2013
Effect of a Matrigel Sandwich on Endodermal Differentiation of Human Embryonic Stem Cells
Lawton BR, Sosa JA, Roman S, Krause DS. Effect of a Matrigel Sandwich on Endodermal Differentiation of Human Embryonic Stem Cells. Stem Cell Reviews And Reports 2013, 9: 578-585. PMID: 23719997, DOI: 10.1007/s12015-013-9447-2.Peer-Reviewed Original ResearchMeSH KeywordsCell Culture TechniquesCell DifferentiationCell LineCell LineageCell MovementCell SurvivalCollagenDrug CombinationsEmbryonic Stem CellsEndodermFluorescent Antibody TechniqueGene Expression Regulation, DevelopmentalGlutamine-Fructose-6-Phosphate Transaminase (Isomerizing)Hepatocyte Nuclear Factor 3-alphaHepatocyte Nuclear Factor 3-betaHumansLamininProteoglycansReceptors, Cell SurfaceReverse Transcriptase Polymerase Chain ReactionSOXF Transcription FactorsTime FactorsConceptsHuman embryonic stem cellsEmbryonic stem cellsDefinitive endodermEndodermal differentiationGene expression patternsStem cellsPrecardiac mesodermExpression patternsLow-serum mediumCell deathMesenchymal transitionMigratory characteristicsKey eventsEndodermDifferentiationCell viabilityActivin ASerum mediumCellsGastrulationImproved protocolMesodermInductionGenesNutrients
2007
Physical mapping of the IGF2 locus in the South American opossum Monodelphis domestica
Lawton BR, Obergfell C, O’Neill R, O’Neill M. Physical mapping of the IGF2 locus in the South American opossum Monodelphis domestica. Cytogenetic And Genome Research 2007, 116: 130-131. PMID: 17268191, DOI: 10.1159/000097431.Peer-Reviewed Original ResearchConceptsSouth American opossum Monodelphis domesticaGenomic imprintingLarge-scale genome sequencing effortsOpossum Monodelphis domesticaGenome sequencing effortsInsulin-like growth factor II geneMonodelphis domesticaGrowth factor II geneFactor II geneGene functionModel organismsGene regulationSequencing effortsPhysical mappingIGF2 locusRegulatory mechanismsM. domesticaMarsupialsDomesticaImprintingRegulationGenesLociOrganismsMechanism
2005
Allelic expression of IGF2 in live-bearing, matrotrophic fishes
Lawton BR, Sevigny L, Obergfell C, Reznick D, O’Neill R, O’Neill M. Allelic expression of IGF2 in live-bearing, matrotrophic fishes. Development Genes And Evolution 2005, 215: 207-212. PMID: 15654625, DOI: 10.1007/s00427-004-0463-8.Peer-Reviewed Original ResearchConceptsInsulin-like growth factor 2Parent-specific gene expressionGenomic imprinting predictsGrowth regulatory genesMatrotrophic fishPoeciliopsis prolificaTranscriptional silencingHeterandria formosaRegulatory genesFish speciesAllelic expressionEmbryonic developmentIGF2 activityGene expressionPlacental animalsDevelopmental expressionGrowth factor 2GenesParental investmentEmbryonic growthSpeciesFactor 2ExpressionVertebratesEutherians