1982
Inelastic neutron scattering analysis of hexokinase dynamics and its modification on binding of glucose
Jacrot B, Cusack S, Dianoux A, Engelman D. Inelastic neutron scattering analysis of hexokinase dynamics and its modification on binding of glucose. Nature 1982, 300: 84-86. PMID: 6752726, DOI: 10.1038/300084a0.Peer-Reviewed Original ResearchMeSH KeywordsBinding SitesGlucoseHexokinaseNeutronsProtein BindingProtein ConformationSaccharomyces cerevisiaeScattering, RadiationConceptsInelastic neutron scatteringInelastic neutronField of biophysicsAtomic motionNeutron scatteringDynamical informationDynamical behaviorLimited experimental informationExperimental informationTemperature dependenceBiological macromoleculesWide frequencyTheoretical understandingMean positionNeutronsScatteringDynamicsInternal mobilityMotionDependenceBiophysicsFrequencyFluctuationsFieldExistence
1979
Substrate binding closes the cleft between the domains of yeast phosphoglycerate kinase.
Pickover C, McKay D, Engelman D, Steitz T. Substrate binding closes the cleft between the domains of yeast phosphoglycerate kinase. Journal Of Biological Chemistry 1979, 254: 11323-11329. PMID: 387770, DOI: 10.1016/s0021-9258(19)86488-8.Peer-Reviewed Original ResearchConceptsYeast phosphoglycerate kinasePhosphoglycerate kinaseConformational changesTernary complexSubstrate bindingHinge motionKinaseSubstrate MgATPCleft closureSmall-angle X-raySeparate bindingRadius of gyrationAngle X-rayMgATPBindingApparent similarityComplexesCleftEnzymeObserved changesHexokinaseGyration decreasesDomainSimilaritySmall angle X-ray scattering of dimeric yeast hexokinase in solution.
McDonald R, Engelman D, Steitz T. Small angle X-ray scattering of dimeric yeast hexokinase in solution. Journal Of Biological Chemistry 1979, 254: 2942-2943. PMID: 372185, DOI: 10.1016/s0021-9258(17)30165-5.Peer-Reviewed Original ResearchMeSH KeywordsHexokinaseMacromolecular SubstancesProtein ConformationSaccharomyces cerevisiaeX-Ray DiffractionYeast hexokinase in solution exhibits a large conformational change upon binding glucose or glucose 6-phosphate.
McDonald R, Steitz T, Engelman D. Yeast hexokinase in solution exhibits a large conformational change upon binding glucose or glucose 6-phosphate. Biochemistry 1979, 18: 338-42. PMID: 369601, DOI: 10.1021/bi00569a017.Peer-Reviewed Original ResearchGlucoseGlucosephosphatesHexokinaseProtein BindingProtein ConformationSaccharomyces cerevisiaeScattering, RadiationX-Rays