1996
Identification of a Cellular Protein That Specifically Interacts with the Essential Cysteine Region of the HIV-1 Tat Transactivator
KAMINE J, ELANGOVAN B, SUBRAMANIAN T, COLEMAN D, CHINNADURAI G. Identification of a Cellular Protein That Specifically Interacts with the Essential Cysteine Region of the HIV-1 Tat Transactivator. Virology 1996, 216: 357-366. PMID: 8607265, DOI: 10.1006/viro.1996.0071.Peer-Reviewed Original ResearchMeSH KeywordsAcetyltransferasesAmino Acid SequenceBase SequenceCloning, MolecularConserved SequenceCysteineDNA, ComplementaryEscherichia coliGene Expression Regulation, ViralGene Products, tatHeLa CellsHistone AcetyltransferasesHIV-1HumansLysine Acetyltransferase 5Molecular Sequence DataPoint MutationPromoter Regions, GeneticProtein BindingProteinsSaccharomyces cerevisiaeTat Gene Products, Human Immunodeficiency VirusConceptsHIV gene expressionGene expressionN-terminal 31 amino acidsEssential cysteine residueFull-length cDNATat activation domainHIV-1 Tat transactivatorPartial cDNA cloneHIV-1 promoterActivation domainCellular proteinsTAT activityAbolishes interactionCysteine regionCDNA clonesCellular cofactorsCysteine residuesTransient expressionTat transactivationTat transactivatorHIV-1 TatAmino acidsInteractive cloningProteinPromoter
1994
Serum response elements mediate protein kinase C dependent transcriptional induction of early growth response gene‐1 by arginine vasopressin in rat mesangial cells
Rupprecht H, Sukhatme V, Rupprecht A, Sterzel R, Coleman D. Serum response elements mediate protein kinase C dependent transcriptional induction of early growth response gene‐1 by arginine vasopressin in rat mesangial cells. Journal Of Cellular Physiology 1994, 159: 311-323. PMID: 8163570, DOI: 10.1002/jcp.1041590214.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArginine VasopressinBase SequenceBloodDNA-Binding ProteinsEarly Growth Response Protein 1Enzyme ActivationGene Expression RegulationGlomerular MesangiumHalf-LifeImmediate-Early ProteinsMolecular Sequence DataPromoter Regions, GeneticProtein Kinase CRatsRNA, MessengerTranscription FactorsTranscription, GeneticConceptsEarly growth response gene-1Transcriptional inductionResponse gene-1Egr-1Gene 1Electrophoretic mobility shift assaysDependent transcriptional inductionDNA-protein interactionsPromoter deletion analysisMobility shift assaysIntracellular biochemical eventsProtein kinase CInduction of mitogenesisMesangial cellsEarly gene expressionExtracellular matrix productionDeletion analysisPK CShift assaysTranscriptional levelAVP stimulationHeterologous promoterConsensus sequenceGene expressionPromoter activity
1993
PDGF-induced Egr-1 expression in rat mesangial cells is mediated through upstream serum response elements
Rupprecht H, Sukhatme V, Lacy J, Sterzel R, Coleman D. PDGF-induced Egr-1 expression in rat mesangial cells is mediated through upstream serum response elements. American Journal Of Physiology 1993, 265: f351-f360. PMID: 8214093, DOI: 10.1152/ajprenal.1993.265.3.f351.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceDNA-Binding ProteinsEarly Growth Response Protein 1Gene ExpressionGlomerular MesangiumImmediate-Early ProteinsMolecular Sequence DataNuclear ProteinsOligonucleotide ProbesPhosphorylationPlatelet-Derived Growth FactorPromoter Regions, GeneticRatsSerum Response FactorTranscription FactorsTranscription, GeneticTyrosineConceptsPlatelet-derived growth factorPDGF stimulationElectrophoretic mobility shift assaysNuclear run-off assaysPromoter deletion analysisEgr-1 transcriptionEarly growth response gene-1Serum response elementUpstream serum response elementsProtein tyrosine phosphorylationMobility shift assaysRun-off assaysResponse gene-1Egr-1 inductionEgr-1 mRNAEgr-1 expressionImmediate early genesTranscriptional inductionDeletion analysisTyrosine phosphorylationShift assaysTranscriptional levelConsensus sequenceTranscription rateGene expression