Single-molecule manipulation of macromolecules on GUV or SUV membranes using optical tweezers
Wang Y, Kumar A, Jin H, Zhang Y. Single-molecule manipulation of macromolecules on GUV or SUV membranes using optical tweezers. Biophysical Journal 2021, 120: 5454-5465. PMID: 34813728, PMCID: PMC8715244, DOI: 10.1016/j.bpj.2021.11.2884.Peer-Reviewed Original ResearchConceptsOptical tweezersOptical trappingSingle-molecule manipulation studiesMembrane protein dynamicsMembrane proteinsSingle-molecule manipulationMembrane tensionTweezersHigh membrane tensionProtein conformational changesModel membranesMembrane bindingProtein dynamicsC2AB domainConformational changesManipulation studiesSUV membranesReversible foldingSmall unilamellar vesiclesGUVsTrappingDNA hairpinsUnilamellar vesiclesProteinMembrane