2013
Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition
Lee MT, Bonneau AR, Takacs CM, Bazzini AA, DiVito KR, Fleming ES, Giraldez AJ. Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition. Nature 2013, 503: 360-364. PMID: 24056933, PMCID: PMC3925760, DOI: 10.1038/nature12632.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCellular ReprogrammingEmbryonic DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalHomeodomain ProteinsMicroRNAsMothersNanog Homeobox ProteinOctamer Transcription Factor-3Pluripotent Stem CellsRibosomesSOXB1 Transcription FactorsTranscriptomeZebrafishZebrafish ProteinsZygoteConceptsZygotic genome activationZygotic transitionMiR-430Zygotic gene expressionZygotic gene activationZygotic developmental programMaternal messenger RNAsZygotic programGenome activationZygotic genesZygotic transcriptionRibosome profilingDevelopmental programGene activationTranscription factorsDevelopmental arrestGene expressionSoxB1POU5F1Maternal programMessenger RNANanogGastrulationZebrafishGenes
2012
Ribosome Profiling Shows That miR-430 Reduces Translation Before Causing mRNA Decay in Zebrafish
Bazzini AA, Lee MT, Giraldez AJ. Ribosome Profiling Shows That miR-430 Reduces Translation Before Causing mRNA Decay in Zebrafish. Science 2012, 336: 233-237. PMID: 22422859, PMCID: PMC3547538, DOI: 10.1126/science.1215704.Peer-Reviewed Original ResearchConceptsMiR-430MRNA decayTranslational repressionMutant zebrafish embryosMessenger RNA decayNumber of ribosomesRibosome occupancyZebrafish developmentRibosome profilingTarget repressionRNA decayTranslation initiationZebrafish embryosTarget mRNAsRibosome densityEndogenous mRNAGene expressionPolyadenylate tailDeadenylationRepressionMRNAZebrafishRibosomesMicroRNAsRate of initiation
2010
A Novel miRNA Processing Pathway Independent of Dicer Requires Argonaute2 Catalytic Activity
Cifuentes D, Xue H, Taylor DW, Patnode H, Mishima Y, Cheloufi S, Ma E, Mane S, Hannon GJ, Lawson ND, Wolfe SA, Giraldez AJ. A Novel miRNA Processing Pathway Independent of Dicer Requires Argonaute2 Catalytic Activity. Science 2010, 328: 1694-1698. PMID: 20448148, PMCID: PMC3093307, DOI: 10.1126/science.1190809.Peer-Reviewed Original ResearchAnimalsArgonaute ProteinsBiocatalysisEmbryo, NonmammalianEmbryonic DevelopmentErythropoiesisEukaryotic Initiation Factor-2HumansMicroRNAsModels, BiologicalMorphogenesisNucleic Acid ConformationRecombinant ProteinsRibonuclease IIIRNA PrecursorsRNA Processing, Post-TranscriptionalZebrafishZebrafish Proteins
2006
Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs
Giraldez AJ, Mishima Y, Rihel J, Grocock RJ, Van Dongen S, Inoue K, Enright AJ, Schier AF. Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs. Science 2006, 312: 75-79. PMID: 16484454, DOI: 10.1126/science.1122689.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAdenosineAnimalsEmbryo, NonmammalianEmbryonic DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGenes, ReporterGreen Fluorescent ProteinsMicroRNAsMutationOligonucleotide Array Sequence AnalysisPolymersProtein BiosynthesisRibonuclease IIIRNA StabilityRNA, MessengerTranscription, GeneticUp-RegulationZebrafishZygoteConceptsMessenger RNA moleculesTarget messenger RNA moleculesMiR-430Vivo target validationVertebrate genesZygotic transcriptionEarly embryogenesisRNA moleculesMicroarray approachVivo functionDeadenylationTarget validationEarly developmentMechanism of actionMost targetsEmbryogenesisMorphogenesisTranscriptionGenesMicroRNAsTarget
2005
MicroRNAs Regulate Brain Morphogenesis in Zebrafish
Giraldez AJ, Cinalli RM, Glasner ME, Enright AJ, Thomson JM, Baskerville S, Hammond SM, Bartel DP, Schier AF. MicroRNAs Regulate Brain Morphogenesis in Zebrafish. Science 2005, 308: 833-838. PMID: 15774722, DOI: 10.1126/science.1109020.Peer-Reviewed Original ResearchConceptsRNA-binding domainMultiple cell typesDicer mutantsMutant embryosSmall RNAsPrecursor miRNAsAxis formationMature miRNAsRibonuclease IIIHeart developmentGene expressionBrain morphogenesisAbnormal morphogenesisLater stepsMiRNA formationMorphogenesisMutantsMiRNAsCell typesBrain formationEssential roleZebrafishBrain defectsSomitogenesisGastrulation