2000
Muscarinic Activation of BK Channels Induces Membrane Oscillations in Glioma Cells and Leads to Inhibition of Cell Migration
Bordey A, Sontheimer H, Trouslard J. Muscarinic Activation of BK Channels Induces Membrane Oscillations in Glioma Cells and Leads to Inhibition of Cell Migration. The Journal Of Membrane Biology 2000, 176: 31-40. PMID: 10882426, DOI: 10.1007/s00232001073.Peer-Reviewed Original ResearchConceptsPatch-clamp recordingsGlioma cellsAstrocytoma cellsCell migrationCultured human astrocytoma cellsIntracellular free calcium concentrationACh receptor agonistsFree calcium concentrationHuman astrocytoma cellsRelease of Ca2Glioma cell migrationMuscarinic activationMuscarinic antagonistCerebral tumorsMuscarinic receptorsReceptor agonistBath applicationActivate Ca2Intracellular injectionCerebrospinal fluidACh applicationAcetylcholineBiphasic increaseIntracellular storesExternal calcium
1998
Expression of voltage-activated chloride currents in acute slices of human gliomas
Ullrich N, Bordey A, Gillespie GY, Sontheimer H. Expression of voltage-activated chloride currents in acute slices of human gliomas. Neuroscience 1998, 83: 1161-1173. PMID: 9502255, DOI: 10.1016/s0306-4522(97)00456-9.Peer-Reviewed Original ResearchConceptsAcute slicesChloride currentsGlioma cellsGrade tumorsGlial cellsPatient biopsiesWhole-cell patch-clamp recordingsChloride channelsHost glial cellsImplanted glioma cellsPathological tumor gradeLow-grade tumorsHigh-grade tumorsPatch-clamp recordingsSpecific Cl- channelsNormal glial cellsAstrocyte-like cellsShort-term cultureTumor gradePathological gradeSCID miceVoltage-activated sodiumIntracranial injectionPotassium currentExperimental tumors
1994
Mobilization of intracellular calcium by substance p in a human astrocytoma cell line (U‐373 MG)
Bordey A, Feltz P, Trouslard J. Mobilization of intracellular calcium by substance p in a human astrocytoma cell line (U‐373 MG). Glia 1994, 11: 277-283. PMID: 7525479, DOI: 10.1002/glia.440110309.Peer-Reviewed Original ResearchConceptsSubstance PReceptor agonistConcentrations of SPEffects of SPIntracellular free calcium concentrationSelective receptor agonistsNK1 receptor agonistNM substance PIntracellular calcium poolsFura-2 acetoxymethylesterHuman astrocytoma cell lineStable basal levelExtracellular calcium ionsFree calcium concentrationAstrocytoma cell linesHuman astrocytoma cellsPertussis treatmentNK1 receptorsIntracellular calciumCalcium poolMaximal effectCytosolic calciumAstrocytoma cellsBasal levelsCalcium concentrationSR 140333, a Novel, Selective, and Potent Nonpeptide Antagonist of the NK1 Tachykinin Receptor: Characterization on the U373MG Cell Line
Oury‐Donat F, Lefevre IA, Thurneyssen O, Gauthier T, Bordey A, Feltz P, Emonds‐Alt X, Le Fur G, Soubrie P. SR 140333, a Novel, Selective, and Potent Nonpeptide Antagonist of the NK1 Tachykinin Receptor: Characterization on the U373MG Cell Line. Journal Of Neurochemistry 1994, 62: 1399-1407. PMID: 7510780, DOI: 10.1046/j.1471-4159.1994.62041399.x.Peer-Reviewed Original ResearchMeSH KeywordsAstrocytomaBinding, CompetitiveCalciumElectric ConductivityGenes, fosHumansInositol PhosphatesIodine RadioisotopesNeurokinin-1 Receptor AntagonistsPeptide FragmentsPiperidinesPotassiumPyrrolidonecarboxylic AcidQuinuclidinesReceptors, Neurokinin-1StereoisomerismSubstance PSuccinimidesTaurineTranscription, GeneticTumor Cells, CulturedConceptsSR 140333Substance PNK1 tachykinin receptor antagonistNK1 receptor activationHunter substance PNK1-selective agonistTachykinin receptor antagonistsInositol monophosphate formationNK1 tachykinin receptorsPotent nonpeptide antagonistHuman astrocytoma cell lineCell linesAstrocytoma cell linesPatch-clamp experimentsReceptor antagonistFormation of inositolTachykinin receptorsNK1 agonistU373MG cell linesCalcium mobilizationNonpeptide antagonistsRadioligand bindingReceptor activationOutward currentsMonophosphate formation