2020
Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model
Tatsuno K, Han P, Edelson R, Hanlon D. Detection of Immunogenic Cell Death in Tumor Vaccination Mouse Model. Methods In Molecular Biology 2020, 2255: 171-186. PMID: 34033103, DOI: 10.1007/978-1-0716-1162-3_15.Peer-Reviewed Original ResearchConceptsImmunogenic cell deathTumor cellsImmune responseHigh mobility box 1Antitumor immune responseCell deathType I IFNVaccination assayTLR agonistsVivo vaccinationICD inducersICD inductionMouse modelI IFNBox 1Animal modelsGold standard methodPreparation of vaccinesMolecular patternsRegulated cell deathHallmark featureDeathIntracellular chaperoneCellsSurface translocationPlatelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes
Han P, Hanlon D, Arshad N, Lee JS, Tatsuno K, Yurter A, Robinson E, Filler R, Sobolev O, Cote C, Rivera-Molina F, Toomre D, Fahmy T, Edelson R. Platelet P-selectin initiates cross-presentation and dendritic cell differentiation in blood monocytes. Science Advances 2020, 6: eaaz1580. PMID: 32195350, PMCID: PMC7065880, DOI: 10.1126/sciadv.aaz1580.Peer-Reviewed Original ResearchConceptsDendritic cellsDifferentiation of monocytesBlood monocytesTumor-specific T cell immunityCytokine-derived DCsT cell immunityAntigen-specific immunityPlatelet P-selectinDendritic cell differentiationPeripheral blood monocytesCell immunityP-selectin glycoprotein ligand-1P-selectinExogenous cytokinesNuclear factorMonocytesPhysiologic maturationPhysiological mannerCalcium fluxingNuclear localizationLigand 1Cell differentiationImmunityRapid maturationPlateletsTransimmunization restores immune surveillance and prevents recurrence in a syngeneic mouse model of ovarian cancer
Alvero AB, Hanlon D, Pitruzzello M, Filler R, Robinson E, Sobolev O, Tedja R, Ventura A, Bosenberg M, Han P, Edelson RL, Mor G. Transimmunization restores immune surveillance and prevents recurrence in a syngeneic mouse model of ovarian cancer. OncoImmunology 2020, 9: 1758869. PMID: 32566387, PMCID: PMC7302442, DOI: 10.1080/2162402x.2020.1758869.Peer-Reviewed Original ResearchConceptsHigh-grade serous ovarian cancerSyngeneic mouse modelOvarian cancerRecurrent diseaseMouse modelRecurrent high-grade serous ovarian cancerEffective anti-tumor immune responseDendritic cell vaccination strategiesHuman cutaneous T cell lymphomaAnti-tumor immune responseMyeloid-derived suppressive cellsCutaneous T-cell lymphomaIntra-peritoneal tumorsWhole tumor antigenChemotherapy-resistant diseaseFirst-line standardT-cell lymphomaOvarian cancer accountsSerous ovarian cancerTumor-associated macrophagesImmunotherapeutic interventionsGynecologic malignanciesSuppressive cellsDisease coursePatient survival
2019
Extracorporeal photochemotherapy induces bona fide immunogenic cell death
Tatsuno K, Yamazaki T, Hanlon D, Han P, Robinson E, Sobolev O, Yurter A, Rivera-Molina F, Arshad N, Edelson RL, Galluzzi L. Extracorporeal photochemotherapy induces bona fide immunogenic cell death. Cell Death & Disease 2019, 10: 578. PMID: 31371700, PMCID: PMC6675789, DOI: 10.1038/s41419-019-1819-3.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsAntigens, NeoplasmApoptosisCD8-Positive T-LymphocytesCell DifferentiationCell Line, TumorCell SurvivalDendritic CellsHMGB1 ProteinHumansImmunogenic Cell DeathLeukocytesLymphoma, T-Cell, CutaneousMethoxsalenMiceMonocytesPhotopheresisPhotosensitizing AgentsReceptor, Interferon alpha-betaUltraviolet RaysConceptsHigh mobility group box 1Tumor-associated antigensCutaneous T-cell lymphomaWhite blood cellsDendritic cellsImmunostimulatory signalsI interferonBona fide immunogenic cell deathMobility group box 1Such dendritic cellsSyngeneic immunocompetent miceCancer cellsT-cell lymphomaType I IFN receptorGroup box 1Immunogenic cell deathI IFN receptorATP-degrading enzymeSecretion of ATPMelanoma cell viabilityCognate immunityUVA irradiationAnticancer immunityImmunocompetent miceCalreticulin exposure
2018
Extracorporeal Photochemotherapy Drives Monocyte-to-Dendritic Cell Maturation to Induce Anti-Cancer Immunity
Ventura A, Vassall A, Robinson E, Filler R, Hanlon D, Meeth K, Ezaldein H, Girardi M, Sobolev O, Bosenberg MW, Edelson RL. Extracorporeal Photochemotherapy Drives Monocyte-to-Dendritic Cell Maturation to Induce Anti-Cancer Immunity. Cancer Research 2018, 78: canres.0171.2018. PMID: 29764863, DOI: 10.1158/0008-5472.can-18-0171.Peer-Reviewed Original ResearchConceptsT cellsT cell antitumor immunityTumor-specific T cellsTumor cellsEffective immunotherapeutic agentFavorable safety profileResponder T cellsDendritic cell differentiationTumor-challenged miceImmunogenic cell deathSelective antitumor effectApoptotic tumor cellsPotential therapeutic applicabilityProcessing/presentationAntimelanoma immunityHealthy DCsImmunogenic malignanciesAntitumor immunityCellular vaccinesImmunotherapeutic effectsAdditional malignanciesImmunotherapeutic agentsSafety profileCancer immunotherapyTumor antigens
2016
Quantifying in vivo murine antigen-specific T cell responses without requirement for prior knowledge of antigen identity
Kibbi N, Hong E, Ezaldein H, Hanlon D, Fahmy T, Edelson R. Quantifying in vivo murine antigen-specific T cell responses without requirement for prior knowledge of antigen identity. Transfusion And Apheresis Science 2016, 56: 179-189. PMID: 28007431, DOI: 10.1016/j.transci.2016.11.004.Peer-Reviewed Original ResearchConceptsCutaneous T-cell lymphomaExtracorporeal photochemotherapyCalcium fluxT cellsAntigen-specific T cell responsesMalignant cellsPatient-specific tumor antigensOVA-specific T cellsAntigen-specific T cellsAntigen-specific T cell activationControl recipient micePeptide-loaded DCImmune-based therapiesAnti-tumor responseT cell responsesAnti-cancer immunotherapyT-cell lymphomaT cell engagementT cell activationT cell receptor engagementPatient-specific responsesAdoptive transferClinical responseLymph nodesPeripheral blood
2015
Configuration-dependent Presentation of Multivalent IL-15:IL-15Rα Enhances the Antigen-specific T Cell Response and Anti-tumor Immunity*
Hong E, Usiskin IM, Bergamaschi C, Hanlon DJ, Edelson RL, Justesen S, Pavlakis GN, Flavell RA, Fahmy TM. Configuration-dependent Presentation of Multivalent IL-15:IL-15Rα Enhances the Antigen-specific T Cell Response and Anti-tumor Immunity*. Journal Of Biological Chemistry 2015, 291: 8931-8950. PMID: 26719339, PMCID: PMC4861462, DOI: 10.1074/jbc.m115.695304.Peer-Reviewed Original ResearchConceptsT cell responsesArtificial antigen-presenting cellsDendritic cellsIL-15Antigen-presenting cellsIL-15RαCell responsesAntigen-specific T cell responsesAntigen-processing dendritic cellsMaximal T cell responsesAnti-tumor immunitySame dendritic cellOptimal immune responseIL-15 functionsMechanism of actionIL-2Antigen deliveryImmune responseDC surfaceParacrine fashionTumor progressionMurine melanomaCellular mechanismsAggressive modelEnhanced potency
2012
Optimization of Stability, Encapsulation, Release, and Cross-Priming of Tumor Antigen-Containing PLGA Nanoparticles
Prasad S, Cody V, Saucier-Sawyer JK, Fadel TR, Edelson RL, Birchall MA, Hanlon DJ. Optimization of Stability, Encapsulation, Release, and Cross-Priming of Tumor Antigen-Containing PLGA Nanoparticles. Pharmaceutical Research 2012, 29: 2565-2577. PMID: 22798259, PMCID: PMC4075113, DOI: 10.1007/s11095-012-0787-4.Peer-Reviewed Original Research
2007
Polymer Nanoparticles for Immunotherapy from Encapsulated Tumor-Associated Antigens and Whole Tumor Cells
Solbrig CM, Saucier-Sawyer JK, Cody V, Saltzman WM, Hanlon DJ. Polymer Nanoparticles for Immunotherapy from Encapsulated Tumor-Associated Antigens and Whole Tumor Cells. Molecular Pharmaceutics 2007, 4: 47-57. PMID: 17217312, DOI: 10.1021/mp060107e.Peer-Reviewed Original ResearchConceptsPolymer nanoparticlesMixture of proteinsMolecular weight polymersRate of nanoparticlesProtein loadingEfficiency of encapsulationWeight polymersDissolved nanoparticlesNanoparticle formulationDifferent particle loadingsRelease propertiesNanoparticlesPreparation methodEncapsulation efficiencyComplex mixturesMolecular weightSDS-acrylamide gel electrophoresisSolvent extractionProtein assaysAgEncapsulationParticle loadingMixtureSpectrum of proteinsPromising approach
2005
Enhanced and prolonged cross‐presentation following endosomal escape of exogenous antigens encapsulated in biodegradable nanoparticles
Shen H, Ackerman AL, Cody V, Giodini A, Hinson ER, Cresswell P, Edelson RL, Saltzman WM, Hanlon DJ. Enhanced and prolonged cross‐presentation following endosomal escape of exogenous antigens encapsulated in biodegradable nanoparticles. Immunology 2005, 117: 78-88. PMID: 16423043, PMCID: PMC1782199, DOI: 10.1111/j.1365-2567.2005.02268.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigen PresentationB-LymphocytesBiocompatible MaterialsBiodegradation, EnvironmentalCell LineCross-PrimingDendritic CellsEndosomesHistocompatibility Antigens Class IIHumansLactic AcidLymphocyte ActivationMiceMice, Inbred C57BLNanostructuresOvalbuminPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerPolymersSerum Albumin, BovineT-LymphocytesConceptsBone marrow-derived dendritic cellsMHC class I presentationAntigen-presenting cellsClass I presentationMHC class IExogenous antigensDendritic cellsClass IAntigen deliveryPrimary mouse bone marrow-derived dendritic cellsSoluble antigenMouse bone marrow-derived dendritic cellsMarrow-derived dendritic cellsProfessional antigen-presenting cellsMajor histocompatibility complex class IProtein-based vaccinationT cell responsesClassic MHC class IExogenous antigen presentationHistocompatibility complex class IAntigen-coated latex beadsCell-associated antigensInterleukin-2 secretionComplex class IEfficiency of presentation