2011
Genetic evidence of the regulatory role of parathyroid hormone–related protein in articular chondrocyte maintenance in an experimental mouse model
Macica C, Liang G, Nasiri A, Broadus AE. Genetic evidence of the regulatory role of parathyroid hormone–related protein in articular chondrocyte maintenance in an experimental mouse model. Arthritis & Rheumatism 2011, 63: 3333-3343. PMID: 21702022, PMCID: PMC3197958, DOI: 10.1002/art.30515.Peer-Reviewed Original ResearchConceptsHormone-related proteinDegenerative changesKO miceParathyroid hormone-related proteinMale KO miceExperimental mouse modelMouse articular cartilageTotal histologic scoreIndian hedgehogTibial articular surfaceArticular chondrocytesArticular cartilageGrowth differentiation factor 5Degenerative findingsControl miceHistologic scoresMouse modelCompensatory increaseTime-course studyDifferentiation factor 5PTHrPRegulatory roleMiceConditional deletionChondrocyte maintenance
2008
Regulation of articular chondrocyte proliferation and differentiation by indian hedgehog and parathyroid hormone–related protein in mice
Chen X, Macica CM, Nasiri A, Broadus AE. Regulation of articular chondrocyte proliferation and differentiation by indian hedgehog and parathyroid hormone–related protein in mice. Arthritis & Rheumatism 2008, 58: 3788-3797. PMID: 19035497, PMCID: PMC2599803, DOI: 10.1002/art.23985.Peer-Reviewed Original ResearchConceptsPTHrP expressionParathyroid hormone-related proteinArticular cartilagePTHrP-knockout micePathogenesis of arthritisWeight-bearing jointsHormone-related proteinChondrocyte proliferationIndian hedgehogGrowth plate structureCell of originArticular chondrocytesEpiphyseal growth zoneSecondary ossification centerArticular chondrocyte differentiationArticular chondrocyte proliferationAdult miceAxis participatesKnockin miceMicePTHrPProtein axisOssification centersIhhCartilage
2007
Mechanical regulation of PTHrP expression in entheses
Chen X, Macica C, Nasiri A, Judex S, Broadus AE. Mechanical regulation of PTHrP expression in entheses. Bone 2007, 41: 752-759. PMID: 17869201, PMCID: PMC2219696, DOI: 10.1016/j.bone.2007.07.020.Peer-Reviewed Original ResearchConceptsMedial collateral ligamentPTHrP expressionSurgical transectionBone cell populationsTail suspensionType 1 PTH/PTHrP receptorCell populationsPTH/PTHrP receptorDetailed histological evaluationSitu hybridization histochemical techniquesDistinct temporospatial patternLigament insertion sitesMetaphyseal periosteumPTHrP receptorOsteoclastic activityCollateral ligamentHistological evaluationReporter miceKnockin miceInsertion siteKnockin reporter micePTHrPPTHrP geneCD-1Periosteum
2005
Stretch‐Induced PTH‐Related Protein Gene Expression in Osteoblasts*
Chen X, Macica CM, Ng KW, Broadus AE. Stretch‐Induced PTH‐Related Protein Gene Expression in Osteoblasts*. Journal Of Bone And Mineral Research 2005, 20: 1454-1461. PMID: 16007342, DOI: 10.1359/jbmr.2005.20.8.1454.Peer-Reviewed Original ResearchConceptsAnabolic effectsStretch-activated potassium channelsCandidate mediatorsCyclical stretchPotassium channelsL-type calcium channelsN-terminal PTHPTHrP mRNA expressionStretch-activated cation channelsSmooth muscle cellsOsteoblast-like cellsUMR 201PTHrP gene expressionHypotonic solutionEndogenous mediatorsPTHrP mRNAIntracellular acidosisExtracellular calciumCalcium channelsFlexercell apparatusPTHrPRNase protection assaysMRNA expressionMuscle cellsSkeletal mass