1996
Use of Codon Cassette Mutagenesis for Saturation Mutagenesis
Kegler-Ebo D, Polack G, DiMaio D. Use of Codon Cassette Mutagenesis for Saturation Mutagenesis. Methods In Molecular Biology 1996, 57: 297-310. PMID: 8850016, DOI: 10.1385/0-89603-332-5:297.Peer-Reviewed Original Research
1994
Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes
Kegler-Ebo D, Docktor C, DiMaio D. Codon cassette mutagenesis: a general method to insert or replace individual codons by using universal mutagenic cassettes. Nucleic Acids Research 1994, 22: 1593-1599. PMID: 8202358, PMCID: PMC308034, DOI: 10.1093/nar/22.9.1593.Peer-Reviewed Original Research
1993
Hierarchy of polyadenylation site usage by bovine papillomavirus in transformed mouse cells
Andrews E, DiMaio D. Hierarchy of polyadenylation site usage by bovine papillomavirus in transformed mouse cells. Journal Of Virology 1993, 67: 7705-7710. PMID: 7901430, PMCID: PMC238246, DOI: 10.1128/jvi.67.12.7705-7710.1993.Peer-Reviewed Original ResearchAnimalsAntisense Elements (Genetics)Base SequenceBovine papillomavirus 1Cell Transformation, ViralCells, CulturedCloning, MolecularConsensus SequenceDNA, ComplementaryMiceMolecular Sequence DataMutagenesisPoly APolymerase Chain ReactionRegulatory Sequences, Nucleic AcidRNA Processing, Post-TranscriptionalRNA, MessengerSequence Analysis, DNA
1992
The putative E5 open reading frame of cottontail rabbit papillomavirus is dispensable for papilloma formation in domestic rabbits
Brandsma J, Yang Z, DiMaio D, Barthold S, Johnson E, Xiao W. The putative E5 open reading frame of cottontail rabbit papillomavirus is dispensable for papilloma formation in domestic rabbits. Journal Of Virology 1992, 66: 6204-6207. PMID: 1326666, PMCID: PMC283673, DOI: 10.1128/jvi.66.10.6204-6207.1992.Peer-Reviewed Original ResearchConceptsE5 ORFCRPV DNACRPV genomeCottontail rabbit papillomavirusE5 mutantsFrameshift mutantsBiological functionsORFRabbit papillomavirusMutantsMutant DNATransition mutationsPapilloma formationDNA preparationsDNABiochemical analysisGenomeE5 regionDomestic rabbitsFrame mutationsMutationsCodonGenesInduction of papillomasFrameshiftA glutamine residue in the membrane-associating domain of the bovine papillomavirus type 1 E5 oncoprotein mediates its binding to a transmembrane component of the vacuolar H(+)-ATPase
Goldstein D, Kulke R, Dimaio D, Schlegel R. A glutamine residue in the membrane-associating domain of the bovine papillomavirus type 1 E5 oncoprotein mediates its binding to a transmembrane component of the vacuolar H(+)-ATPase. Journal Of Virology 1992, 66: 405-413. PMID: 1370089, PMCID: PMC238300, DOI: 10.1128/jvi.66.1.405-413.1992.Peer-Reviewed Original ResearchConceptsK proteinGrowth factor receptorE5 oncoproteinGlutamine residuesRandom hydrophobic sequencesSpecific amino acid residuesMembrane-associated domainMajor transforming proteinK protein componentCarboxyl-terminal domainEndoplasmic reticulum membraneFactor receptorBovine papillomavirus type 1Colony-stimulating factor 1 receptorTransformation-defective mutantsAmino acid residuesPotential binding sitesPlatelet-derived growth factor receptorAmino acid substitutionsPapillomavirus type 1Hydrophilic amino acidsE5 dimerE5 mutantsFactor 1 receptorProtein complexes
1989
Transforming activity of a 16-amino-acid segment of the bovine papillomavirus E5 protein linked to random sequences of hydrophobic amino acids
Horwitz B, Weinstat D, DiMaio D. Transforming activity of a 16-amino-acid segment of the bovine papillomavirus E5 protein linked to random sequences of hydrophobic amino acids. Journal Of Virology 1989, 63: 4515-4519. PMID: 2552136, PMCID: PMC251082, DOI: 10.1128/jvi.63.11.4515-4519.1989.Peer-Reviewed Original ResearchConceptsE5 proteinAmino acidsWild-type E5 proteinBovine papillomavirus E5 proteinAmino acid sequence requirementsHydrophobic amino acid sequenceCarboxyl-terminal amino acidsMouse C127 cellsAmino acid sequenceBovine papillomavirus type 1Carboxyl-terminal portionWild-type onesHydrophobic amino acidsPapillomavirus type 1Hydrophobic sequenceDifferent amino acidsAcid sequenceC127 cellsSequence requirementsE5 geneCell transformationFoci formationSubstitution mutationsCell membraneProteinStructure, Activity, and Regulation of the Bovine Papillomavirus E5 Gene and Its Transforming Protein Product
Horwitz B, Settleman J, Prakash S, DiMaio D. Structure, Activity, and Regulation of the Bovine Papillomavirus E5 Gene and Its Transforming Protein Product. Current Topics In Microbiology And Immunology 1989, 144: 143-151. PMID: 2551579, DOI: 10.1007/978-3-642-74578-2_18.Peer-Reviewed Original ResearchConceptsE5 geneFoci formationBovine papillomavirus type 1 DNACell focus formationBPV geneCodon resultsC127 cellsMouse cellsTumorigenic transformationProtein productsK polypeptideGenesViral mutantsBiochemical analysisEfficient transformationCellsMutantsPolypeptideDNARegulationExpressionDownstreamActivityTranslationFormation
1988
Bovine papillomavirus E2 gene regulates expression of the viral E5 transforming gene
Prakash S, Horwitz B, Zibello T, Settleman J, DiMaio D. Bovine papillomavirus E2 gene regulates expression of the viral E5 transforming gene. Journal Of Virology 1988, 62: 3608-3613. PMID: 2843663, PMCID: PMC253501, DOI: 10.1128/jvi.62.10.3608-3613.1988.Peer-Reviewed Original ResearchAcetyltransferasesAmino Acid SequenceAnimalsBase SequenceBovine papillomavirus 1Cell LineCell Transformation, ViralChloramphenicol O-AcetyltransferaseDNA-Binding ProteinsGene Expression RegulationGenes, ViralMolecular Sequence DataMutationOncogene Proteins, ViralOncogenesPapillomaviridaePlasmidsPrecipitin TestsPromoter Regions, GeneticTransfectionViral Proteins
1986
Translation of open reading frame E5 of bovine papillomavirus is required for its transforming activity.
DiMaio D, Guralski D, Schiller J. Translation of open reading frame E5 of bovine papillomavirus is required for its transforming activity. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 1797-1801. PMID: 3006073, PMCID: PMC323171, DOI: 10.1073/pnas.83.6.1797.Peer-Reviewed Original ResearchConceptsMouse C127 cellsC127 cellsTransformation defectGenetic mapping experimentsCorrect reading frameStrong genetic evidenceBovine papillomavirus type 1Series of mutationsViral DNAPapillomavirus type 1Putative genesGenetic evidenceHydrophobic proteinsReading frameFrameshift mutantsPolypeptide domainsBovine papillomavirus DNAOncogenic transformationDifferent mutantsORFSecond mutationSequence changesMutantsMapping experimentsFrameshift mutation
1983
Identification of two distinct regulatory regions adjacent to the human β-interferon gene
Zinn K, Dimaio D, Maniatis T. Identification of two distinct regulatory regions adjacent to the human β-interferon gene. Cell 1983, 34: 865-879. PMID: 6313211, DOI: 10.1016/0092-8674(83)90544-5.Peer-Reviewed Original ResearchConceptsIFN gene expressionBovine papilloma virusRegulatory regionsExpression of deletion mutantsGene expressionMRNA cap sitePoly(I)-poly(CCell linesStable cell linesIndependent cell linesCloned geneStructural geneDeletion mutantsLevel of expressionKinetics of inductionCap siteMouse cellsHuman beta-interferonGenesInduced expressionConstitutive levelsDeletionBeta-interferonExpressionPapilloma virus
1982
Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells.
DiMaio D, Treisman R, Maniatis T. Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1982, 79: 4030-4034. PMID: 6287462, PMCID: PMC346570, DOI: 10.1073/pnas.79.13.4030.Peer-Reviewed Original ResearchConceptsMammalian cellsBacterial cellsInduce focus formationHuman beta-globin gene clusterBeta-globin gene clusterBPV DNADerivative of pBR322Mouse C127 cellsTransformation of bacteriaTransformed mouse cellsHuman beta-globin geneExtrachromosomal elementsGene clusterBeta-globin geneBovine papillomavirus vectorDeletion derivativesInput DNACellular genesRecombinant plasmidC127 cellsCopy numberMouse cellsTransformed cellsPlasmidFoci formationRegulatory mutants of simian virus 40 Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes
DiMaio D, Nathans D. Regulatory mutants of simian virus 40 Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes. Journal Of Molecular Biology 1982, 156: 531-548. PMID: 6288959, DOI: 10.1016/0022-2836(82)90265-0.Peer-Reviewed Original ResearchConceptsViral DNA replicationDNA replicationEarly gene transcriptionT antigenGene transcriptionGene expressionCold-sensitive defectSecond-site mutationsSegment of DNAT antigen functionLate protein synthesisSimian virus 40 mutantsSV40 early proteinsLarge T antigenT-antigen geneEarly gene expressionBase pair substitutionsRegulatory mutantsRegulatory segmentRegulatory regionsDefined deletionsViral regulatory regionViral genesMutantsDNA show
1981
Directed Mutagenesis
Shortle D, DiMaio D, Nathans D. Directed Mutagenesis. Annual Review Of Genetics 1981, 15: 265-294. PMID: 6279018, DOI: 10.1146/annurev.ge.15.120181.001405.Peer-Reviewed Original ResearchBinding of an SV40 T antigen-related protein to the DNA of SV40 regulatory mutants
McKay R, DiMaio D. Binding of an SV40 T antigen-related protein to the DNA of SV40 regulatory mutants. Nature 1981, 289: 810-813. PMID: 6258090, DOI: 10.1038/289810a0.Peer-Reviewed Original Research
1980
Cold-sensitive regulatory mutants of simian virus 40
DiMaio D, Nathans D. Cold-sensitive regulatory mutants of simian virus 40. Journal Of Molecular Biology 1980, 140: 129-142. PMID: 6251230, DOI: 10.1016/0022-2836(80)90359-9.Peer-Reviewed Original ResearchConceptsRegulatory segmentBase substitutionsBglI siteWild-type plaquesProtein-coding sequencesProtein-nucleic acid interactionsCold-sensitive mutantsOrigin of replicationSet of mutantsSimian virus 40 mutantsDNA polymerase IBase substitution mutationsRNA processingRegulatory mutantsDNA replicationReplication originsViable mutantsSimian virus 40Small plaquesMutational alterationsWild-type virusPolymerase IMutantsRegulatory phenomenaSubstitution mutations