2019
Extracorporeal photochemotherapy induces bona fide immunogenic cell death
Tatsuno K, Yamazaki T, Hanlon D, Han P, Robinson E, Sobolev O, Yurter A, Rivera-Molina F, Arshad N, Edelson RL, Galluzzi L. Extracorporeal photochemotherapy induces bona fide immunogenic cell death. Cell Death & Disease 2019, 10: 578. PMID: 31371700, PMCID: PMC6675789, DOI: 10.1038/s41419-019-1819-3.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsAntigens, NeoplasmApoptosisCD8-Positive T-LymphocytesCell DifferentiationCell Line, TumorCell SurvivalDendritic CellsHMGB1 ProteinHumansImmunogenic Cell DeathLeukocytesLymphoma, T-Cell, CutaneousMethoxsalenMiceMonocytesPhotopheresisPhotosensitizing AgentsReceptor, Interferon alpha-betaUltraviolet RaysConceptsHigh mobility group box 1Tumor-associated antigensCutaneous T-cell lymphomaWhite blood cellsDendritic cellsImmunostimulatory signalsI interferonBona fide immunogenic cell deathMobility group box 1Such dendritic cellsSyngeneic immunocompetent miceCancer cellsT-cell lymphomaType I IFN receptorGroup box 1Immunogenic cell deathI IFN receptorATP-degrading enzymeSecretion of ATPMelanoma cell viabilityCognate immunityUVA irradiationAnticancer immunityImmunocompetent miceCalreticulin exposure
2015
Configuration-dependent Presentation of Multivalent IL-15:IL-15Rα Enhances the Antigen-specific T Cell Response and Anti-tumor Immunity*
Hong E, Usiskin IM, Bergamaschi C, Hanlon DJ, Edelson RL, Justesen S, Pavlakis GN, Flavell RA, Fahmy TM. Configuration-dependent Presentation of Multivalent IL-15:IL-15Rα Enhances the Antigen-specific T Cell Response and Anti-tumor Immunity*. Journal Of Biological Chemistry 2015, 291: 8931-8950. PMID: 26719339, PMCID: PMC4861462, DOI: 10.1074/jbc.m115.695304.Peer-Reviewed Original ResearchConceptsT cell responsesArtificial antigen-presenting cellsDendritic cellsIL-15Antigen-presenting cellsIL-15RαCell responsesAntigen-specific T cell responsesAntigen-processing dendritic cellsMaximal T cell responsesAnti-tumor immunitySame dendritic cellOptimal immune responseIL-15 functionsMechanism of actionIL-2Antigen deliveryImmune responseDC surfaceParacrine fashionTumor progressionMurine melanomaCellular mechanismsAggressive modelEnhanced potency
2011
Enhanced Stimulation of Anti‐Ovarian Cancer CD8+ T Cells by Dendritic Cells Loaded with Nanoparticle Encapsulated Tumor Antigen
Hanlon DJ, Aldo PB, Devine L, Alvero AB, Engberg AK, Edelson R, Mor G. Enhanced Stimulation of Anti‐Ovarian Cancer CD8+ T Cells by Dendritic Cells Loaded with Nanoparticle Encapsulated Tumor Antigen. American Journal Of Reproductive Immunology 2011, 65: 597-609. PMID: 21241402, PMCID: PMC3082607, DOI: 10.1111/j.1600-0897.2010.00968.x.Peer-Reviewed Original ResearchMeSH KeywordsAntigen PresentationAntigens, DifferentiationAntigens, NeoplasmCarcinomaCD8-Positive T-LymphocytesCells, CulturedCytokinesDendritic CellsFemaleHumansImmunotherapy, AdoptiveLactic AcidLymphocyte ActivationNanoparticlesOvarian NeoplasmsPolyglycolic AcidPolylactic Acid-Polyglycolic Acid CopolymerConceptsTumor-associated antigensT cell responsesT cellsDendritic cellsCytokine productionTumor antigensAnti-tumor T cell responsesCell surface co-stimulatory moleculesCD8 T cell responsesSurface co-stimulatory moleculesAnti-tumor immune responseAntigen-loaded DCsTumor lysate antigensCD8 T cellsCo-stimulatory moleculesT cell expressionHuman DCsActivation markersTumor lysateImmune responseLysate antigenBlood monocytesClinical testingEnhanced stimulationAntigen
2007
Polymer Nanoparticles for Immunotherapy from Encapsulated Tumor-Associated Antigens and Whole Tumor Cells
Solbrig CM, Saucier-Sawyer JK, Cody V, Saltzman WM, Hanlon DJ. Polymer Nanoparticles for Immunotherapy from Encapsulated Tumor-Associated Antigens and Whole Tumor Cells. Molecular Pharmaceutics 2007, 4: 47-57. PMID: 17217312, DOI: 10.1021/mp060107e.Peer-Reviewed Original ResearchConceptsPolymer nanoparticlesMixture of proteinsMolecular weight polymersRate of nanoparticlesProtein loadingEfficiency of encapsulationWeight polymersDissolved nanoparticlesNanoparticle formulationDifferent particle loadingsRelease propertiesNanoparticlesPreparation methodEncapsulation efficiencyComplex mixturesMolecular weightSDS-acrylamide gel electrophoresisSolvent extractionProtein assaysAgEncapsulationParticle loadingMixtureSpectrum of proteinsPromising approach