2020
Extracorporeal Photochemotherapy: Mechanistic Insights Driving Recent Advances and Future Directions.
Wei BM, Hanlon D, Khalil D, Han P, Tatsuno K, Sobolev O, Edelson RL. Extracorporeal Photochemotherapy: Mechanistic Insights Driving Recent Advances and Future Directions. The Yale Journal Of Biology And Medicine 2020, 93: 145-159. PMID: 32226344, PMCID: PMC7087063.Peer-Reviewed Original ResearchConceptsDendritic cellsExtracorporeal photopheresisDC maturationProfessional antigen-presenting cellsAntigen-specific immunityAntigen-presenting cellsImmunological capabilitiesAutoimmune disordersCancer immunotherapyLymphoid tissueImmune systemImmunotherapyInfectious diseasesPoor survivabilityTherapeutic endsPoor availabilityMaturationMultiple strategiesPhotopheresisCellsCancerPhotochemotherapyDiseaseImmunity
2019
Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy
Hanlon D, Sobolev O, Han P, Ventura A, Vassall A, Kibbi N, Yurter A, Robinson E, Filler R, Tatsuno K, Edelson RL. Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy. Methods In Molecular Biology 2019, 2097: 173-195. PMID: 31776926, DOI: 10.1007/978-1-0716-0203-4_11.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsAntigen-presenting cellsDendritic cellsTumor-associated antigensBlood monocytesDC populationsExtracorporeal photochemotherapyCytokine-derived DCsSource of DCDendritic antigen-presenting cellsHuman peripheral blood mononuclear cellsFunctional antigen-presenting cellsVivo T cell stimulationBlood mononuclear cellsMurine bone marrow precursorsApoptotic tumor cellsT cell stimulationBone marrow precursorsCancer immunotherapyDC differentiationMononuclear cellsIL-4Clinical vaccinationMonocyte activationSupraphysiologic concentrations
2010
Polymer nanoparticles containing tumor lysates as antigen delivery vehicles for dendritic cell–based antitumor immunotherapy
Prasad S, Cody V, Saucier-Sawyer JK, Saltzman WM, Sasaki CT, Edelson RL, Birchall MA, Hanlon DJ. Polymer nanoparticles containing tumor lysates as antigen delivery vehicles for dendritic cell–based antitumor immunotherapy. Nanomedicine Nanotechnology Biology And Medicine 2010, 7: 1-10. PMID: 20692374, PMCID: PMC3073408, DOI: 10.1016/j.nano.2010.07.002.Peer-Reviewed Original ResearchConceptsTumor-associated antigensDendritic cellsTumor lysateAntigen presentationT cellsAntigen deliveryNeck squamous carcinoma cell linesPoly (lactic-co-glycolic acid) (PLGA) NPsAnti-tumor CD8Solid organ malignanciesIL-10 productionT cell responsesAnti-tumor vaccinesSquamous carcinoma cell linesNeck cancer cell linesAntigen delivery vehiclesCell linesAdvanced HNSCCAutologous CD8Cancer cell linesOrgan malignanciesAntitumor immunotherapyCarcinoma cell linesImmune mechanismsMonocyte precursors
2007
Polymer Nanoparticles for Immunotherapy from Encapsulated Tumor-Associated Antigens and Whole Tumor Cells
Solbrig CM, Saucier-Sawyer JK, Cody V, Saltzman WM, Hanlon DJ. Polymer Nanoparticles for Immunotherapy from Encapsulated Tumor-Associated Antigens and Whole Tumor Cells. Molecular Pharmaceutics 2007, 4: 47-57. PMID: 17217312, DOI: 10.1021/mp060107e.Peer-Reviewed Original ResearchConceptsPolymer nanoparticlesMixture of proteinsMolecular weight polymersRate of nanoparticlesProtein loadingEfficiency of encapsulationWeight polymersDissolved nanoparticlesNanoparticle formulationDifferent particle loadingsRelease propertiesNanoparticlesPreparation methodEncapsulation efficiencyComplex mixturesMolecular weightSDS-acrylamide gel electrophoresisSolvent extractionProtein assaysAgEncapsulationParticle loadingMixtureSpectrum of proteinsPromising approach
2002
Transimmunization, a novel approach for tumor immunotherapy
Berger CL, Hanlon D, Kanada D, Girardi M, Edelson RL. Transimmunization, a novel approach for tumor immunotherapy. Transfusion And Apheresis Science 2002, 26: 205-216. PMID: 12126207, DOI: 10.1016/s1473-0502(02)00014-9.Peer-Reviewed Original ResearchMeSH KeywordsDendritic CellsHumansImmunotherapyLeukapheresisLymphoma, T-Cell, CutaneousNeoplasmsPhotopheresisConceptsCutaneous T-cell lymphomaImmune responseTumor antigensPotent antigen presenting cellsAnti-tumor immune responseExtracorporeal photopheresis (ECP) treatmentDendritic cell immunotherapyT-cell lymphomaAntigen presenting cellsPeripheral blood monocytesApoptotic tumor cellsApoptotic CTCL cellsPhotopheresis treatmentRejection episodesHost diseaseOvernight incubation periodTumor epitopesImmature DCsCell immunotherapyTumor immunotherapyAutoimmune diseasesDC differentiationInflammatory cytokinesPresenting cellsCell lymphoma