2023
Electrical properties of dendritic spines
Zecevic D. Electrical properties of dendritic spines. Biophysical Journal 2023, 122: 4303-4315. PMID: 37837192, PMCID: PMC10698282, DOI: 10.1016/j.bpj.2023.10.008.Peer-Reviewed Original ResearchConceptsDendritic spinesIntracellular calcium concentration changesCortical pyramidal neuronsExcitatory synaptic transmissionCalcium concentration changesBasal dendritesPyramidal neuronsMushroom spinesSpine synapsesSynaptic transmissionBrain slicesVoltage-sensitive dyeNervous systemSpine neckSignificant physiological roleSynaptic signalingTwo-photon uncagingElectrical compartmentsSpineOnly experimental studiesBiochemical compartmentalizationPhysiological roleDifferent preparationsAnatomical structuresAdequate sensitivity
2022
Action Potential Initiation
Zecevic D, Popovic M. Action Potential Initiation. 2022, 156-161. DOI: 10.1007/978-1-0716-1006-0_237.Peer-Reviewed Original Research
2019
Using holographic illumination to study synaptic signal integration at individual dendritic spines
Weng J, Celis C, Zecevic D. Using holographic illumination to study synaptic signal integration at individual dendritic spines. 2019, 10865: 1086514. DOI: 10.1117/12.2507284.Peer-Reviewed Original ResearchSynaptic signal integrationExcitatory synaptic potentialsDendritic spinesSynaptic potentialsTemporal summationAcute cortical brain slicesIndividual synapsesLayer 5 pyramidal neuronsIndividual excitatory synapsesThin basal dendritesCortical brain slicesVoltage-sensitive dye recordingIndividual dendritic spinesSite of originSensory information processingBasal dendritesPyramidal neuronsExcitatory synapsesBrain slicesRepetitive activationVoltage-sensitive dyeSomatic recordingsSingle synapsesSynaptic signalsIndividual neurons
2017
The stochastic nature of action potential backpropagation in apical tuft dendrites
Short SM, Oikonomou KD, Zhou WL, Acker CD, Popovic MA, Zecevic D, Antic SD. The stochastic nature of action potential backpropagation in apical tuft dendrites. Journal Of Neurophysiology 2017, 118: 1394-1414. PMID: 28566465, PMCID: PMC5558024, DOI: 10.1152/jn.00800.2016.Peer-Reviewed Original ResearchConceptsDendritic CaPyramidal neuronsApical tuftCortical pyramidal neuronsAction potential backpropagationRat brain slicesSpontaneous synaptic inputsDifferent cortical layersVoltage-gated CaAP backpropagationAP burstsGlutamate iontophoresisSynaptic contactsSomatic APsApical trunkAP frequencyBrain slicesDendritic physiologySynaptic inputsCortical layersChannel inactivationLocal NaNeuronsSpike-timing dependent plasticityTrialsImaging membrane potential changes from dendritic spines using computer-generated holography
Tanese D, Weng JY, Zampini V, De Sars V, Canepari M, Rozsa B, Emiliani V, Zecevic D. Imaging membrane potential changes from dendritic spines using computer-generated holography. Neurophotonics 2017, 4: 031211-031211. PMID: 28523281, PMCID: PMC5428833, DOI: 10.1117/1.nph.4.3.031211.Peer-Reviewed Original Research
2016
FHF-independent conduction of action potentials along the leak-resistant cerebellar granule cell axon
Dover K, Marra C, Solinas S, Popovic M, Subramaniyam S, Zecevic D, D’Angelo E, Goldfarb M. FHF-independent conduction of action potentials along the leak-resistant cerebellar granule cell axon. Nature Communications 2016, 7: 12895. PMID: 27666389, PMCID: PMC5052690, DOI: 10.1038/ncomms12895.Peer-Reviewed Original ResearchCentral nervous systemAction potentialsNervous systemSodium action potentialsGranule cell axonsAxon initial segmentCerebellar granule cellsNodes of RanvierCell axonsSomatodendritic membraneVertebrate central nervous systemGranule cellsCerebellar granule cell axonsDistal axonsSpike conductionAxonsSodium channels
2015
Electrical behaviour of dendritic spines as revealed by voltage imaging
Popovic MA, Carnevale N, Rozsa B, Zecevic D. Electrical behaviour of dendritic spines as revealed by voltage imaging. Nature Communications 2015, 6: 8436. PMID: 26436431, PMCID: PMC4594633, DOI: 10.1038/ncomms9436.Peer-Reviewed Original ResearchAction Potential Initiation
Zecevic D, Popovic M. Action Potential Initiation. 2015, 137-142. DOI: 10.1007/978-1-4614-6675-8_237.Peer-Reviewed Original ResearchPioneers in Neurophotonics: Special Section Honoring Professor Lawrence B. Cohen
Salzberg BM, Zecevic D. Pioneers in Neurophotonics: Special Section Honoring Professor Lawrence B. Cohen. Neurophotonics 2015, 2: 021001-021001. PMID: 26157992, PMCID: PMC4487646, DOI: 10.1117/1.nph.2.2.021001.Peer-Reviewed Original ResearchImaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines
Popovic M, Vogt K, Holthoff K, Konnerth A, Salzberg BM, Grinvald A, Antic SD, Canepari M, Zecevic D. Imaging Submillisecond Membrane Potential Changes from Individual Regions of Single Axons, Dendrites and Spines. Advances In Experimental Medicine And Biology 2015, 859: 57-101. PMID: 26238049, PMCID: PMC5671121, DOI: 10.1007/978-3-319-17641-3_3.Peer-Reviewed Original ResearchConceptsIndividual neuronsMembrane potential changesVoltage-sensitive dye recordingAction potential initiationIndividual dendritic spinesSite of originAxon collateralsIndividual nerve cellsMembrane potential transientsVoltage-sensitive dyeDendritic spinesRegional electrical propertiesDendritic treeNerve cellsNeuronal processesSingle axonsPotential initiationComplex operational unitsBehavioral modificationNeuronal network analysisNeuronsInput-output functionMultisite recordingsPreface.
Canepari M, Bernus O, Zecevic D. Preface. 2015, 859: v-vi. PMID: 26512376.Peer-Reviewed Original ResearchCombining Membrane Potential Imaging with Other Optical Techniques
Jaafari N, Vogt KE, Saggau P, Leslie LM, Zecevic D, Canepari M. Combining Membrane Potential Imaging with Other Optical Techniques. Advances In Experimental Medicine And Biology 2015, 859: 103-125. PMID: 26238050, PMCID: PMC5675139, DOI: 10.1007/978-3-319-17641-3_4.Peer-Reviewed Original ResearchConceptsMembrane potential imagingOptical techniquesPotential imagingUncaging techniquesChannelrhodopsin stimulationVoltage-sensitive dyeVariety of applicationsSpatial mappingElectrical signalsImportant applicationsVoltageFluorescence intensityImagingParticular moleculeSignalsMembrane potential changesGateNovel investigationOptogeneticsMeasurementsIntensityTechniqueCurrent
2014
Imaging with organic indicators and high-speed charge-coupled device cameras in neurons: some applications where these classic techniques have advantages
Ross WN, Miyazaki K, Popovic MA, Zecevic D. Imaging with organic indicators and high-speed charge-coupled device cameras in neurons: some applications where these classic techniques have advantages. Neurophotonics 2014, 2: 021005-021005. PMID: 26157996, PMCID: PMC4478887, DOI: 10.1117/1.nph.2.2.021005.Peer-Reviewed Original ResearchAction Potential Initiation
Zecevic D, Popovic M. Action Potential Initiation. 2014, 1-6. DOI: 10.1007/978-1-4614-7320-6_237-1.Peer-Reviewed Original Research
2013
Combining calcium imaging with other optical techniques.
Canepari M, Zecevic D, Vogt KE, Ogden D, De Waard M. Combining calcium imaging with other optical techniques. Cold Spring Harbor Protocols 2013, 2013: 1125-31. PMID: 24298025, PMCID: PMC5225261, DOI: 10.1101/pdb.top066167.Peer-Reviewed Original ResearchCombining Ca2+ and membrane potential imaging in single neurons.
Canepari M, Vogt KE, De Waard M, Zecevic D. Combining Ca2+ and membrane potential imaging in single neurons. Cold Spring Harbor Protocols 2013, 2013: 1161-4. PMID: 24298027, PMCID: PMC5225264, DOI: 10.1101/pdb.prot073114.Peer-Reviewed Original Research
2012
Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices.
Popovic M, Gao X, Zecevic D. Voltage-sensitive dye recording from axons, dendrites and dendritic spines of individual neurons in brain slices. Journal Of Visualized Experiments 2012, e4261. PMID: 23222505, PMCID: PMC3565854, DOI: 10.3791/4261.Peer-Reviewed Original ResearchConceptsLaser light sourceSub-micrometer resolutionLight sourceOptical recordingDetectable pharmacological effectsCurrent sensitivityVoltage-sensitive dyeSubthreshold eventsElectrical propertiesDetailed measurementsMultiple-site optical recordingVoltage-sensitive dye recordingFluorescence signalOptimal wavelengthsMembrane potential transientsElectrical signalsIndividual dendritic spinesExperimental evidenceWavelengthMeasurement oneMeasurementsBroadeningDye loadingPotential transientsBrightnessCortical Dendritic Spine Heads Are Not Electrically Isolated by the Spine Neck from Membrane Potential Signals in Parent Dendrites
Popovic MA, Gao X, Carnevale NT, Zecevic D. Cortical Dendritic Spine Heads Are Not Electrically Isolated by the Spine Neck from Membrane Potential Signals in Parent Dendrites. Cerebral Cortex 2012, 24: 385-395. PMID: 23054810, PMCID: PMC3888368, DOI: 10.1093/cercor/bhs320.Peer-Reviewed Original ResearchHigh Signal-to-Noise Ratio Voltage Imaging: A Powerful Tool for Determining Electrophysiological Properties of CNS Axons
Foust A, Casale A, Zecevic D, McCormick D. High Signal-to-Noise Ratio Voltage Imaging: A Powerful Tool for Determining Electrophysiological Properties of CNS Axons. 2012, btu4a.3. DOI: 10.1364/biomed.2012.btu4a.3.Peer-Reviewed Original Research
2011
Somatic Membrane Potential and Kv1 Channels Control Spike Repolarization in Cortical Axon Collaterals and Presynaptic Boutons
Foust AJ, Yu Y, Popovic M, Zecevic D, McCormick DA. Somatic Membrane Potential and Kv1 Channels Control Spike Repolarization in Cortical Axon Collaterals and Presynaptic Boutons. Journal Of Neuroscience 2011, 31: 15490-15498. PMID: 22031895, PMCID: PMC3225031, DOI: 10.1523/jneurosci.2752-11.2011.Peer-Reviewed Original ResearchMeSH Keywords4-AminopyridineAnimalsAxonsBiophysical PhenomenaComputer SimulationCrystallinsDose-Response Relationship, DrugElapid VenomsElectric StimulationFemaleGreen Fluorescent ProteinsIn Vitro TechniquesMaleMembrane PotentialsMiceMice, TransgenicModels, NeurologicalMu-CrystallinsNerve NetNeuronsPatch-Clamp TechniquesPotassium Channel BlockersPresynaptic TerminalsShaker Superfamily of Potassium ChannelsSomatosensory CortexStyrenesTetraethylammoniumConceptsAxon collateralsPresynaptic boutonsKv1 channelsSpike repolarizationPresynaptic terminalsSubthreshold depolarizationAction potentialsΑ-dendrotoxinLayer 5 pyramidal cellsIntracortical axon collateralsSynaptic neurotransmitter releaseMouse brain slicesSomatic membrane potentialKv1 subunitsPyramidal cellsSynaptic transmissionBrain slicesVoltage-sensitive dyeLow dosesNeurotransmitter releaseSynaptic strengthBoutonsCollateralsRepolarizationIon channels