2022
Rapid Screen for Antiviral T‐Cell Immunity with Nanowire Electrochemical Biosensors
Nami M, Han P, Hanlon D, Tatsuno K, Wei B, Sobolev O, Pitruzzello M, Vassall A, Yosinski S, Edelson R, Reed M. Rapid Screen for Antiviral T‐Cell Immunity with Nanowire Electrochemical Biosensors. Advanced Materials 2022, 34: e2109661. PMID: 35165959, DOI: 10.1002/adma.202109661.Peer-Reviewed Original ResearchConceptsT cell immunitySARS-CoV-2Immune responseHuman T cell immune responseAntiviral T cell immunityPathogen-specific T cellsT cell immune responsesT cell analysisPatient's immune responseT cell responsesAntibody-based protectionPandemic SARS-CoV-2Protective immunityT cellsVaccine formulationsB cellsVaccine designBroad protectionDisease riskInfectious diseasesCare toolsTranslational platformImmunityEmergent variantsPandemic coronavirus
2020
Extracorporeal Photochemotherapy: Mechanistic Insights Driving Recent Advances and Future Directions.
Wei BM, Hanlon D, Khalil D, Han P, Tatsuno K, Sobolev O, Edelson RL. Extracorporeal Photochemotherapy: Mechanistic Insights Driving Recent Advances and Future Directions. The Yale Journal Of Biology And Medicine 2020, 93: 145-159. PMID: 32226344, PMCID: PMC7087063.Peer-Reviewed Original ResearchConceptsDendritic cellsExtracorporeal photopheresisDC maturationProfessional antigen-presenting cellsAntigen-specific immunityAntigen-presenting cellsImmunological capabilitiesAutoimmune disordersCancer immunotherapyLymphoid tissueImmune systemImmunotherapyInfectious diseasesPoor survivabilityTherapeutic endsPoor availabilityMaturationMultiple strategiesPhotopheresisCellsCancerPhotochemotherapyDiseaseImmunity
2019
Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy
Hanlon D, Sobolev O, Han P, Ventura A, Vassall A, Kibbi N, Yurter A, Robinson E, Filler R, Tatsuno K, Edelson RL. Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy. Methods In Molecular Biology 2019, 2097: 173-195. PMID: 31776926, DOI: 10.1007/978-1-0716-0203-4_11.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsAntigen-presenting cellsDendritic cellsTumor-associated antigensBlood monocytesDC populationsExtracorporeal photochemotherapyCytokine-derived DCsSource of DCDendritic antigen-presenting cellsHuman peripheral blood mononuclear cellsFunctional antigen-presenting cellsVivo T cell stimulationBlood mononuclear cellsMurine bone marrow precursorsApoptotic tumor cellsT cell stimulationBone marrow precursorsCancer immunotherapyDC differentiationMononuclear cellsIL-4Clinical vaccinationMonocyte activationSupraphysiologic concentrationsEx vivo dendritic cell generation—A critical comparison of current approaches
Han P, Hanlon D, Sobolev O, Chaudhury R, Edelson RL. Ex vivo dendritic cell generation—A critical comparison of current approaches. International Review Of Cytology 2019, 349: 251-307. PMID: 31759433, DOI: 10.1016/bs.ircmb.2019.10.003.Peer-Reviewed Original ResearchConceptsDendritic cellsDiscovery of DCsMemory T cell responsesProfessional antigen-presenting cellsAntigen-specific immune responsesDendritic cell generationAntigen-specific immunityT cell responsesAntigen-presenting cellsEx vivo productionMononuclear cell fractionRalph SteinmanDC therapyAutoimmune disordersImmunologic functionDC functionPoor survivalImmunologic roleImmune responsePeripheral tissuesPhysiologic productionAdaptive immunityClinical utilityTherapeutic modulationImmune systemExtracorporeal photochemotherapy induces bona fide immunogenic cell death
Tatsuno K, Yamazaki T, Hanlon D, Han P, Robinson E, Sobolev O, Yurter A, Rivera-Molina F, Arshad N, Edelson RL, Galluzzi L. Extracorporeal photochemotherapy induces bona fide immunogenic cell death. Cell Death & Disease 2019, 10: 578. PMID: 31371700, PMCID: PMC6675789, DOI: 10.1038/s41419-019-1819-3.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsAntigens, NeoplasmApoptosisCD8-Positive T-LymphocytesCell DifferentiationCell Line, TumorCell SurvivalDendritic CellsHMGB1 ProteinHumansImmunogenic Cell DeathLeukocytesLymphoma, T-Cell, CutaneousMethoxsalenMiceMonocytesPhotopheresisPhotosensitizing AgentsReceptor, Interferon alpha-betaUltraviolet RaysConceptsHigh mobility group box 1Tumor-associated antigensCutaneous T-cell lymphomaWhite blood cellsDendritic cellsImmunostimulatory signalsI interferonBona fide immunogenic cell deathMobility group box 1Such dendritic cellsSyngeneic immunocompetent miceCancer cellsT-cell lymphomaType I IFN receptorGroup box 1Immunogenic cell deathI IFN receptorATP-degrading enzymeSecretion of ATPMelanoma cell viabilityCognate immunityUVA irradiationAnticancer immunityImmunocompetent miceCalreticulin exposureNovel Protocol for Generating Physiologic Immunogenic Dendritic Cells.
Ventura A, Vassall A, Yurter A, Robinson E, Filler R, Hanlon D, Meeth K, Ezaldein H, Girardi M, Sobolev O, Bosenberg MW, Edelson RL. Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells. Journal Of Visualized Experiments 2019 PMID: 31157760, DOI: 10.3791/59370.Peer-Reviewed Original ResearchConceptsCutaneous T-cell lymphomaDendritic cellsCellular vaccinesClinical efficacyAnti-tumor T cell immunityVivo anti-tumor responsesMonocyte-derived dendritic cellsTumor cellsSyngeneic mouse tumor modelsImmunogenic dendritic cellsAnti-cancer immunityT cell immunityAnti-tumor responseHuman dendritic cellsT-cell lymphomaAnti-tumor effectsKey mechanistic driversApoptotic tumor cellsMouse tumor modelsCell immunitySafety profileCancer immunotherapyCell lymphomaMouse modelBlood samples
2015
Configuration-dependent Presentation of Multivalent IL-15:IL-15Rα Enhances the Antigen-specific T Cell Response and Anti-tumor Immunity*
Hong E, Usiskin IM, Bergamaschi C, Hanlon DJ, Edelson RL, Justesen S, Pavlakis GN, Flavell RA, Fahmy TM. Configuration-dependent Presentation of Multivalent IL-15:IL-15Rα Enhances the Antigen-specific T Cell Response and Anti-tumor Immunity*. Journal Of Biological Chemistry 2015, 291: 8931-8950. PMID: 26719339, PMCID: PMC4861462, DOI: 10.1074/jbc.m115.695304.Peer-Reviewed Original ResearchConceptsT cell responsesArtificial antigen-presenting cellsDendritic cellsIL-15Antigen-presenting cellsIL-15RαCell responsesAntigen-specific T cell responsesAntigen-processing dendritic cellsMaximal T cell responsesAnti-tumor immunitySame dendritic cellOptimal immune responseIL-15 functionsMechanism of actionIL-2Antigen deliveryImmune responseDC surfaceParacrine fashionTumor progressionMurine melanomaCellular mechanismsAggressive modelEnhanced potency
2014
Targeting human dendritic cells via DEC-205 using PLGA nanoparticles leads to enhanced cross-presentation of a melanoma-associated antigen
Saluja SS, Hanlon DJ, Sharp FA, Hong E, Khalil D, Robinson E, Tigelaar R, Fahmy TM, Edelson RL. Targeting human dendritic cells via DEC-205 using PLGA nanoparticles leads to enhanced cross-presentation of a melanoma-associated antigen. International Journal Of Nanomedicine 2014, Volume 9: 5231-5246. PMID: 25419128, PMCID: PMC4235494, DOI: 10.2147/ijn.s66639.Peer-Reviewed Original Research
2011
Enhanced Stimulation of Anti‐Ovarian Cancer CD8+ T Cells by Dendritic Cells Loaded with Nanoparticle Encapsulated Tumor Antigen
Hanlon DJ, Aldo PB, Devine L, Alvero AB, Engberg AK, Edelson R, Mor G. Enhanced Stimulation of Anti‐Ovarian Cancer CD8+ T Cells by Dendritic Cells Loaded with Nanoparticle Encapsulated Tumor Antigen. American Journal Of Reproductive Immunology 2011, 65: 597-609. PMID: 21241402, PMCID: PMC3082607, DOI: 10.1111/j.1600-0897.2010.00968.x.Peer-Reviewed Original ResearchMeSH KeywordsAntigen PresentationAntigens, DifferentiationAntigens, NeoplasmCarcinomaCD8-Positive T-LymphocytesCells, CulturedCytokinesDendritic CellsFemaleHumansImmunotherapy, AdoptiveLactic AcidLymphocyte ActivationNanoparticlesOvarian NeoplasmsPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerConceptsTumor-associated antigensT cell responsesT cellsDendritic cellsCytokine productionTumor antigensAnti-tumor T cell responsesCell surface co-stimulatory moleculesCD8 T cell responsesSurface co-stimulatory moleculesAnti-tumor immune responseAntigen-loaded DCsTumor lysate antigensCD8 T cellsCo-stimulatory moleculesT cell expressionHuman DCsActivation markersTumor lysateImmune responseLysate antigenBlood monocytesClinical testingEnhanced stimulationAntigen
2010
Polymer nanoparticles containing tumor lysates as antigen delivery vehicles for dendritic cell–based antitumor immunotherapy
Prasad S, Cody V, Saucier-Sawyer JK, Saltzman WM, Sasaki CT, Edelson RL, Birchall MA, Hanlon DJ. Polymer nanoparticles containing tumor lysates as antigen delivery vehicles for dendritic cell–based antitumor immunotherapy. Nanomedicine Nanotechnology Biology And Medicine 2010, 7: 1-10. PMID: 20692374, PMCID: PMC3073408, DOI: 10.1016/j.nano.2010.07.002.Peer-Reviewed Original ResearchConceptsTumor-associated antigensDendritic cellsTumor lysateAntigen presentationT cellsAntigen deliveryNeck squamous carcinoma cell linesPoly (lactic-co-glycolic acid) (PLGA) NPsAnti-tumor CD8Solid organ malignanciesIL-10 productionT cell responsesAnti-tumor vaccinesSquamous carcinoma cell linesNeck cancer cell linesAntigen delivery vehiclesCell linesAdvanced HNSCCAutologous CD8Cancer cell linesOrgan malignanciesAntitumor immunotherapyCarcinoma cell linesImmune mechanismsMonocyte precursors
2005
Enhanced and prolonged cross‐presentation following endosomal escape of exogenous antigens encapsulated in biodegradable nanoparticles
Shen H, Ackerman AL, Cody V, Giodini A, Hinson ER, Cresswell P, Edelson RL, Saltzman WM, Hanlon DJ. Enhanced and prolonged cross‐presentation following endosomal escape of exogenous antigens encapsulated in biodegradable nanoparticles. Immunology 2005, 117: 78-88. PMID: 16423043, PMCID: PMC1782199, DOI: 10.1111/j.1365-2567.2005.02268.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen PresentationB-LymphocytesBiocompatible MaterialsBiodegradation, EnvironmentalCell LineCross-PrimingDendritic CellsEndosomesHistocompatibility Antigens Class IIHumansLactic AcidLymphocyte ActivationMiceMice, Inbred C57BLNanostructuresOvalbuminPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerPolymersSerum Albumin, BovineT-LymphocytesConceptsBone marrow-derived dendritic cellsMHC class I presentationAntigen-presenting cellsClass I presentationMHC class IExogenous antigensDendritic cellsClass IAntigen deliveryPrimary mouse bone marrow-derived dendritic cellsSoluble antigenMouse bone marrow-derived dendritic cellsMarrow-derived dendritic cellsProfessional antigen-presenting cellsMajor histocompatibility complex class IProtein-based vaccinationT cell responsesClassic MHC class IExogenous antigen presentationHistocompatibility complex class IAntigen-coated latex beadsCell-associated antigensInterleukin-2 secretionComplex class IEfficiency of presentation
2002
Transimmunization, a novel approach for tumor immunotherapy
Berger CL, Hanlon D, Kanada D, Girardi M, Edelson RL. Transimmunization, a novel approach for tumor immunotherapy. Transfusion And Apheresis Science 2002, 26: 205-216. PMID: 12126207, DOI: 10.1016/s1473-0502(02)00014-9.Peer-Reviewed Original ResearchConceptsCutaneous T-cell lymphomaImmune responseTumor antigensPotent antigen presenting cellsAnti-tumor immune responseExtracorporeal photopheresis (ECP) treatmentDendritic cell immunotherapyT-cell lymphomaAntigen presenting cellsPeripheral blood monocytesApoptotic tumor cellsApoptotic CTCL cellsPhotopheresis treatmentRejection episodesHost diseaseOvernight incubation periodTumor epitopesImmature DCsCell immunotherapyTumor immunotherapyAutoimmune diseasesDC differentiationInflammatory cytokinesPresenting cellsCell lymphomaEfficient Tumor Antigen Loading of Dendritic Antigen Presenting Cells by Transimmunization
Girardi M, Berger C, Hanlon D, Edelson RL. Efficient Tumor Antigen Loading of Dendritic Antigen Presenting Cells by Transimmunization. Technology In Cancer Research & Treatment 2002, 1: 65-69. PMID: 12614179, DOI: 10.1177/153303460200100109.Peer-Reviewed Original ResearchConceptsCutaneous T-cell lymphomaMalignant T cellsT cellsTumor antigen loadingAnti-tumor immunityManagement of patientsT-cell lymphomaAntigen presenting cellsDendritic cell differentiationDendritic cellsSelective immunotherapyPresenting cellsOnly FDATumor antigensCell lymphomaAntigen loadingTumor cellsKey cellular eventsTransimmunizationImmunotherapyPatientsCancerRecent findingsEfficient phagocytosisCells