Evaluation of Multidimensional Chromatography Coupled with Tandem Mass Spectrometry (LC/LC−MS/MS) for Large-Scale Protein Analysis: The Yeast Proteome
Peng J, Elias J, Thoreen C, Licklider L, Gygi S. Evaluation of Multidimensional Chromatography Coupled with Tandem Mass Spectrometry (LC/LC−MS/MS) for Large-Scale Protein Analysis: The Yeast Proteome. Journal Of Proteome Research 2002, 2: 43-50. PMID: 12643542, DOI: 10.1021/pr025556v.Peer-Reviewed Original ResearchConceptsStrong cation exchangeTandem mass spectrometryMass spectrometryMS/MS spectraComplex protein mixturesReversed-phase chromatographyTwo-dimensional separationMS/MS analysisMultidimensional chromatographyMS/MSMS spectraFraction collectionCation exchangeProtein mixturesSCX chromatographyRP-LCLiquid chromatographyMS analysisChromatographyPeptide identificationSEQUEST algorithmSpectrometryYeast protein databaseSingle analysisLarge-scale protein analysis