Identification of a Cellular Protein That Specifically Interacts with the Essential Cysteine Region of the HIV-1 Tat Transactivator
KAMINE J, ELANGOVAN B, SUBRAMANIAN T, COLEMAN D, CHINNADURAI G. Identification of a Cellular Protein That Specifically Interacts with the Essential Cysteine Region of the HIV-1 Tat Transactivator. Virology 1996, 216: 357-366. PMID: 8607265, DOI: 10.1006/viro.1996.0071.Peer-Reviewed Original ResearchMeSH KeywordsAcetyltransferasesAmino Acid SequenceBase SequenceCloning, MolecularConserved SequenceCysteineDNA, ComplementaryEscherichia coliGene Expression Regulation, ViralGene Products, tatHeLa CellsHistone AcetyltransferasesHIV-1HumansLysine Acetyltransferase 5Molecular Sequence DataPoint MutationPromoter Regions, GeneticProtein BindingProteinsSaccharomyces cerevisiaeTat Gene Products, Human Immunodeficiency VirusConceptsHIV gene expressionGene expressionN-terminal 31 amino acidsEssential cysteine residueFull-length cDNATat activation domainHIV-1 Tat transactivatorPartial cDNA cloneHIV-1 promoterActivation domainCellular proteinsTAT activityAbolishes interactionCysteine regionCDNA clonesCellular cofactorsCysteine residuesTransient expressionTat transactivationTat transactivatorHIV-1 TatAmino acidsInteractive cloningProteinPromoter