2000
Determinants of NPC1 Expression and Action: Key Promoter Regions, Posttranscriptional Control, and the Importance of a “Cysteine-Rich” Loop
Watari H, Blanchette-Mackie E, Dwyer N, Watari M, Burd C, Patel S, Pentchev P, Strauss J. Determinants of NPC1 Expression and Action: Key Promoter Regions, Posttranscriptional Control, and the Importance of a “Cysteine-Rich” Loop. Experimental Cell Research 2000, 259: 247-256. PMID: 10942596, DOI: 10.1006/excr.2000.4976.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarcinoma, HepatocellularCarrier ProteinsCHO CellsCricetinaeCysteineGene Expression RegulationHumansIntracellular Signaling Peptides and ProteinsLysosomesMembrane GlycoproteinsNiemann-Pick C1 ProteinNiemann-Pick DiseasesPlasmidsProgesteronePromoter Regions, GeneticProtein Structure, TertiaryProteinsRNA Processing, Post-TranscriptionalRNA, MessengerTransfectionTumor Cells, CulturedZincConceptsNiemann-Pick type C diseaseCholesterol trafficking defectType C diseaseC diseaseNPC1 expressionTrafficking defectsNPC1 genePromoter activityMajor transcription initiation siteBase pairsLate endosomal compartmentsTranscription initiation siteTranscription start siteI1061T mutationKey promoter regionChinese hamster cell lineProgesterone-treated cellsHamster cell linesNPC1 mRNA levelsProgesterone-induced increaseZinc-binding activityProtein synthesis inhibitorCT60 cellsThreonine residuesPosttranscriptional regulation
1998
Novel pathways, membrane coats and PI kinase regulation in yeast lysosomal trafficking
Burd C, Babst M, Emr S. Novel pathways, membrane coats and PI kinase regulation in yeast lysosomal trafficking. Seminars In Cell And Developmental Biology 1998, 9: 527-533. PMID: 9835640, DOI: 10.1006/scdb.1998.0255.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Protein Complex alpha SubunitsAdaptor Proteins, Vesicular TransportAlkaline PhosphataseBiological TransportCarboxypeptidasesCarrier ProteinsCathepsin ACell MembraneCoated Pits, Cell-MembraneFungal ProteinsIntracellular MembranesLysosomesMembrane ProteinsModels, BiologicalMonomeric Clathrin Assembly ProteinsPhosphatidylinositol 3-KinasesSaccharomyces cerevisiaeVacuolesConceptsSorting pathwaysVesicle docking/fusionAdaptor protein-3 complexProtein sorting signalsDocking/fusionPhosphorylation-dependent ubiquitinationVesicle coat proteinsYeast Saccharomyces cerevisiaeMembrane fusion reactionGolgi recyclingEndocytic cargoFYVE domainSorting signalsKinase regulationEukaryotic cellsImportant mechanistic insightsMembrane transport reactionsRecycling pathwaySaccharomyces cerevisiaeBiosynthetic pathwayCoat proteinRecognition motifMembrane coatMolecular mechanismsLysosomal traffickingPhosphatidylinositol(3)-Phosphate Signaling Mediated by Specific Binding to RING FYVE Domains
Burd C, Emr S. Phosphatidylinositol(3)-Phosphate Signaling Mediated by Specific Binding to RING FYVE Domains. Molecular Cell 1998, 2: 157-162. PMID: 9702203, DOI: 10.1016/s1097-2765(00)80125-2.Peer-Reviewed Original ResearchMeSH KeywordsBinding SitesBiological TransportCell CompartmentationFungal ProteinsGenes, ReporterGreen Fluorescent ProteinsLiposomesLuminescent ProteinsLysosomesPhosphatidylinositol 3-KinasesPhosphatidylinositol PhosphatesPhosphorylationProtein BindingRecombinant Fusion ProteinsSaccharomyces cerevisiaeSignal TransductionVacuolesZinc FingersConceptsFYVE domain-containing proteinsLysosomal protein traffickingDomain-containing proteinsGFP reporter proteinDomain fusion proteinLipid-binding assaysReceptor signaling cascadesFYVE fingerFYVE domainMembrane traffickingProtein traffickingPhosphate signalingInositol ringProtein domainsReporter proteinRING domainDownstream effectorsEndocytic compartmentsSignaling cascadesMolecular linkFusion proteinIntracellular localizationVps34Important regulatorRecruit/