1994
The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins.
Görlach M, Burd C, Dreyfuss G. The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins. Journal Of Biological Chemistry 1994, 269: 23074-23078. PMID: 8083209, DOI: 10.1016/s0021-9258(17)31621-6.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceBase SequenceBinding SitesElectrophoresis, Polyacrylamide GelGene AmplificationHeterogeneous-Nuclear Ribonucleoprotein Group CHeterogeneous-Nuclear RibonucleoproteinsMolecular Sequence DataRibonucleoproteinsRNA, Heterogeneous NuclearRNA-Binding ProteinsSequence Homology, Amino AcidConceptsRNA-binding domainRNA-binding specificityHnRNP C1Selection/amplificationC proteinMinimal RNA-binding domainHeterogeneous nuclear ribonucleoprotein C proteinsHnRNP C proteinsRandom sequence RNARNA-binding propertiesDeterminant of RNAFilter binding assaysRNP motifGeneral RNASpecificity residesC1-binding siteSequence RNAMotif familyRNA ligandsU residuesContiguous stretchesRNAAmino acidsProteinVariable regionsThe mRNA Poly(A)-Binding Protein: Localization, Abundance, and RNA-Binding Specificity
Görlach M, Burd C, Dreyfuss G. The mRNA Poly(A)-Binding Protein: Localization, Abundance, and RNA-Binding Specificity. Experimental Cell Research 1994, 211: 400-407. PMID: 7908267, DOI: 10.1006/excr.1994.1104.Peer-Reviewed Original ResearchConceptsHuman PABPRNA binding specificityQuantitative immunoblotting experimentsMost eukaryotic mRNAsRNA-binding propertiesTranslation of mRNAsConfocal immunofluorescence microscopySelection/amplificationEukaryotic mRNAsOligonucleotide poolRNA sequencesRich sequencesCellular localizationBinding proteinHeLa cellsImmunofluorescence microscopyImmunoblotting experimentsLow turnover rateLow affinityPABPProteinAbundanceMRNAIntracellular concentrationTurnover rateRNA binding specificity of hnRNP A1: significance of hnRNP A1 high‐affinity binding sites in pre‐mRNA splicing.
Burd C, Dreyfuss G. RNA binding specificity of hnRNP A1: significance of hnRNP A1 high‐affinity binding sites in pre‐mRNA splicing. The EMBO Journal 1994, 13: 1197-1204. PMID: 7510636, PMCID: PMC394929, DOI: 10.1002/j.1460-2075.1994.tb06369.x.Peer-Reviewed Original ResearchAnimalsBase SequenceBinding SitesCell NucleusConsensus SequenceDNA PrimersGlobinsHeterogeneous Nuclear Ribonucleoprotein A1Heterogeneous-Nuclear Ribonucleoprotein Group A-BHeterogeneous-Nuclear RibonucleoproteinsIntronsMolecular Sequence DataOligodeoxyribonucleotidesOligoribonucleotidesRecombinant ProteinsRibonucleoproteinsRNARNA PrecursorsRNA SplicingRNA-Binding ProteinsUltraviolet Rays
1989
Primary structures of the heterogeneous nuclear ribonucleoprotein A2, B1, and C2 proteins: a diversity of RNA binding proteins is generated by small peptide inserts.
Burd C, Swanson M, Görlach M, Dreyfuss G. Primary structures of the heterogeneous nuclear ribonucleoprotein A2, B1, and C2 proteins: a diversity of RNA binding proteins is generated by small peptide inserts. Proceedings Of The National Academy Of Sciences Of The United States Of America 1989, 86: 9788-9792. PMID: 2557628, PMCID: PMC298587, DOI: 10.1073/pnas.86.24.9788.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCloning, MolecularDNA Transposable ElementsDNA, NeoplasmElectrophoresis, Gel, Two-DimensionalHeLa CellsHeterogeneous Nuclear Ribonucleoprotein A1Heterogeneous-Nuclear Ribonucleoprotein Group A-BHeterogeneous-Nuclear Ribonucleoprotein Group CHeterogeneous-Nuclear RibonucleoproteinsHumansMolecular Sequence DataProtein BiosynthesisRestriction MappingRibonucleoproteinsRNA, Heterogeneous NuclearSequence Homology, Nucleic AcidSoftwareTransfectionConceptsAmino acid sequenceCS-RBDsHeterogeneous nuclear ribonucleoprotein A2C2 proteinAmino acidsAcid sequenceDiversity of RNAHnRNP protein A1Amino acid identityAuxiliary domainHnRNP proteinsMRNA splicingProtein cDNAAcid identityCarboxyl terminusAmino terminusPrimary structureProtein A1Frame insertsB1 proteinCDNALarge familyProteinRNATerminus