Coherent Raman tissue imaging in the brain.
Saar BG, Freudiger CW, Xu X, Huttner A, Kesari S, Young G, Xie XS. Coherent Raman tissue imaging in the brain. Cold Spring Harbor Protocols 2014, 2014: pdb.top081695. PMID: 24786507, DOI: 10.1101/pdb.top081695.Peer-Reviewed Original ResearchConceptsMultiphoton-excited fluorescenceCRS imagingCRS techniqueCoherent anti-Stokes RamanCoherent Raman scattering techniquesAnti-Stokes RamanLabel-free chemical imagingLabel-free imagingClass of moleculesDrug moleculesRaman scattering techniqueVibrational signaturesChemical imagingFluorescent moleculesScattering techniquesRaman scatteringHigh spatial resolutionMolecular speciesMultiphoton microscopySimilar imaging parametersEndogenous fluorophoresMoleculesSpatial resolutionFluorescent labelingInstrumentation requirements