2022
Expression of the transcription factor PU.1 induces the generation of microglia-like cells in human cortical organoids
Cakir B, Tanaka Y, Kiral FR, Xiang Y, Dagliyan O, Wang J, Lee M, Greaney AM, Yang WS, duBoulay C, Kural MH, Patterson B, Zhong M, Kim J, Bai Y, Min W, Niklason LE, Patra P, Park IH. Expression of the transcription factor PU.1 induces the generation of microglia-like cells in human cortical organoids. Nature Communications 2022, 13: 430. PMID: 35058453, PMCID: PMC8776770, DOI: 10.1038/s41467-022-28043-y.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsHuman cortical organoidsTranscription factor PUSingle-cell RNA sequencingMicroglia-like cellsSingle-cell transcriptomicsEmbryonic stem cellsDisease stage IIIRole of microgliaAD-associated genesExpression of genesCortical organoidsNeurodegenerative disordersRNA sequencingMolecular damageIntact complementStem cellsDysfunction of microgliaFunctional microgliaReduced expressionGenesCell clustersExpressionChemokine systemHuman microglia
2020
Stem Cells, Cell Therapies, and Bioengineering in Lung Biology and Disease 2019
Wagner D, Ikonomou L, Gilpin S, Magin C, Cruz F, Greaney A, Magnusson M, Chen Y, Davis B, Vanuytsel K, Enes S, Krasnodembskaya A, Lehmann M, Westergren-Thorsson G, Stegmayr J, Alsafadi H, Hoffman E, Weiss D, Ryan A. Stem Cells, Cell Therapies, and Bioengineering in Lung Biology and Disease 2019. ERJ Open Research 2020, 6: 00123-2020. PMID: 33123557, PMCID: PMC7569162, DOI: 10.1183/23120541.00123-2020.Peer-Reviewed Original ResearchCell therapyProgenitor cellsLung stemLung biologyInduced pluripotent stem cell modelsInternational Society for CellTreatment of lung diseasesPluripotent stem cell modelsPulmonary Fibrosis FoundationCystic Fibrosis FoundationStem cell modelRegenerative medicine productsAlpha-1 FoundationUniversity of Vermont Larner College of MedicineGene therapyCystic fibrosisLung diseaseCollege of MedicineTherapyStem cellsAlpha 1LungBlood InstituteNational HeartExtracellular vesicles