Preparation of acute subventricular zone slices for calcium imaging.
Lacar B, Young SZ, Platel JC, Bordey A. Preparation of acute subventricular zone slices for calcium imaging. Journal Of Visualized Experiments 2012, e4071. PMID: 23023088, PMCID: PMC3490239, DOI: 10.3791/4071.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsMeSH KeywordsAniline CompoundsAnimalsAstrocytesCalciumFluorescent DyesLateral VentriclesMiceMicroscopy, ConfocalMicrotomyXanthenesConceptsCell typesSVZ cellsProgenitor cellsNeurogenic zonesSubventricular zoneFluo-4 AM dyeNeural progenitor cellsIntermediate progenitor cellsExtracellular signalsDiffusible signalsIntercellular signalingSVZ cell typesTime-lapse moviesCalcium indicator dyeCalcium activityIndividual cellsRostral-caudal axisNeuroblast migrationThree-dimensional arrangementAdult hippocampal subgranular zonePostnatal brainAdhesion moleculesHippocampal subgranular zoneMature astrocytesNeuroblasts