Live cell imaging of primary rat neonatal cardiomyocytes following adenoviral and lentiviral transduction using confocal spinning disk microscopy.
Sakurai T, Lanahan A, Woolls MJ, Li N, Tirziu D, Murakami M. Live cell imaging of primary rat neonatal cardiomyocytes following adenoviral and lentiviral transduction using confocal spinning disk microscopy. Journal Of Visualized Experiments 2014, e51666. PMID: 24998400, PMCID: PMC4209952, DOI: 10.3791/51666.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsImage Processing, Computer-AssistedLentivirusMicroscopy, ConfocalMyocytes, CardiacRatsTransduction, GeneticConceptsRat neonatal cardiomyocytesConfocal spinning disk microscopyLive cell imagesFunction of genesConfocal spinning disk microscopeNeonatal cardiomyocytesPrimary rat neonatal cardiomyocytesCultured primary cellsUse of siRNAsDisk microscopyDifferent genesChemical modulatorsCellular eventsDisk microscopePrimary cellsLentiviral transductionExpression levelsAdenoviral transductionCell imagingTransductionGenesTransduction ratesMicroscope technologyCellsCardiomyocytes