2005
Proton sensitivity of ASIC1 appeared with the rise of fishes by changes of residues in the region that follows TM1 in the ectodomain of the channel
Coric T, Zheng D, Gerstein M, Canessa CM. Proton sensitivity of ASIC1 appeared with the rise of fishes by changes of residues in the region that follows TM1 in the ectodomain of the channel. The Journal Of Physiology 2005, 568: 725-735. PMID: 16002453, PMCID: PMC1464184, DOI: 10.1113/jphysiol.2005.087734.Peer-Reviewed Original ResearchMeSH KeywordsAcid Sensing Ion ChannelsAmino Acid SequenceAmino AcidsAnimalsEvolution, MolecularFishesHydrogen-Ion ConcentrationIon Channel GatingMembrane ProteinsMolecular Sequence DataNerve Tissue ProteinsProtein Structure, TertiaryProtonsSequence Homology, Amino AcidSodium ChannelsSpecies SpecificityStructure-Activity RelationshipConceptsAmino acid conservationChanges of residuesProton sensitivityMammalian nervous systemChordate lineageEarly vertebratesFunctional chimerasMammalian counterpartsLower vertebratesAllosteric changesProtein sequencesExtracellular domainSequence analysisDifferent speciesRat sequenceRat channelKinetics of activationStructural determinantsDistinct kineticsVertebratesASIC1 channelsMembrane potentialChimerasSharksEctodomain
1998
Structure and function of the Mec-ENaC family of ion channels.
Fyfe G, Quinn A, Canessa C. Structure and function of the Mec-ENaC family of ion channels. Seminars In Nephrology 1998, 18: 138-51. PMID: 9541269.Peer-Reviewed Original ResearchMeSH KeywordsEpithelial Sodium ChannelsEvolution, MolecularHumansIon Channel GatingSodium ChannelsStructure-Activity RelationshipConceptsIon channelsLittle amino acid identityCell type-dependent expressionAmino acid identityAcid identityDistinct tissuesEpithelial sodium channelFunction mutationsRecent discoveryProteinDifferential sensitivitySodium channelsSpecialized neuronsNew familyNociceptive painBlood pressureFamilyTissue injurySodium homeostasisSame common structureCommon ailmentsMechanical stimuliCloningTransductionDiuretic amiloride
1994
Membrane topology of the epithelial sodium channel in intact cells
Canessa C, Merillat A, Rossier B. Membrane topology of the epithelial sodium channel in intact cells. American Journal Of Physiology 1994, 267: c1682-c1690. PMID: 7810611, DOI: 10.1152/ajpcell.1994.267.6.c1682.Peer-Reviewed Original ResearchMeSH KeywordsAmilorideAnimalsCell MembraneCytoskeletonDogsGlycosylationMutagenesis, Site-DirectedRabbitsSodium ChannelsStructure-Activity RelationshipXenopus laevisConceptsLarge hydrophilic loopHydrophilic loopIntact cellsMembrane topologyEpithelial sodium channelPutative transmembrane domainsStop-transfer signalAmiloride-sensitive epithelial sodium channelCell-free translation assaysShort NH2Transmembrane domainMembrane insertionHomologous subunitsXenopus laevis oocytesTranslation assaysSodium channelsGlycosylation sitesCOOH terminusCytoplasmic sideFunctional expressionTerminal endSubunitsHydrophilic NH2Laevis oocytesAlpha-rENaCEpithelial sodium channels
Rossier B, Canessa C, Schild L, Horisberger J. Epithelial sodium channels. Current Opinion In Nephrology & Hypertension 1994, 3: 487-496. PMID: 7804746, DOI: 10.1097/00041552-199409000-00003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiophysical PhenomenaBiophysicsColonEpitheliumHumansKidneyLungSodium ChannelsStructure-Activity RelationshipConceptsEpithelial sodium channelAmiloride-sensitive epithelial sodium channelSodium channelsNovel genesHeteromultimeric proteinsHomologous subunitsDistinct functionsRat epithelial sodium channelPrimary structureCation channelsBiophysical propertiesCritical roleTaste transductionTransductionGenesMechanotransductionSubunitsProteinCell distributionRegulation