2006
Isolation and Identification of Membrane Protein Oligomers from the E. coli Inner Membrane
Stanley B, Engelman D. Isolation and Identification of Membrane Protein Oligomers from the E. coli Inner Membrane. The FASEB Journal 2006, 20: lb76-lb77. DOI: 10.1096/fasebj.20.5.lb76-d.Peer-Reviewed Original ResearchMembrane protein oligomersMembrane proteinsOligomeric complexesHelical membrane protein structuresNatural bilayersProtein oligomersE. coli inner membraneMembrane protein structuresMembrane protein complexesProtein complex associationsAssembly of proteinsF0 subunitsProtein complexesTransmembrane domainATP synthaseInner membraneFunctional complexProtein movementTransporter complexProtein structureFunctional studiesProtein orientationProteinComplex formationMembrane
1997
Assessment of the aggregation state of integral membrane proteins in reconstituted phospholipid vesicles using small angle neutron scattering11Edited by M. F. Moody
Hunt J, McCrea P, Zaccaı̈ G, Engelman D. Assessment of the aggregation state of integral membrane proteins in reconstituted phospholipid vesicles using small angle neutron scattering11Edited by M. F. Moody. Journal Of Molecular Biology 1997, 273: 1004-1019. PMID: 9367787, DOI: 10.1006/jmbi.1997.1330.Peer-Reviewed Original ResearchConceptsMembrane protein complexesIntegral membrane proteinsProtein complexesMembrane proteinsIntegral membrane protein complexPhospholipid vesiclesSmall unilamellar phospholipid vesiclesUnilamellar phospholipid vesiclesMolecular massF. MoodySpatial arrangementNon-ionic detergentIndividual complexesVesiclesModel systemMonomeric bacteriorhodopsinProteinUnknown scopeComplexesAggregation stateRadius of gyrationBacteriorhodopsinDetergentsBilayers
1995
Structural Model of the Phospholamban Ion Channel Complex in Phospholipid Membranes
Arkin I, Rothman M, Ludlam C, Aimoto S, Engelman D, Rothschild K, Smith S. Structural Model of the Phospholamban Ion Channel Complex in Phospholipid Membranes. Journal Of Molecular Biology 1995, 248: 824-834. PMID: 7752243, DOI: 10.1006/jmbi.1995.0263.Peer-Reviewed Original ResearchConceptsSelective ion conductanceTransmembrane domainAmino acid residuesN-terminal 30 amino acid residuesAcid residuesCircular dichroismPentameric protein complexFull-length proteinC-terminal 22 amino acid residuesPhospholipid membranesIon channel complexTransmembrane helicesProtein complexesPhosphorylation sitesMembrane proteinsIon conductanceCarboxy terminusHelix bundleIon poreReticulum membraneInhibitory complexLong helixPentameric complexSecondary structureProtein
1992
Forces involved in the assembly and stabilization of membrane proteins1
Cramer W, Engelman D, Von Heijne G, Rees D. Forces involved in the assembly and stabilization of membrane proteins1. The FASEB Journal 1992, 6: 3397-3402. PMID: 1464373, DOI: 10.1096/fasebj.6.15.1464373.Peer-Reviewed Original ResearchConceptsIntegral membrane proteinsHelix-helix interactionsMembrane proteinsIntegral membrane protein complexMembrane protein complexesMembrane-spanning domainsBacterial inner membraneSpecific dimer formationCytochrome b6f complexPolarity of residuesChloroplast thylakoid membranesWater-soluble proteinsProtein complexesReconstitution of bacteriorhodopsinHydrophobic subunitsInner membraneBacterial porinsB6f complexThylakoid membranesSuch polypeptidesBasic residuesProton translocationTrans sidePhotosynthetic reaction centersTrans-membrane orientation