Lentiviral vector-mediated transduction of neural progenitor cells before implantation into injured spinal cord and brain to detect their migration, deliver neurotrophic factors and repair tissue.
Blits B, Kitay BM, Farahvar A, Caperton CV, Dietrich WD, Bunge MB. Lentiviral vector-mediated transduction of neural progenitor cells before implantation into injured spinal cord and brain to detect their migration, deliver neurotrophic factors and repair tissue. Restorative Neurology And Neuroscience 2005, 23: 313-24. PMID: 16477093.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrain InjuriesCell MovementCells, CulturedDisease Models, AnimalEmbryo, MammalianFemaleGenetic VectorsGlial Fibrillary Acidic ProteinGreen Fluorescent ProteinsImmunohistochemistryIntermediate Filament ProteinsLentivirusNerve Growth FactorsNerve Tissue ProteinsNestinNeuronsRatsRats, Inbred F344Rats, Sprague-DawleySpinal Cord InjuriesStem Cell TransplantationStem CellsTransduction, GeneticTubulinConceptsNeural progenitor cellsSpinal cordNeurotrophic factorPositive cellsGFP-positive cellsContused spinal cordProgenitor cellsSpinal cord lesionsCortical neural progenitor cellsLong-term transgene expressionLentiviral vectorsRat spinal cordLentiviral vector-mediated transductionCord lesionsGreen fluorescent proteinReplacement therapyCell replacement therapyVector-mediated transductionNPC survivalLesion siteAstrocytic phenotypeImmune rejectionCordNeurological disordersNon-transduced cells