2024
Single-cell analysis reveals transcriptional dynamics in healthy primary parathyroid tissue.
Venkat A, Carlino M, Lawton B, Prasad M, Amodio M, Gibson C, Zeiss C, Youlten S, Krishnaswamy S, Krause D. Single-cell analysis reveals transcriptional dynamics in healthy primary parathyroid tissue. Genome Research 2024, 34: 837-850. PMID: 38977309, PMCID: PMC11293540, DOI: 10.1101/gr.278215.123.Peer-Reviewed Original ResearchCell statesMitochondrial transcript abundanceParathyroid glandsHuman parathyroidCell-cell communication analysisRNA expression analysisSingle-cell analysisTranscriptional dynamicsTranscript abundanceExpression dynamicsRNA transcriptomeEpithelial cell statesCell abundanceExpression analysisPseudotime analysis
2019
Low iron promotes megakaryocytic commitment of megakaryocytic-erythroid progenitors in humans and mice
Xavier-Ferrucio J, Scanlon V, Li X, Zhang PX, Lozovatsky L, Ayala-Lopez N, Tebaldi T, Halene S, Cao C, Fleming MD, Finberg KE, Krause DS. Low iron promotes megakaryocytic commitment of megakaryocytic-erythroid progenitors in humans and mice. Blood 2019, 134: 1547-1557. PMID: 31439541, PMCID: PMC6839952, DOI: 10.1182/blood.2019002039.Peer-Reviewed Original ResearchConceptsMK lineage commitmentExtracellular signal-regulated kinase (ERK) pathwaySignal-regulated kinase pathwayMegakaryocytic-erythroid progenitorsBone marrow transplantation assaysSignal transduction analysisIron-deficient conditionsGene expression analysisMegakaryocytic commitmentLineage commitmentTransferrin receptor 2MK lineageTmprss6-/- miceIron sensorExpression analysisKinase pathwayTransduction analysisTransplantation assaysErythroid progenitorsMarrow environmentHematopoietic cellsMessenger RNAPhospho-ERK1/2Systemic iron deficiencyLow iron
2016
Adult human megakaryocyte-erythroid progenitors are in the CD34+CD38mid fraction
Sanada C, Xavier-Ferrucio J, Lu YC, Min E, Zhang PX, Zou S, Kang E, Zhang M, Zerafati G, Gallagher PG, Krause DS. Adult human megakaryocyte-erythroid progenitors are in the CD34+CD38mid fraction. Blood 2016, 128: 923-933. PMID: 27268089, PMCID: PMC4990855, DOI: 10.1182/blood-2016-01-693705.Peer-Reviewed Original ResearchConceptsMegakaryocyte/erythroid progenitorsComparative expression analysisNovel enrichment strategyMegakaryocyte-erythroid progenitorsPurification strategySingle-cell levelShort hairpin RNAFate decisionsE lineageNovel purification strategyLineage fateLineage commitmentGranulocyte colony-stimulating factor-mobilized peripheral bloodMK lineageExpression analysisE progenitorsErythroid lineageFactor-mobilized peripheral bloodDifferential expressionES cellsErythroid progenitorsMYB knockdownHairpin RNALineagesColony-forming units
2002
Development of a murine hematopoietic progenitor complementary DNA microarray using a subtracted complementary DNA library
Ma X, Husain T, Peng H, Lin S, Mironenko O, Maun N, Johnson S, Tuck D, Berliner N, Krause DS, Perkins AS. Development of a murine hematopoietic progenitor complementary DNA microarray using a subtracted complementary DNA library. Blood 2002, 100: 833-844. PMID: 12130493, DOI: 10.1182/blood.v100.3.833.Peer-Reviewed Original ResearchConceptsMyeloid cell differentiationCell differentiationCDNA libraryGene expressionPrimary murine bone marrow cellsSignal transduction genesTypes of genesMurine bone marrow cellsComplementary DNA cloneGenomewide expression analysisStem cell differentiationComplementary DNA libraryComplementary DNA microarrayEML cellsTransduction genesHematopoietic genesUncharacterized ESTsSequence tagsDistinct genesDNA libraryDNA clonesTranscription factorsBone marrow-derived progenitorsExpression analysisDNA microarrays