2021
Single-molecule manipulation of macromolecules on GUV or SUV membranes using optical tweezers
Wang Y, Kumar A, Jin H, Zhang Y. Single-molecule manipulation of macromolecules on GUV or SUV membranes using optical tweezers. Biophysical Journal 2021, 120: 5454-5465. PMID: 34813728, PMCID: PMC8715244, DOI: 10.1016/j.bpj.2021.11.2884.Peer-Reviewed Original ResearchConceptsOptical tweezersOptical trappingSingle-molecule manipulation studiesMembrane protein dynamicsMembrane proteinsSingle-molecule manipulationMembrane tensionTweezersHigh membrane tensionProtein conformational changesModel membranesMembrane bindingProtein dynamicsC2AB domainConformational changesManipulation studiesSUV membranesReversible foldingSmall unilamellar vesiclesGUVsTrappingDNA hairpinsUnilamellar vesiclesProteinMembrane
2018
Nanoscale virtual potentials using optical tweezers
Kumar A, Bechhoefer J. Nanoscale virtual potentials using optical tweezers. Applied Physics Letters 2018, 113: 183702. DOI: 10.1063/1.5055580.Peer-Reviewed Original ResearchDouble-well potentialOptical tweezersOptical tweezers techniqueSpatial light modulatorAdjustable barrier heightOptical trapVirtual potentialFeedback trapLight modulatorTrap centersHarmonic potentialIsotropic trapDual beamBarrier heightParticle positionsArbitrary potentialTweezersColloidal particlesLength scalesTrapsWell separationParticlesBeamModulatorPotentialOptical feedback tweezers
Kumar A, Bechhoefer J. Optical feedback tweezers. 2018, 10723: 107232j. DOI: 10.1117/12.2323837.Peer-Reviewed Original ResearchFeedback trapOptical trapAcoustooptic deflectorTime-shared optical tweezersDouble-well potentialVirtual potentialOptical tweezersLaser powerPosition detectorTrap positionBarrier heightParticle positionsArbitrary potentialTweezersObserved fluctuationsTrapsParticlesWell separationIndependent controlDeflectorLoop rateDetectorPreliminary experimentsHydrodynamic forcesFeedback force