1997
Glomerular epithelial cell products stimulate mesangial cell proliferation in culture
Budde K, Neumayer H, Salant D, Cybulsky A, Coleman D, Sterzel R. Glomerular epithelial cell products stimulate mesangial cell proliferation in culture. Kidney International 1997, 52: 733-741. PMID: 9291194, DOI: 10.1038/ki.1997.389.Peer-Reviewed Original ResearchConceptsGlomerular epithelial cellsMC growth factorsMesangial cellsGlomerular diseaseGrowth factorMesangial cell proliferationEpithelial cell productsQuiescent rat mesangial cellsRat mesangial cellsIL-6IL-4IL-5TNF-alphaIL-2Glomerular cell typesIL-1GM-CSFTGF betaSoluble factorsProliferative activityCell productsCompetitive binding assaysEpithelial cellsMitogenic activityCell proliferation
1991
Transfection of adherent murine peritoneal macrophages with a reporter gene using DEAE-dextran
Rupprecht A, Coleman D. Transfection of adherent murine peritoneal macrophages with a reporter gene using DEAE-dextran. Journal Of Immunological Methods 1991, 144: 157-163. PMID: 1960412, DOI: 10.1016/0022-1759(91)90082-q.Peer-Reviewed Original ResearchConceptsReporter geneMurine peritoneal macrophagesBacterial chloramphenicol acetyl transferase (CAT) reporter geneChloramphenicol acetyl transferase (CAT) reporter geneAcetyl transferase reporter geneCAT reporter geneHeterologous plasmid DNAEukaryotic expression vectorReporter gene assayThioglycollate-elicited murine peritoneal macrophagesCAT activityHigher CAT activityTransfection of macrophagesGene regulationPeritoneal macrophagesPrimary culturesExpression vectorPrimary macrophage culturesGene assayTransfection strategyGenesTransfectionDEAE-dextranAdherent macrophagesPlasmid DNA
1990
Macrophage colony-stimulating factor production by murine and human keratinocytes. Enhancement by bacterial lipopolysaccharide.
Chodakewitz J, Lacy J, Edwards S, Birchall N, Coleman D. Macrophage colony-stimulating factor production by murine and human keratinocytes. Enhancement by bacterial lipopolysaccharide. The Journal Of Immunology 1990, 144: 2190-6. PMID: 2179407, DOI: 10.4049/jimmunol.144.6.2190.Peer-Reviewed Original ResearchRat Tracheal Epithelial Cells Produce Granulocyte/Macrophage Colony-stimulating Factor
Smith S, Lee D, Lacy J, Coleman D. Rat Tracheal Epithelial Cells Produce Granulocyte/Macrophage Colony-stimulating Factor. American Journal Of Respiratory Cell And Molecular Biology 1990, 2: 59-68. PMID: 2407273, DOI: 10.1165/ajrcmb/2.1.59.Peer-Reviewed Original ResearchAnimalsAntibodiesCells, CulturedChromatography, High Pressure LiquidColony-Stimulating FactorsCulture MediaDNA ProbesEpithelial CellsEpitheliumGranulocyte-Macrophage Colony-Stimulating FactorGrowth SubstancesLipopolysaccharidesMaleMolecular WeightNucleic Acid HybridizationRatsRats, Inbred F344TracheaTranscription, Genetic
1989
Rat mesangial cells produce granulocyte-macrophage colony-stimulating factor
Budde K, Coleman D, Lacy J, Sterzel R. Rat mesangial cells produce granulocyte-macrophage colony-stimulating factor. American Journal Of Physiology 1989, 257: f1065-f1078. PMID: 2690641, DOI: 10.1152/ajprenal.1989.257.6.f1065.Peer-Reviewed Original ResearchConceptsRat mesangial cellsGranulocyte-macrophage colony-stimulating factorCultured rat mesangial cellsMesangial cellsColony-stimulating factorGM-CSFMC-CMGlomerular mesangial cellsPeritoneal MPsEffector cellsIL-2Inflammatory processGlomerular diseaseInterleukin-2T cellsT lymphocytesInterleukin-4Murine GM-CSFRenal glomeruliSerum-free conditionsSoluble factorsProliferative activitySpecific cDNA probesGM-CSF mRNA transcriptsPresent findings
1987
Stimulation of macrophage H2O2 release by resident thymocytes: Effect of a soluble factor distinct from interferon-γ
Mellors J, Bartiss A, Coleman D. Stimulation of macrophage H2O2 release by resident thymocytes: Effect of a soluble factor distinct from interferon-γ. Cellular Immunology 1987, 110: 391-399. PMID: 3121188, DOI: 10.1016/0008-8749(87)90131-6.Peer-Reviewed Original ResearchConceptsT cellsResident thymocytesAntigen-specific immune responsesSoluble factorsMacrophage microbicidal mechanismsRespiratory burst capacityLow molecular weight soluble factorMacrophage oxidative metabolismNonactivated T cellsMacrophage H2O2 releaseActivated T cellsMacrophage effector functionsEnzyme-linked immunosorbentReceptor-mediated phagocytosisImmune responseIFN-gammaEffector functionsToxoplasma gondiiControl macrophagesRespiratory burstMicrobicidal mechanismsPeritoneal macrophagesInterferon activityMacrophagesBurst capacity