1992
Lipopolysaccharide induces Egr-1 mRNA and protein in murine peritoneal macrophages.
Coleman D, Bartiss A, Sukhatme V, Liu J, Rupprecht H. Lipopolysaccharide induces Egr-1 mRNA and protein in murine peritoneal macrophages. The Journal Of Immunology 1992, 149: 3045-51. PMID: 1401930, DOI: 10.4049/jimmunol.149.9.3045.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCell DivisionDNA-Binding ProteinsDose-Response Relationship, ImmunologicEarly Growth Response Protein 1Hydrogen PeroxideImmediate-Early ProteinsImmunity, CellularLipopolysaccharidesMacrophagesMiceMice, Inbred BALB CMolecular Sequence DataPeritoneal CavityProtein Kinase CRNA, MessengerTetradecanoylphorbol AcetateTime FactorsTranscription FactorsTranscription, GeneticTranscriptional ActivationZinc FingersConceptsProtein kinase CEgr-1 mRNAEgr-1 proteinEgr-1Early response gene familyMurine peritoneal macrophagesTranscription of genesThioglycollate-elicited murine peritoneal macrophages
1991
Transfection of adherent murine peritoneal macrophages with a reporter gene using DEAE-dextran
Rupprecht A, Coleman D. Transfection of adherent murine peritoneal macrophages with a reporter gene using DEAE-dextran. Journal Of Immunological Methods 1991, 144: 157-163. PMID: 1960412, DOI: 10.1016/0022-1759(91)90082-q.Peer-Reviewed Original ResearchConceptsReporter geneMurine peritoneal macrophagesBacterial chloramphenicol acetyl transferase (CAT) reporter geneChloramphenicol acetyl transferase (CAT) reporter geneAcetyl transferase reporter geneCAT reporter geneHeterologous plasmid DNAEukaryotic expression vectorReporter gene assayThioglycollate-elicited murine peritoneal macrophagesCAT activityHigher CAT activityTransfection of macrophagesGene regulationPeritoneal macrophagesPrimary culturesExpression vectorPrimary macrophage culturesGene assayTransfection strategyGenesTransfectionDEAE-dextranAdherent macrophagesPlasmid DNA