyamotoi: An Emerging Tick-Borne Pathogen. The American Journal Of Medicine 2018, 132: 136-137. PMID: 30144406, DOI: 10.1016/j.amjmed.2018.08.012.Peer-Reviewed Original ResearchResponse to “Transfusion‐transmitted and community‐acquired babesiosis in New York, 2004 to 2015: a response to why and what to do”
Fish D, Krause PJ. Response to “Transfusion‐transmitted and community‐acquired babesiosis in New York, 2004 to 2015: a response to why and what to do”. Transfusion 2018, 58: 1818-1819. PMID: 30133825, DOI: 10.1111/trf.14761.Peer-Reviewed Original ResearchConceptsResponseCommunity‐acquired and transfusion‐transmitted babesiosis are increasing: why and what to do?
Kumar S, Fish D, Krause PJ. Community‐acquired and transfusion‐transmitted babesiosis are increasing: why and what to do? Transfusion 2018, 58: 617-619. PMID: 29528151, DOI: 10.1111/trf.14518.Peer-Reviewed Original Research
2017
Human rickettsial pathogen modulates arthropod organic anion transporting polypeptide and tryptophan pathway for its survival in ticks
Taank V, Dutta S, Dasgupta A, Steeves TK, Fish D, Anderson JF, Sultana H, Neelakanta G. Human rickettsial pathogen modulates arthropod organic anion transporting polypeptide and tryptophan pathway for its survival in ticks. Scientific Reports 2017, 7: 13256. PMID: 29038575, PMCID: PMC5643405, DOI: 10.1038/s41598-017-13559-x.Peer-Reviewed Original ResearchCharacterization of Three New Insect-Specific Flaviviruses: Their Relationship to the Mosquito-Borne Flavivirus Pathogens
Guzman H, Contreras-Gutierrez MA, da Rosa A, Nunes MRT, Cardoso JF, Popov VL, Young KI, Savit C, Wood TG, Widen SG, Watts DM, Hanley KA, Perera D, Fish D, Vasilakis N, Tesh RB. Characterization of Three New Insect-Specific Flaviviruses: Their Relationship to the Mosquito-Borne Flavivirus Pathogens. American Journal Of Tropical Medicine And Hygiene 2017, 98: 410-419. PMID: 29016330, PMCID: PMC5929187, DOI: 10.4269/ajtmh.17-0350.Peer-Reviewed Original ResearchLyme disease ecology in a changing world: consensus, uncertainty and critical gaps for improving control
Kilpatrick AM, Dobson ADM, Levi T, Salkeld DJ, Swei A, Ginsberg HS, Kjemtrup A, Padgett KA, Jensen PM, Fish D, Ogden NH, Diuk-Wasser MA. Lyme disease ecology in a changing world: consensus, uncertainty and critical gaps for improving control. Philosophical Transactions Of The Royal Society B Biological Sciences 2017, 372: 20160117. PMID: 28438910, PMCID: PMC5413869, DOI: 10.1098/rstb.2016.0117.Peer-Reviewed Original ResearchConceptsNorth AmericaLyme disease ecologyDeer abundanceDifferent habitatsVector-borne zoonotic diseaseTemperate regionsDisease ecologyLyme disease preventionHost speciesDisease riskTick abundanceKnowledge gapsTemporal variationReservoir competenceCommon tick-borne diseaseEcologySubstantial uncertaintyAbundanceHost communitiesTick-borne diseaseTick populationsCritical gapAmericaAreas of consensusDisease preventionTicks elicit variable fibrinogenolytic activities upon feeding on hosts with different immune backgrounds
Vora A, Taank V, Dutta SM, Anderson JF, Fish D, Sonenshine DE, Catravas JD, Sultana H, Neelakanta G. Ticks elicit variable fibrinogenolytic activities upon feeding on hosts with different immune backgrounds. Scientific Reports 2017, 7: 44593. PMID: 28300174, PMCID: PMC5353578, DOI: 10.1038/srep44593.Peer-Reviewed Original ResearchConceptsDifferent immune backgroundsImmune backgroundImmunodeficient animalsImmunocompetent animalsAge-matched miceAntibody-blocking assaysIxodes scapularis ticksFibrinogenolytic activityD-dimerAnti-hemostatic factorsImmune defenseTick feedingScapularis ticksReduced levelsBlood feedingEngorgement weightLC-MS/MSFibrinogenolysisNovel roleAnimalsPCR analysisTicksFeedingGenetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus
Nunes MRT, Contreras-Gutierrez MA, Guzman H, Martins LC, Barbirato MF, Savit C, Balta V, Uribe S, Vivero R, Suaza JD, Oliveira H, Neto J, Carvalho VL, da Silva SP, Cardoso JF, de Oliveira RS, da Silva Lemos P, Wood TG, Widen SG, Vasconcelos PFC, Fish D, Vasilakis N, Tesh RB. Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus. Virology 2017, 504: 152-167. PMID: 28193550, PMCID: PMC5394984, DOI: 10.1016/j.virol.2017.01.022.Peer-Reviewed Original ResearchConceptsInsect-specific virusesGenetic characterizationNovel insect-specific virusesPlant virus generaMajor phylogenetic groupsHigh genetic variabilityComprehensive genetic characterizationFull-length sequencesCross-species transmissionPhylogenetic relationshipsEvolutionary analysisVirus taxaGenetic variabilityPhylogenetic groupsDistinct generaVirus genusNegevirusesMolecular analysisGenusMolecular epidemiologyDiverse groupPhlebotomine sandfliesTaxaArthropodsVirusClosely-related Borrelia burgdorferi (sensu stricto) strains exhibit similar fitness in single infections and asymmetric competition in multiple infections
Rynkiewicz EC, Brown J, Tufts DM, Huang CI, Kampen H, Bent SJ, Fish D, Diuk-Wasser MA. Closely-related Borrelia burgdorferi (sensu stricto) strains exhibit similar fitness in single infections and asymmetric competition in multiple infections. Parasites & Vectors 2017, 10: 64. PMID: 28166814, PMCID: PMC5292797, DOI: 10.1186/s13071-016-1964-9.Peer-Reviewed Original ResearchConceptsAsymmetric competitive interactionsPatterns of coexistenceBiotic contextCommon disease vectorPathogen communitiesVector-borne pathogensBorrelia burgdorferiLong-term persistencePathogen speciesAsymmetric competitionPathogen dynamicsInfection phenotypesSimilar fitnessTransmission phenotypeDisease vectorsTemperate regionsCompetitive interactionsTransmission advantagePathogen strainsPathogen persistenceBlack-legged tickCompetitive strainsPeromyscus leucopusWhite-footed miceHostAssessment of synthetic floral-based attractants and sugar baits to capture male and female Aedes aegypti (Diptera: Culicidae)
Fikrig K, Johnson BJ, Fish D, Ritchie SA. Assessment of synthetic floral-based attractants and sugar baits to capture male and female Aedes aegypti (Diptera: Culicidae). Parasites & Vectors 2017, 10: 32. PMID: 28095875, PMCID: PMC5240245, DOI: 10.1186/s13071-016-1946-y.Peer-Reviewed Original ResearchConceptsGravid Aedes TrapFemale AeSugar baitsFemale Aedes aegyptiATSB solutionsZika virusAedes aegyptiConclusionsOur findingsNumber of mosquitoesNulliparous femalesPublic healthMalesControl programsSugar feedingVirusMale AeAegyptiFemalesAttraction assaysMango nectarAssaysPhysiological needsControlGravid female Ae
2016
Almendravirus: A Proposed New Genus of Rhabdoviruses Isolated from Mosquitoes in Tropical Regions of the Americas.
Contreras MA, Eastwood G, Guzman H, Popov V, Savit C, Uribe S, Kramer LD, Wood TG, Widen SG, Fish D, Tesh RB, Vasilakis N, Walker PJ. Almendravirus: A Proposed New Genus of Rhabdoviruses Isolated from Mosquitoes in Tropical Regions of the Americas. American Journal Of Tropical Medicine And Hygiene 2016, 96: 100-109. PMID: 27799634, PMCID: PMC5239673, DOI: 10.4269/ajtmh.16-0403.Peer-Reviewed Original ResearchConceptsL protein sequencesSimilar genome organizationStructural protein genesAnimal rhabdovirusesGenome organizationUnique cladePhylogenetic analysisEverglades National ParkArthropod populationsProtein geneProtein sequencesL geneVertebrate hostsNew genusSequence analysisDiverse familyHematophagous arthropodsRNA virusesAnopheles quadrimaculatus mosquitoesGenesNational ParkCladeArthropodsNovel virusRhabdovirusA Cost-Effectiveness Tool for Informing Policies on Zika Virus Control
Alfaro-Murillo JA, Parpia AS, Fitzpatrick MC, Tamagnan JA, Medlock J, Ndeffo-Mbah ML, Fish D, Ávila-Agüero ML, Marín R, Ko AI, Galvani AP. A Cost-Effectiveness Tool for Informing Policies on Zika Virus Control. PLOS Neglected Tropical Diseases 2016, 10: e0004743. PMID: 27205899, PMCID: PMC4874682, DOI: 10.1371/journal.pntd.0004743.Peer-Reviewed Original ResearchConceptsGuillain-Barré syndromeZika infectionHealth burdenVirus controlRisk of microcephalyDirect medical costsCost-effectiveness toolsCurrent Zika outbreakGBS casesMedical costsZika casesZika virusMicrocephalyIncidence dataCountry of implementationZika outbreakInfectionInterventionBurdenInforming PolicyDALYsCountry-specific settingsOutbreakMosquitoesPregnancyEvaluating the effectiveness of localized control strategies to curtail chikungunya
Ndeffo-Mbah ML, Durham DP, Skrip LA, Nsoesie EO, Brownstein JS, Fish D, Galvani AP. Evaluating the effectiveness of localized control strategies to curtail chikungunya. Scientific Reports 2016, 6: 23997. PMID: 27045523, PMCID: PMC4820747, DOI: 10.1038/srep23997.Peer-Reviewed Original ResearchConceptsAcute febrile phaseChronic joint painRe-emerging arbovirusOutbreak of chikungunyaJoint painFebrile phaseSymptomatic casesChikungunya casesChikungunya outbreakTropical diseasesDiseaseAlbopictus mosquitoesDisease surveillanceChikungunyaTransmission dynamicsPrimary measureVector control effortsAedes aegyptiMosquito movementContainment strategiesOutbreakSubsequent spreadVector controlMosquitoesPain
2015
Response to Esteve-Gassent et al.: flaB sequences obtained from Texas PCR products are identical to the positive control strain Borrelia burgdorferi B31
Norris SJ, Barbour AG, Fish D, Diuk-Wasser MA. Response to Esteve-Gassent et al.: flaB sequences obtained from Texas PCR products are identical to the positive control strain Borrelia burgdorferi B31. Parasites & Vectors 2015, 8: 310. PMID: 26050617, PMCID: PMC4489397, DOI: 10.1186/s13071-015-0899-x.Peer-Reviewed Original ResearchAssociation between body size and reservoir competence of mammals bearing Borrelia burgdorferi at an endemic site in the northeastern United States
Barbour AG, Bunikis J, Fish D, Hanincová K. Association between body size and reservoir competence of mammals bearing Borrelia burgdorferi at an endemic site in the northeastern United States. Parasites & Vectors 2015, 8: 299. PMID: 26024881, PMCID: PMC4459683, DOI: 10.1186/s13071-015-0903-5.Peer-Reviewed Original ResearchConceptsBody sizeLarge-sized mammalsMedium-sized mammalsDifferent mammalian speciesEastern North AmericaSpecies-specific PCRLyme disease agentSmall mammalsMammalian speciesWhite-footed mouseCommon raccoonSized mammalsMammalsBorrelia burgdorferiPine volesBody massGrey squirrelsVirginia opossumReservoir competenceEastern chipmunksIxodes scapularis ticksDisease agentsStriped skunksSpeciesNymphal Ixodes scapularis ticksBorrelia miyamotoi infection in nature and in humans
Krause PJ, Fish D, Narasimhan S, Barbour AG. Borrelia miyamotoi infection in nature and in humans. Clinical Microbiology And Infection 2015, 21: 631-639. PMID: 25700888, PMCID: PMC4470780, DOI: 10.1016/j.cmi.2015.02.006.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsB. miyamotoi infectionMiyamotoi infectionClinical manifestationsB. miyamotoiLyme diseaseBorrelia miyamotoi infectionAcute febrile illnessCommon clinical manifestationsBlood smear examinationPublic health importanceHuman granulocytic anaplasmosisFever group spirochetesFebrile illnessAntibiotic therapyDisease groupEtiologic diagnosisSevere diseaseSmear examinationIxodes persulcatus ticksHealth importanceInfectionHuman casesGranulocytic anaplasmosisBorrelia miyamotoiDisease