2020
Fc Gamma Receptors and Complement Component 3 Facilitate Anti-fVIII Antibody Formation
Zerra PE, Arthur CM, Chonat S, Maier CL, Mener A, Shin S, Allen JWL, Baldwin WH, Cox C, Verkerke H, Jajosky RP, Tormey CA, Meeks SL, Stowell SR. Fc Gamma Receptors and Complement Component 3 Facilitate Anti-fVIII Antibody Formation. Frontiers In Immunology 2020, 11: 905. PMID: 32582142, PMCID: PMC7295897, DOI: 10.3389/fimmu.2020.00905.Peer-Reviewed Original ResearchConceptsAnti-FVIII antibody formationAnti-FVIII antibodiesKnockout recipientsAntibody formationWT recipientsFc gamma receptorsAnti-FVIII antibody developmentGamma receptorsRole of FcγRsFVIII exposureAntibody levelsWT miceTherapeutic optionsAntibody responseC3 knockoutTarget antigenHemophilia AImmune systemFcγRAntibody developmentInfusionRecipientsAntibodiesPotential mechanismsPatients
2018
Marginal Zone B Cells Induce Alloantibody Formation Following RBC Transfusion
Patel SR, Gibb DR, Girard-Pierce K, Zhou X, Rodrigues LC, Arthur CM, Bennett AL, Jajosky RP, Fuller M, Maier CL, Zerra PE, Chonat S, Smith NH, Tormey CA, Hendrickson JE, Stowell SR. Marginal Zone B Cells Induce Alloantibody Formation Following RBC Transfusion. Frontiers In Immunology 2018, 9: 2516. PMID: 30505302, PMCID: PMC6250814, DOI: 10.3389/fimmu.2018.02516.Peer-Reviewed Original ResearchConceptsMZ B cellsMarginal zone B cellsRBC transfusionB cellsKEL RBCsImmune factorsAntibody formationRBC antigensRed blood cell alloimmunizationKey immune factorsSpecific immune compartmentCD4 T cellsFollicular B cellsAlloantibody formationImmune compartmentAntibody responseT cellsTherapeutic modalitiesTransfusionImmunological challengeImmune pathwaysMarginal sinusSplenic compartmentsImmune constituentsAlloimmunizationTransfused platelets enhance alloimmune responses to transfused KEL-expressing red blood cells in a murine model.
Madrid DJ, Santhanakrishnan M, Liu J, Gibb DR, Liu D, Natarajan P, Beitler D, Shi Z, Mo C, Tormey CA, Patel SR, Stowell SR, Hendrickson JE. Transfused platelets enhance alloimmune responses to transfused KEL-expressing red blood cells in a murine model. Blood Transfusion = Trasfusione Del Sangue 2018, 17: 368-377. PMID: 30418129, PMCID: PMC6774925, DOI: 10.2450/2018.0178-18.Peer-Reviewed Original ResearchConceptsRed blood cell transfusionBlood cell transfusionTransfusion of plateletsAlloimmune responseRed blood cellsCell transfusionWild-type recipientsBlood cellsImmune responseMurine modelRed blood cell alloimmunisationCD40/CD40L interactionHuman KEL glycoproteinWild-type C57BL/6Humoral immune responseAdaptive immune responsesDevelopment of alloantibodiesPlatelet-rich plasmaEndogenous plateletsCD40L interactionRecipient treatmentTransfusionDanger signalsCD40LTransgenic donors
2017
The impact of vaccination on RBC alloimmunization in a murine model
Natarajan P, Santhanakrishnan M, Tormey CA, Hendrickson JE. The impact of vaccination on RBC alloimmunization in a murine model. Vox Sanguinis 2017, 112: 598-600. PMID: 28597520, PMCID: PMC5575953, DOI: 10.1111/vox.12541.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsErythrocyte TransfusionErythrocytesInflammationIsoantibodiesMiceMice, Inbred C57BLModels, AnimalTransplantation ImmunologyVaccinationConceptsImmune responseMurine modelRBC alloantibody formationDamage-associated molecular patternsImpact of vaccinationRBC alloimmunizationAlloantibody formationHepB vaccineRBC transfusionAdaptive immunityAnimal modelsAlloimmunizationMolecular patternsVaccineVaccinationFuture studiesResponseTransfusionImmunity
2016
Antigen modulation as a potential mechanism of anti-KEL immunoprophylaxis in mice
Liu J, Santhanakrishnan M, Natarajan P, Gibb DR, Eisenbarth SC, Tormey CA, Siddon AJ, Stowell SR, Branch DR, Hendrickson JE. Antigen modulation as a potential mechanism of anti-KEL immunoprophylaxis in mice. Blood 2016, 128: 3159-3168. PMID: 27688803, PMCID: PMC5201095, DOI: 10.1182/blood-2016-06-724732.Peer-Reviewed Original ResearchConceptsMechanism of actionKO miceRed blood cell alloimmunizationGlycoprotein antigenMurine RBCsHuman KEL glycoproteinRh immune globulinSingle knockout miceWild-type recipientsImmunoprophylaxis programKO recipientsPrevent alloimmunizationImmune globulinSerious complicationsAntigen modulationMurine modelImmunoprophylaxisFcγ receptorsKEL antigenAlloimmunizationFlow cytometryRapid clearanceWestern blotMiceAntigenClinically significant anti‐KEL RBC alloantibodies are transferred by breast milk in a murine model
Santhanakrishnan M, Tormey CA, Natarajan P, Liu J, Hendrickson JE. Clinically significant anti‐KEL RBC alloantibodies are transferred by breast milk in a murine model. Vox Sanguinis 2016, 111: 79-87. PMID: 26950259, PMCID: PMC4938760, DOI: 10.1111/vox.12387.Peer-Reviewed Original ResearchConceptsBreast milkRBC alloantibodiesIgA antibodiesMurine modelHuman KEL glycoproteinSeries of pregnanciesBreast milk transferHuman breast milkWild-type pupsKEL RBCsNursing infantsClinical significanceAlloantibodiesNursing pupsMurine dataTransfusion experimentsMilk transferInfantsPupsTransfusionHuman dataSequelaePregnancyAnemiaPremature clearance
2015
Riboflavin‐ultraviolet light pathogen reduction treatment does not impact the immunogenicity of murine red blood cells
Tormey CA, Santhanakrishnan M, Smith NH, Liu J, Marschner S, Goodrich RP, Hendrickson JE. Riboflavin‐ultraviolet light pathogen reduction treatment does not impact the immunogenicity of murine red blood cells. Transfusion 2015, 56: 863-872. PMID: 26643781, DOI: 10.1111/trf.13432.Peer-Reviewed Original ResearchConceptsWhite blood cellsFresh whole bloodPathogen reduction treatmentAlloimmune responseWhole bloodRBC antigensSyngeneic RBCsNegative direct antiglobulin testBlood cellsRBC antigen expressionRecipient alloimmune responsesDirect antiglobulin testMurine red blood cellsPathogen inactivation treatmentPosttransfusion RBC recoveryKEL RBCsRBC alloimmunizationImmunogenic neoantigensRed blood cellsAntiglobulin testBlood donorsImmune responseAntigen expressionHours posttransfusionHuman antigens
2011
Rapid clearance of transfused murine red blood cells is associated with recipient cytokine storm and enhanced alloimmunogenicity
Hendrickson JE, Hod EA, Cadwell CM, Eisenbarth SC, Spiegel DA, Tormey CA, Spitalnik SL, Zimring JC. Rapid clearance of transfused murine red blood cells is associated with recipient cytokine storm and enhanced alloimmunogenicity. Transfusion 2011, 51: 2445-2454. PMID: 21569043, PMCID: PMC3175302, DOI: 10.1111/j.1537-2995.2011.03162.x.Peer-Reviewed Original ResearchConceptsProinflammatory cytokine stormRed blood cellsHeat-treated red blood cellsFresh red blood cellsCytokine stormRBC clearanceAntigen expressionRapid clearanceBlood cellsAdverse transfusion outcomesFlow cytometric crossmatchChemoattractant protein-1Control red blood cellsDonor red blood cellsMurine red blood cellsStored red blood cellsTransgenic antigensCytometric crossmatchCytokine responsesRecipient miceInterleukin-6Transfusion outcomesHours posttransfusionHeat treatmentFourteen days