1995
Modulation of calcium currents by a D1 dopaminergic protein kinase/phosphatase cascade in rat neostriatal neurons
Surmeier D, Bargas J, Hemmings H, Nairn A, Greengard P. Modulation of calcium currents by a D1 dopaminergic protein kinase/phosphatase cascade in rat neostriatal neurons. Neuron 1995, 14: 385-397. PMID: 7531987, DOI: 10.1016/0896-6273(95)90294-5.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthine2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl esterAnimalsBrainCalcium Channel BlockersCalcium ChannelsCells, CulturedColforsinCyclic AMPCyclic AMP-Dependent Protein KinasesElectrophysiologyKineticsMembrane PotentialsNeostriatumNeuronsNifedipinePhosphoprotein PhosphatasesProtein Phosphatase 1RatsRats, WistarReceptors, Dopamine D1TetraethylammoniumTetraethylammonium CompoundsTime FactorsConceptsProtein phosphatase 1Protein kinaseInhibition of PP1Cyclic AMP-dependent protein kinaseAMP-dependent protein kinaseInhibition of PKARat neostriatal neuronsPhosphatase cascadePP1 activityReceptor-mediated activationPhosphatase 1Neostriatal neuronsCalcium currentPKA enhancementDifferential regulationHigh voltage-activated calcium currentsVoltage-activated calcium currentsWhole-cell voltage-clamp techniqueD1 pathwayMedium spiny neuronsCyclic AMP analogueD1 dopamine receptorsL-type currentDiversity of effectsSubset of neurons
1994
Identification of the phosphorylation site for cAMP-dependent protein kinase on Na+,K(+)-ATPase and effects of site-directed mutagenesis.
Fisone G, Cheng S, Nairn A, Czernik A, Hemmings H, Höög J, Bertorello A, Kaiser R, Bergman T, Jörnvall H. Identification of the phosphorylation site for cAMP-dependent protein kinase on Na+,K(+)-ATPase and effects of site-directed mutagenesis. Journal Of Biological Chemistry 1994, 269: 9368-9373. PMID: 7510709, DOI: 10.1016/s0021-9258(17)37117-x.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthineAmino Acid SequenceAnimalsBase SequenceColforsinCyclic AMP-Dependent Protein KinasesDNA PrimersKineticsMolecular Sequence DataMutagenesis, Site-DirectedPeptide MappingPeptidesPhosphoserineRatsRecombinant ProteinsSodium-Potassium-Exchanging ATPaseStructure-Activity RelationshipConceptsCAMP-dependent protein kinasePhosphorylation sitesProtein kinaseSignal transduction pathwaysWild-type enzymeSite-directed mutagenesisATPase alpha subunitAlpha 1 isoformCatalytic subunitTransduction pathwaysDependent phosphorylationSeryl residuesCOS cellsAlpha subunitIntact cellsATPaseKinasePhosphorylationEnzymeSubunitsCellsExperimental approachMutagenesisCDNAIsoforms
1990
Nerve Growth Factor‐Induced Down‐Regulation of Calmodulin‐Dependent Protein Kinase III in PC12 Cells Involves Cyclic AMP‐Dependent Protein Kinase
Brady M, Nairn A, Wagner J, Palfrey H. Nerve Growth Factor‐Induced Down‐Regulation of Calmodulin‐Dependent Protein Kinase III in PC12 Cells Involves Cyclic AMP‐Dependent Protein Kinase. Journal Of Neurochemistry 1990, 54: 1034-1039. PMID: 1689374, DOI: 10.1111/j.1471-4159.1990.tb02354.x.Peer-Reviewed Original ResearchConceptsWild-type cellsCalmodulin-dependent protein kinase IIICAMP-dependent protein kinase activityProtein kinase IIIProtein kinase activityEpidermal growth factorKinase IIIKinase activityA126-1B2 cellsCyclic AMP-dependent protein kinaseAMP-dependent protein kinasePC12 cellsNerve growth factorMutant PC12 cell lineElongation factor 2Growth factorAbility of NGFPC12 cell lineEffects of NGFProtein kinaseNGF additionA126-1B2Down regulationCell linesFactor 2
1987
Nerve growth factor treatment or cAMP elevation reduces Ca2+/calmodulin-dependent protein kinase III activity in PC12 cells.
Nairn A, Nichols R, Brady M, Palfrey H. Nerve growth factor treatment or cAMP elevation reduces Ca2+/calmodulin-dependent protein kinase III activity in PC12 cells. Journal Of Biological Chemistry 1987, 262: 14265-14272. PMID: 2443502, DOI: 10.1016/s0021-9258(18)47933-1.Peer-Reviewed Original ResearchConceptsNerve growth factorEffect of forskolinIII activityPC12 cellsGrowth factorLong-term treatmentShort-term treatmentEffect of treatmentNerve growth factor treatmentGrowth factor treatmentAbility of forskolinAbility of NGFPhorbol esterEpidermal growth factorMaximal effectCytosolic extractsFactor treatmentForskolinCAMP elevationTreatmentCell linesProtein kinase CRemoval of forskolinImmunological techniquesDependent protein phosphorylation