Transfection of adherent murine peritoneal macrophages with a reporter gene using DEAE-dextran
Rupprecht A, Coleman D. Transfection of adherent murine peritoneal macrophages with a reporter gene using DEAE-dextran. Journal Of Immunological Methods 1991, 144: 157-163. PMID: 1960412, DOI: 10.1016/0022-1759(91)90082-q.Peer-Reviewed Original ResearchConceptsReporter geneMurine peritoneal macrophagesBacterial chloramphenicol acetyl transferase (CAT) reporter geneChloramphenicol acetyl transferase (CAT) reporter geneAcetyl transferase reporter geneCAT reporter geneHeterologous plasmid DNAEukaryotic expression vectorReporter gene assayThioglycollate-elicited murine peritoneal macrophagesCAT activityHigher CAT activityTransfection of macrophagesGene regulationPeritoneal macrophagesPrimary culturesExpression vectorPrimary macrophage culturesGene assayTransfection strategyGenesTransfectionDEAE-dextranAdherent macrophagesPlasmid DNA