2016
Antigen modulation as a potential mechanism of anti-KEL immunoprophylaxis in mice
Liu J, Santhanakrishnan M, Natarajan P, Gibb DR, Eisenbarth SC, Tormey CA, Siddon AJ, Stowell SR, Branch DR, Hendrickson JE. Antigen modulation as a potential mechanism of anti-KEL immunoprophylaxis in mice. Blood 2016, 128: 3159-3168. PMID: 27688803, PMCID: PMC5201095, DOI: 10.1182/blood-2016-06-724732.Peer-Reviewed Original ResearchConceptsMechanism of actionKO miceRed blood cell alloimmunizationGlycoprotein antigenMurine RBCsHuman KEL glycoproteinRh immune globulinSingle knockout miceWild-type recipientsImmunoprophylaxis programKO recipientsPrevent alloimmunizationImmune globulinSerious complicationsAntigen modulationMurine modelImmunoprophylaxisFcγ receptorsKEL antigenAlloimmunizationFlow cytometryRapid clearanceWestern blotMiceAntigen
2011
Rapid clearance of transfused murine red blood cells is associated with recipient cytokine storm and enhanced alloimmunogenicity
Hendrickson JE, Hod EA, Cadwell CM, Eisenbarth SC, Spiegel DA, Tormey CA, Spitalnik SL, Zimring JC. Rapid clearance of transfused murine red blood cells is associated with recipient cytokine storm and enhanced alloimmunogenicity. Transfusion 2011, 51: 2445-2454. PMID: 21569043, PMCID: PMC3175302, DOI: 10.1111/j.1537-2995.2011.03162.x.Peer-Reviewed Original ResearchConceptsProinflammatory cytokine stormRed blood cellsHeat-treated red blood cellsFresh red blood cellsCytokine stormRBC clearanceAntigen expressionRapid clearanceBlood cellsAdverse transfusion outcomesFlow cytometric crossmatchChemoattractant protein-1Control red blood cellsDonor red blood cellsMurine red blood cellsStored red blood cellsTransgenic antigensCytometric crossmatchCytokine responsesRecipient miceInterleukin-6Transfusion outcomesHours posttransfusionHeat treatmentFourteen days