1997
Spontaneous, pH-Dependent Membrane Insertion of a Transbilayer α-Helix †
Hunt J, Rath P, Rothschild K, Engelman D. Spontaneous, pH-Dependent Membrane Insertion of a Transbilayer α-Helix †. Biochemistry 1997, 36: 15177-15192. PMID: 9398245, DOI: 10.1021/bi970147b.Peer-Reviewed Original ResearchConceptsLipid bilayersIntegral membrane protein bacteriorhodopsinMembrane-spanning regionIntegral membrane proteinsPH-dependent membrane insertionAspartic acid residuesMembrane protein bacteriorhodopsinInsertion reactionMembrane insertionMembrane proteinsAqueous solutionHydrophobic sequenceAqueous bufferPoor solubilityAlpha-helixAcid residuesSignificant solubilityC-helixSpectroscopic assaysΑ-helixSecondary structureProtein bacteriorhodopsinNeutral pHPeptide associatesBilayers
1996
A Zinc-binding Domain Involved in the Dimerization of RAG1
Rodgers K, Bu Z, Fleming K, Schatz D, Engelman D, Coleman J. A Zinc-binding Domain Involved in the Dimerization of RAG1. Journal Of Molecular Biology 1996, 260: 70-84. PMID: 8676393, DOI: 10.1006/jmbi.1996.0382.Peer-Reviewed Original ResearchConceptsRecombination-activating gene 1Zinc-binding motifDimerization domainZinc fingerProtein-protein interactionsLymphoid-specific genesN-terminal thirdZinc finger sequencesAmino acid residuesC3HC4 motifRAG1 sequencesRAG1 proteinTerminal domainHomodimer formationAcid residuesBiophysical techniquesGene 1Energetics of associationMonomeric subunitsMotifProteinFinger sequencesSequenceC3HC4Zinc ions
1995
Structural Model of the Phospholamban Ion Channel Complex in Phospholipid Membranes
Arkin I, Rothman M, Ludlam C, Aimoto S, Engelman D, Rothschild K, Smith S. Structural Model of the Phospholamban Ion Channel Complex in Phospholipid Membranes. Journal Of Molecular Biology 1995, 248: 824-834. PMID: 7752243, DOI: 10.1006/jmbi.1995.0263.Peer-Reviewed Original ResearchConceptsSelective ion conductanceTransmembrane domainAmino acid residuesN-terminal 30 amino acid residuesAcid residuesCircular dichroismPentameric protein complexFull-length proteinC-terminal 22 amino acid residuesPhospholipid membranesIon channel complexTransmembrane helicesProtein complexesPhosphorylation sitesMembrane proteinsIon conductanceCarboxy terminusHelix bundleIon poreReticulum membraneInhibitory complexLong helixPentameric complexSecondary structureProtein