2024
Imaging chronic active lesions in multiple sclerosis: a consensus statement
Bagnato F, Sati P, Hemond C, Elliott C, Gauthier S, Harrison D, Mainero C, Oh J, Pitt D, Shinohara R, Smith S, Trapp B, Azevedo C, Calabresi P, Henry R, Laule C, Ontaneda D, Rooney W, Sicotte N, Reich D, Absinta M. Imaging chronic active lesions in multiple sclerosis: a consensus statement. Brain 2024, 147: 2913-2933. PMID: 38226694, PMCID: PMC11370808, DOI: 10.1093/brain/awae013.Peer-Reviewed Original ResearchChronic active lesionsMagnetic resonance imagingConsensus statementMultiple sclerosisActive lesionsManifestations of chronic inflammationParamagnetic rim lesionsMRI-definedNorth American ImagingRadiological definitionChronic inflammationRim lesionsClinical translationLesionsResonance imagingMRI sequencesIn vivoT1-wBrains of personsBiomarkers
2021
QSM is an imaging biomarker for chronic glial activation in multiple sclerosis lesions
Gillen KM, Mubarak M, Park C, Ponath G, Zhang S, Dimov A, Levine‐Ritterman M, Toro S, Huang W, Amici S, Kaunzner UW, Gauthier SA, Guerau‐de‐Arellano M, Wang Y, Nguyen TD, Pitt D. QSM is an imaging biomarker for chronic glial activation in multiple sclerosis lesions. Annals Of Clinical And Translational Neurology 2021, 8: 877-886. PMID: 33704933, PMCID: PMC8045922, DOI: 10.1002/acn3.51338.Peer-Reviewed Original ResearchConceptsNormal-appearing white matterMyeloid cellsLesion rimReactive oxygen speciesQuantitative susceptibility mappingChronic active lesionsChronic glial activationPro-inflammatory cytokinesBlood-brain barrierWhite matter lesionsAdjacent normal-appearing white matterMultiple sclerosis lesionsGlial activationActivated microgliaHistopathological correlatesChronic inflammationActive lesionsCytokine productionMatter lesionsMS lesionsWhite matterHuman-induced pluripotent stem cellsSclerosis lesionsLesionsLesion perimeter
2019
Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry
Ramaglia V, Sheikh-Mohamed S, Legg K, Park C, Rojas OL, Zandee S, Fu F, Ornatsky O, Swanson EC, Pitt D, Prat A, McKee TD, Gommerman JL. Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry. ELife 2019, 8: e48051. PMID: 31368890, PMCID: PMC6707785, DOI: 10.7554/elife.48051.Peer-Reviewed Original ResearchConceptsMultiple sclerosisMS disease activityT-cell phenotypeMass cytometryTypes of lymphocytesMultiple sclerosis lesionsNatalizumab cessationDisease activityMS patientsInflammatory lesionsImmune cellsSpinal cordLesion morphometryMS lesionsB cellsLesion typeSclerosis lesionsLesionsBlood vesselsCell phenotypeFunctional stateCytometryCellular contentCell-cell interactionsPhenotype
2018
The Role of Astrocytes in Multiple Sclerosis
Ponath G, Park C, Pitt D. The Role of Astrocytes in Multiple Sclerosis. Frontiers In Immunology 2018, 9: 217. PMID: 29515568, PMCID: PMC5826071, DOI: 10.3389/fimmu.2018.00217.Peer-Reviewed Original ResearchConceptsRole of astrocytesImmune cell accessCentral nervous systemMultiple sclerosis lesionsAstrocyte activationMultiple sclerosisGlial scarAstrocyte functionMS treatmentMS lesionsNervous systemAstrocytesSclerosis lesionsLesion formationFunctional polarizationLesionsCell accessSclerosisInflammationPathogenesis
2016
Quantitative Susceptibility Mapping and R2* Measured Changes during White Matter Lesion Development in Multiple Sclerosis: Myelin Breakdown, Myelin Debris Degradation and Removal, and Iron Accumulation
Zhang Y, Gauthier SA, Gupta A, Chen W, Comunale J, Chiang G, Zhou D, Askin G, Zhu W, Pitt D, Wang Y. Quantitative Susceptibility Mapping and R2* Measured Changes during White Matter Lesion Development in Multiple Sclerosis: Myelin Breakdown, Myelin Debris Degradation and Removal, and Iron Accumulation. American Journal Of Neuroradiology 2016, 37: 1629-1635. PMID: 27256856, PMCID: PMC5018433, DOI: 10.3174/ajnr.a4825.Peer-Reviewed Original ResearchConceptsMyelin breakdownQuantitative susceptibility mappingExpanded Disability Status Scale scoreEarly active MS lesionsDisability Status Scale scoreIron accumulationDifferent enhancing patternStatus Scale scoreActive MS lesionsWhite matter lesionsMultiple sclerosis lesionsDisease durationChronic lesionsEarly chronicMatter lesionsMultiple sclerosisActive lesionsMyelin debrisMS lesionsLesion changesScale scoreLesion typeSclerosis lesionsLesionsLesion development
2014
Quantitative susceptibility mapping (QSM) of white matter multiple sclerosis lesions: Interpreting positive susceptibility and the presence of iron
Wisnieff C, Ramanan S, Olesik J, Gauthier S, Wang Y, Pitt D. Quantitative susceptibility mapping (QSM) of white matter multiple sclerosis lesions: Interpreting positive susceptibility and the presence of iron. Magnetic Resonance In Medicine 2014, 74: 564-570. PMID: 25137340, PMCID: PMC4333139, DOI: 10.1002/mrm.25420.Peer-Reviewed Original ResearchConceptsMultiple sclerosisQuantitative susceptibility mappingIron depositionMS brain tissueMicroglia/macrophagesWhite matter multiple sclerosis lesionsPresence of myelinMultiple sclerosis lesionsSubstantial iron depositionChronic inflammationMS lesionsSclerosis lesionsLesionsBrain tissueMyelinMicroglia
2013
Quantitative Susceptibility Mapping of Multiple Sclerosis Lesions at Various Ages
Chen W, Gauthier SA, Gupta A, Comunale J, Liu T, Wang S, Pei M, Pitt D, Wang Y. Quantitative Susceptibility Mapping of Multiple Sclerosis Lesions at Various Ages. Radiology 2013, 271: 183-92. PMID: 24475808, PMCID: PMC4263629, DOI: 10.1148/radiol.13130353.Peer-Reviewed Original ResearchConceptsNormal-appearing white matterMS lesionsMultiple sclerosis lesionsEnhanced lesionsQuantitative susceptibility mappingMR examinationsSclerosis lesionsConventional magnetic resonance imagingMagnetic resonance imagingT2-weighted imagesMS patientsPathophysiologic featuresCerebrospinal fluidOnline supplemental materialWhite matterLesionsQSM imagesResonance imagingPatientsMR imagingThree-dimensional gradient-echo sequenceGradient echo sequenceMonthsBonferroni correctionAge
2012
Detecting cortical lesions in multiple sclerosis at 7 T using white matter signal attenuation
Bluestein KT, Pitt D, Sammet S, Zachariah CR, Nagaraj U, Knopp MV, Schmalbrock P. Detecting cortical lesions in multiple sclerosis at 7 T using white matter signal attenuation. Magnetic Resonance Imaging 2012, 30: 907-915. PMID: 22578928, PMCID: PMC3402634, DOI: 10.1016/j.mri.2012.03.006.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overAlgorithmsCerebral CortexFemaleHumansImage EnhancementImage Interpretation, Computer-AssistedMagnetic Resonance ImagingMaleMiddle AgedMultiple SclerosisNerve Fibers, MyelinatedReproducibility of ResultsSensitivity and SpecificitySubtraction TechniqueYoung Adult
2011
T1 and proton density at 7 T in patients with multiple sclerosis: an initial study
Bluestein KT, Pitt D, Knopp MV, Schmalbrock P. T1 and proton density at 7 T in patients with multiple sclerosis: an initial study. Magnetic Resonance Imaging 2011, 30: 19-25. PMID: 21937183, PMCID: PMC3375320, DOI: 10.1016/j.mri.2011.07.018.Peer-Reviewed Original ResearchConceptsCortical lesionsMultiple sclerosisWhite matterGray matterHealthy control subjectsWhite matter lesionsNormal-appearing tissueMS patientsMagnetic resonance sequencesMatter lesionsControl subjectsCerebrospinal fluidMS researchLesionsPatientsMR imagingUltrahigh-field MR imagingField MR imagingSclerosisProton densityResonance sequencesTissue responseTurbo fieldMagnetic resonanceInitial study
2010
Imaging Cortical Lesions in Multiple Sclerosis With Ultra–High-Field Magnetic Resonance Imaging
Pitt D, Boster A, Pei W, Wohleb E, Jasne A, Zachariah CR, Rammohan K, Knopp MV, Schmalbrock P. Imaging Cortical Lesions in Multiple Sclerosis With Ultra–High-Field Magnetic Resonance Imaging. JAMA Neurology 2010, 67: 812-818. PMID: 20625086, DOI: 10.1001/archneurol.2010.148.Peer-Reviewed Original ResearchConceptsCortical lesion detectionCortical lesionsMultiple sclerosisLesion typeCortical multiple sclerosis lesionsBrain tissueCortical lesion typesMultiple sclerosis tissueAutopsied brain tissueInversion recoveryMagnetic resonance imagingMultiple sclerosis lesionsCorresponding histological sectionsLesion detectionMyelin basic proteinHypointense ringUndetected lesionsImmunohistochemical analysisSclerosis lesionsLesionsResonance imagingSensitivity of T2SclerosisMagnetic resonance imagesLesion visibility
2001
Multiple sclerosis: Altered glutamate homeostasis in lesions correlates with oligodendrocyte and axonal damage
Werner P, Pitt D, Raine C. Multiple sclerosis: Altered glutamate homeostasis in lesions correlates with oligodendrocyte and axonal damage. Annals Of Neurology 2001, 50: 169-180. PMID: 11506399, DOI: 10.1002/ana.1077.Peer-Reviewed Original ResearchConceptsMultiple sclerosisAxonal damageWhite matterGlutamate excitotoxicityGlutamate homeostasisMS lesionsGlutaminase expressionMS white matterInflammatory neurologic diseasesActive MS lesionsCNS cell typesOligodendroglial pathologyNoninflammatory conditionsDystrophic axonsNeurologic diseaseGLT-1Low-level expressionAnimal modelsGlutamate transportersHomeostasis contributesLesion correlatesGlutamate transportLesionsOligodendrocytesTherapeutic import
1999
PERFORIN EXPRESSION IN DEMYELINATIVE LESIONS OF MULTIPLE SCLEROSIS, AND A ROLE FOR PERFORIN IN THE REGULATION OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS
Zeine R, Cannella B, Pitt D, Raine C. PERFORIN EXPRESSION IN DEMYELINATIVE LESIONS OF MULTIPLE SCLEROSIS, AND A ROLE FOR PERFORIN IN THE REGULATION OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS. Journal Of Neuropathology & Experimental Neurology 1999, 58: 564. DOI: 10.1097/00005072-199905000-00232.Peer-Reviewed Original Research