2013
Effect of a Matrigel Sandwich on Endodermal Differentiation of Human Embryonic Stem Cells
Lawton BR, Sosa JA, Roman S, Krause DS. Effect of a Matrigel Sandwich on Endodermal Differentiation of Human Embryonic Stem Cells. Stem Cell Reviews And Reports 2013, 9: 578-585. PMID: 23719997, DOI: 10.1007/s12015-013-9447-2.Peer-Reviewed Original ResearchMeSH KeywordsCell Culture TechniquesCell DifferentiationCell LineCell LineageCell MovementCell SurvivalCollagenDrug CombinationsEmbryonic Stem CellsEndodermFluorescent Antibody TechniqueGene Expression Regulation, DevelopmentalGlutamine-Fructose-6-Phosphate Transaminase (Isomerizing)Hepatocyte Nuclear Factor 3-alphaHepatocyte Nuclear Factor 3-betaHumansLamininProteoglycansReceptors, Cell SurfaceReverse Transcriptase Polymerase Chain ReactionSOXF Transcription FactorsTime FactorsConceptsHuman embryonic stem cellsEmbryonic stem cellsDefinitive endodermEndodermal differentiationGene expression patternsStem cellsPrecardiac mesodermExpression patternsLow-serum mediumCell deathMesenchymal transitionMigratory characteristicsKey eventsEndodermDifferentiationCell viabilityActivin ASerum mediumCellsGastrulationImproved protocolMesodermInductionGenesNutrients
2007
Ancient and continuing Darwinian selection on insulin-like growth factor II in placental fishes
O'Neill MJ, Lawton BR, Mateos M, Carone DM, Ferreri GC, Hrbek T, Meredith RW, Reznick DN, O'Neill RJ. Ancient and continuing Darwinian selection on insulin-like growth factor II in placental fishes. Proceedings Of The National Academy Of Sciences Of The United States Of America 2007, 104: 12404-12409. PMID: 17636118, PMCID: PMC1941482, DOI: 10.1073/pnas.0705048104.Peer-Reviewed Original ResearchConceptsDarwinian selectionGenetic conflictPositive selectionComparative evolutionary analysisPositive Darwinian selectionNeutral mutation rateInsulin-like growth factor II geneLevel of phenotypeEvolution of placentationGrowth factor II geneRed Queen raceLevel of genesParent-offspring conflictFactor II genePlacental fishGene evolutionLivebearing fishOrder CyprinodontiformesAncient lineageGenomic imprintingStrong selectionEvolutionary analysisPlacental speciesAncient originNatural selection