2024
Maternal regulation of the vertebrate oocyte-to-embryo transition
Fuentes R, Marlow F, Abrams E, Zhang H, Kobayashi M, Gupta T, Kapp L, DiNardo Z, Heller R, Cisternas R, García-Castro P, Segovia-Miranda F, Montecinos-Franjola F, Vought W, Vejnar C, Giraldez A, Mullins M. Maternal regulation of the vertebrate oocyte-to-embryo transition. PLOS Genetics 2024, 20: e1011343. PMID: 39052672, PMCID: PMC11302925, DOI: 10.1371/journal.pgen.1011343.Peer-Reviewed Original ResearchOocyte-to-embryo transitionMutant geneZebrafish forward genetic screenEgg activationForward genetic screensGenetic entry pointsMaternal-effect mutantsBasic body planCytoplasmic segregationBody planIntracellular traffickingGenetic screeningMicrotubule nucleationProtein cleavageMaternal genesVesicle formationGenesFunctional importanceHuman diseasesMolecular mechanismsMolecular insightsCortical granulesBlack caviarMaternal regulationMolecular nature
2019
Brd4 and P300 Confer Transcriptional Competency during Zygotic Genome Activation
Chan SH, Tang Y, Miao L, Darwich-Codore H, Vejnar CE, Beaudoin JD, Musaev D, Fernandez JP, Benitez MDJ, Bazzini AA, Moreno-Mateos MA, Giraldez AJ. Brd4 and P300 Confer Transcriptional Competency during Zygotic Genome Activation. Developmental Cell 2019, 49: 867-881.e8. PMID: 31211993, PMCID: PMC7201981, DOI: 10.1016/j.devcel.2019.05.037.Peer-Reviewed Original ResearchConceptsGenome activationTranscriptional competencyHistone acetylationP300-dependent histone acetylationZygotic genome activationFirst zygotic genesMaternal mRNA translationZygotic genesAnimal developmentZygotic developmentDevelopmental reprogrammingMRNA translationLive imagingCell cycleSilent genomeBRD4ZebrafishGenomeTranscriptionAcetylationActivationP300FertilizationReprogrammingGenes
2018
Analyses of mRNA structure dynamics identify embryonic gene regulatory programs
Beaudoin JD, Novoa EM, Vejnar CE, Yartseva V, Takacs CM, Kellis M, Giraldez AJ. Analyses of mRNA structure dynamics identify embryonic gene regulatory programs. Nature Structural & Molecular Biology 2018, 25: 677-686. PMID: 30061596, PMCID: PMC6690192, DOI: 10.1038/s41594-018-0091-z.Peer-Reviewed Original ResearchConceptsGene regulatory programsUntranslated regionRegulatory programsKey maternal factorsZygotic transitionZebrafish developmentGene functionRNA functionRegulatory elementsGene expressionRNA structureRNA foldingBiological transitionsStructure dynamicsCentral roleCrucial roleCellular systemsRemodelersTranscriptomeRibosomesVivoGenesFoldingCCNA1Role
2014
Identification of small ORFs in vertebrates using ribosome footprinting and evolutionary conservation
Bazzini AA, Johnstone TG, Christiano R, Mackowiak SD, Obermayer B, Fleming ES, Vejnar CE, Lee MT, Rajewsky N, Walther TC, Giraldez AJ. Identification of small ORFs in vertebrates using ribosome footprinting and evolutionary conservation. The EMBO Journal 2014, 33: 981-993. PMID: 24705786, PMCID: PMC4193932, DOI: 10.1002/embj.201488411.Peer-Reviewed Original Research
2013
Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition
Lee MT, Bonneau AR, Takacs CM, Bazzini AA, DiVito KR, Fleming ES, Giraldez AJ. Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition. Nature 2013, 503: 360-364. PMID: 24056933, PMCID: PMC3925760, DOI: 10.1038/nature12632.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCellular ReprogrammingEmbryonic DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalHomeodomain ProteinsMicroRNAsMothersNanog Homeobox ProteinOctamer Transcription Factor-3Pluripotent Stem CellsRibosomesSOXB1 Transcription FactorsTranscriptomeZebrafishZebrafish ProteinsZygoteConceptsZygotic genome activationZygotic transitionMiR-430Zygotic gene expressionZygotic gene activationZygotic developmental programMaternal messenger RNAsZygotic programGenome activationZygotic genesZygotic transcriptionRibosome profilingDevelopmental programGene activationTranscription factorsDevelopmental arrestGene expressionSoxB1POU5F1Maternal programMessenger RNANanogGastrulationZebrafishGenes
2006
Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs
Giraldez AJ, Mishima Y, Rihel J, Grocock RJ, Van Dongen S, Inoue K, Enright AJ, Schier AF. Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs. Science 2006, 312: 75-79. PMID: 16484454, DOI: 10.1126/science.1122689.Peer-Reviewed Original ResearchMeSH Keywords3' Untranslated RegionsAdenosineAnimalsEmbryo, NonmammalianEmbryonic DevelopmentFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGenes, ReporterGreen Fluorescent ProteinsMicroRNAsMutationOligonucleotide Array Sequence AnalysisPolymersProtein BiosynthesisRibonuclease IIIRNA StabilityRNA, MessengerTranscription, GeneticUp-RegulationZebrafishZygoteConceptsMessenger RNA moleculesTarget messenger RNA moleculesMiR-430Vivo target validationVertebrate genesZygotic transcriptionEarly embryogenesisRNA moleculesMicroarray approachVivo functionDeadenylationTarget validationEarly developmentMechanism of actionMost targetsEmbryogenesisMorphogenesisTranscriptionGenesMicroRNAsTarget