2014
Tubular Vascular Endothelial Growth Factor-A, Erythropoietin, and Medullary Vessels: A Trio Linked by Hypoxia
Tufro A. Tubular Vascular Endothelial Growth Factor-A, Erythropoietin, and Medullary Vessels: A Trio Linked by Hypoxia. Journal Of The American Society Of Nephrology 2014, 26: 997-998. PMID: 25385850, PMCID: PMC4413774, DOI: 10.1681/asn.2014101004.Peer-Reviewed Original ResearchPodocyte-Specific VEGF-A Gain of Function Induces Nodular Glomerulosclerosis in eNOS Null Mice
Veron D, Aggarwal PK, Velazquez H, Kashgarian M, Moeckel G, Tufro A. Podocyte-Specific VEGF-A Gain of Function Induces Nodular Glomerulosclerosis in eNOS Null Mice. Journal Of The American Society Of Nephrology 2014, 25: 1814-1824. PMID: 24578128, PMCID: PMC4116059, DOI: 10.1681/asn.2013070752.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Culture TechniquesCollagen Type IVDiabetic NephropathiesGlomerular Basement MembraneLamininMiceMice, KnockoutNitric Oxide Synthase Type IIIPodocytesProteinuriaRenal InsufficiencyVascular Endothelial Growth Factor AConceptsNodular glomerulosclerosisGain of functionEndothelial nitric oxide synthase knockout miceNitric oxide synthase knockout miceGlomerular basement membrane thickeningENOS-null miceSynthase knockout miceBasement membrane thickeningWild-type miceCollagen IVArteriolar hyalinosisGlomerular nodulesGlomerular VEGFKimmelstiel-WilsonPronounced albuminuriaCreatinine clearanceRenal failureDiabetic nephropathyENOS deficiencyMassive proteinuriaDiabetic milieuMembrane thickeningPodocyte effacementDeposition of lamininKnockout mice
2012
Acute Podocyte Vascular Endothelial Growth Factor (VEGF-A) Knockdown Disrupts alphaVbeta3 Integrin Signaling in the Glomerulus
Veron D, Villegas G, Aggarwal PK, Bertuccio C, Jimenez J, Velazquez H, Reidy K, Abrahamson DR, Moeckel G, Kashgarian M, Tufro A. Acute Podocyte Vascular Endothelial Growth Factor (VEGF-A) Knockdown Disrupts alphaVbeta3 Integrin Signaling in the Glomerulus. PLOS ONE 2012, 7: e40589. PMID: 22808199, PMCID: PMC3396653, DOI: 10.1371/journal.pone.0040589.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood PressureCells, CulturedDown-RegulationDoxycyclineEndotheliumFibronectinsGene Knockdown TechniquesIntegrin alphaVbeta3MiceModels, AnimalNeuropilin-1PhenotypePodocytesProtein BindingProteinuriaRenal InsufficiencyRNA, Small InterferingSignal TransductionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsAcute renal failureVEGF receptor 2Renal failureEndothelial cell swellingPodocyte VEGFUrine VEGFGlomerular filtration barrierLocal injuryPodocyte effacementGlomerular ultrastructureAdult miceDoxycycline exposureReceptor 2Knockdown micePodocyte cell lineControl valuesGlomeruliNeuropilin-1MiceVEGFProtein levelsCell swellingVEGF knockdownProteinuriaFiltration barrier
2011
Vascular Endothelial Growth Factor Receptor 2 Direct Interaction with Nephrin Links VEGF-A Signals to Actin in Kidney Podocytes*
Bertuccio C, Veron D, Aggarwal PK, Holzman L, Tufro A. Vascular Endothelial Growth Factor Receptor 2 Direct Interaction with Nephrin Links VEGF-A Signals to Actin in Kidney Podocytes*. Journal Of Biological Chemistry 2011, 286: 39933-39944. PMID: 21937443, PMCID: PMC3220571, DOI: 10.1074/jbc.m111.241620.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdaptor Proteins, Signal TransducingAnimalsChlorocebus aethiopsCOS CellsMass SpectrometryMembrane ProteinsMiceMice, TransgenicMultiprotein ComplexesOncogene ProteinsPhosphorylationPodocytesProtein Structure, TertiarySignal TransductionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsVEGF receptor 2Cytoplasmic domainTransmembrane protein nephrinBlot overlay experimentsSpecialized cell junctionsFoot process structurePodocyte actin cytoskeletonExtracellular cuesActin cytoskeletonGrowth factorMultiprotein interactionsTyrosine phosphorylationOverlay experimentsPodocyte cytoskeletonPodocyte foot processesCell junctionsPodocyte shapeCultured cellsKidney podocytesProtein nephrinSlit diaphragmDirect interactionCytoskeletonEpithelial cellsCultured podocytesPodocyte vascular endothelial growth factor (Vegf164) overexpression causes severe nodular glomerulosclerosis in a mouse model of type 1 diabetes
Veron D, Bertuccio CA, Marlier A, Reidy K, Garcia AM, Jimenez J, Velazquez H, Kashgarian M, Moeckel GW, Tufro A. Podocyte vascular endothelial growth factor (Vegf164) overexpression causes severe nodular glomerulosclerosis in a mouse model of type 1 diabetes. Diabetologia 2011, 54: 1227-1241. PMID: 21318407, PMCID: PMC3397150, DOI: 10.1007/s00125-010-2034-z.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternChromatography, High Pressure LiquidCreatinineDiabetes Mellitus, Type 1Diabetic NephropathiesDisease Models, AnimalEnzyme-Linked Immunosorbent AssayImmunohistochemistryMiceMice, TransgenicMicroscopy, Electron, TransmissionPodocytesPolymerase Chain ReactionSemaphorin-3ATandem Mass SpectrometryVascular Endothelial Growth Factor AConceptsDiabetic nephropathyNodular glomerulosclerosisDiabetic glomerulopathyMouse modelMassive proteinuriaExcessive vascular endothelial growth factorTransgenic miceStreptozotocin-induced mouse modelVascular endothelial growth factor overexpressionGlomerular basement membrane thickeningAdvanced diabetic glomerulopathyControl diabetic miceOnset of diabetesBasement membrane thickeningVascular endothelial growth factorType 1 diabetesGrowth factor overexpressionAdult transgenic miceEndothelial growth factorVEGF receptor 2Kimmelstiel-WilsonSystemic VEGFDiabetic micePathogenic roleRenal morphology
2010
Induction of Podocyte VEGF164 Overexpression at Different Stages of Development Causes Congenital Nephrosis or Steroid-Resistant Nephrotic Syndrome
Veron D, Reidy K, Marlier A, Bertuccio C, Villegas G, Jimenez J, Kashgarian M, Tufro A. Induction of Podocyte VEGF164 Overexpression at Different Stages of Development Causes Congenital Nephrosis or Steroid-Resistant Nephrotic Syndrome. American Journal Of Pathology 2010, 177: 2225-2233. PMID: 20829436, PMCID: PMC2966782, DOI: 10.2353/ajpath.2010.091146.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsHumansKidneyMiceMice, TransgenicNephrosis, LipoidNephrotic SyndromePodocytesProteinuriaVascular Endothelial Growth Factor AConceptsNephrotic syndromePodocyte effacementTransgenic miceSteroid-resistant nephrotic syndromeEndothelial cellsSingle transgenic miceMultiple renal diseasesSwollen endothelial cellsCongenital nephrotic syndromeVascular endothelial growthInducible transgenic miceNormal endothelial cellsGlomerular filtration barrierRenal diseasePathogenic rolePodocyte lossMice expressMassive albuminuriaEndothelial growthCongenital nephrosisMinimal changesFoot processesMiceGlomerulomegalyAlbuminuriaOverexpression of VEGF-A in podocytes of adult mice causes glomerular disease
Veron D, Reidy KJ, Bertuccio C, Teichman J, Villegas G, Jimenez J, Shen W, Kopp JB, Thomas DB, Tufro A. Overexpression of VEGF-A in podocytes of adult mice causes glomerular disease. Kidney International 2010, 77: 989-999. PMID: 20375978, DOI: 10.1038/ki.2010.64.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAutocrine CommunicationDiabetic NephropathiesGenotypeGlomerular Basement MembraneKidney DiseasesMatrix Metalloproteinase 9Membrane ProteinsMiceMice, TransgenicParacrine CommunicationPhenotypePhosphorylationPodocytesProtein BindingProteinuriaSignal TransductionUp-RegulationVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ConceptsGlomerular diseaseAdult miceGlomerular basement membrane thickeningMurine diabetic nephropathyBasement membrane thickeningGlomerular endothelial cellsAdult transgenic miceOverexpression of VEGFExcessive VEGFDiabetic nephropathyGlomerular filtration barrierMesangial expansionPathogenic roleMetalloproteinase-9Functional abnormalitiesMembrane thickeningPodocyte effacementNephrin expressionReceptor 2Transgenic miceWhole kidneyGlomerular phenotypeEndothelial cellsParacrine VEGFVEGF
2007
Semaphorin3a inhibits ureteric bud branching morphogenesis
Tufro A, Teichman J, Woda C, Villegas G. Semaphorin3a inhibits ureteric bud branching morphogenesis. Cells And Development 2007, 125: 558-568. PMID: 18249526, PMCID: PMC3992265, DOI: 10.1016/j.mod.2007.12.003.Peer-Reviewed Original ResearchConceptsUreteric budUreteric bud branchingAkt survival pathwayUreteric bud treeUreteric bud cellsAntisense morpholinoClass 3 semaphorinsVascular patterningMetanephric organ cultureFactor signalingMouse embryosNegative regulatorKidney developmentSurvival pathwaysGuidance proteinsNeurotrophic Factor SignalingSema3A effectBudsRenal epitheliumMorphogenesisBud cellsMutant miceRecombinant Sema3AGrowth factorSema3ASemaphorin3a disrupts podocyte foot processes causing acute proteinuria
Tapia R, Guan F, Gershin I, Teichman J, Villegas G, Tufro A. Semaphorin3a disrupts podocyte foot processes causing acute proteinuria. Kidney International 2007, 73: 733-740. PMID: 18075495, DOI: 10.1038/sj.ki.5002726.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCytoskeletal ProteinsDown-RegulationGlomerular Basement MembraneGlomerular Filtration RateIntracellular Signaling Peptides and ProteinsMaleMembrane ProteinsMiceMice, Inbred StrainsPermeabilityPodocytesProteinuriaRecombinant ProteinsSemaphorin-3AVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-1Vascular Endothelial Growth Factor Receptor-2ConceptsPodocyte foot process effacementNephrotic range proteinuriaEndothelial cell damageVascular endothelial growth factorSlit diaphragm protein podocinFoot process effacementEndothelial growth factorReceptor 2 signalingVascular endothelial growth factor receptor 2 signalingAcute proteinuriaRange proteinuriaReceptor expressionVascular endothelial growth factor 165Process effacementBarrier homeostasisAdult mouse kidneyUltrastructural abnormalitiesSemaphorin3AEndothelial cellsCell damageGrowth factorMouse kidneyGuidance proteinsProteinuriaDownregulationCrosstalk between VEGF-A/VEGFR2 and GDNF/RET signaling pathways
Tufro A, Teichman J, Banu N, Villegas G. Crosstalk between VEGF-A/VEGFR2 and GDNF/RET signaling pathways. Biochemical And Biophysical Research Communications 2007, 358: 410-416. PMID: 17490619, DOI: 10.1016/j.bbrc.2007.04.146.Peer-Reviewed Original ResearchConceptsVascular endothelial growth factorCell proliferationEndothelial growth factorNeurotrophic factorGDNF/RETEmbryonic kidney explantsUreteric bud branchingUreteric bud cellsC-RetGrowth factorRT-PCRVEGFNovel cooperative interactionVEGFR2Branching morphogenesisRETVEGFR2 autophosphorylationGDNFKidney developmentBud cellsUreteric budAdditive effectMajor regulatorKidney explantsPhosphorylation
2006
Semaphorin 3C regulates endothelial cell function by increasing integrin activity
Banu N, Teichman J, Dunlap‐Brown M, Villegas G, Tufro A, Banu N, Teichman J, Dunlap‐Brown M, Villegas G, Tufro A. Semaphorin 3C regulates endothelial cell function by increasing integrin activity. The FASEB Journal 2006, 20: 2150-2152. PMID: 16940438, DOI: 10.1096/fj.05-5698fje.Peer-Reviewed Original ResearchConceptsSema 3CEndothelial cell functionDirectional migrationStarvation-induced apoptosisCell functionCapillary-like network formationCardiovascular patterningClass 3 semaphorinsIntegrin phosphorylationSerine phosphorylationCollagen I gelsVascular morphogenesisEndothelial cellsMouse glomerular endothelial cellsIntegrin activityGuidance proteinsEndothelial cell proliferationIntegrin inhibitionCaspase-3Cell proliferationSitu markerTube formationSemaphorin 3CPhosphorylationGrowth factorAutocrine VEGF-A system in podocytes regulates podocin and its interaction with CD2AP
Guan F, Villegas G, Teichman J, Mundel P, Tufro A. Autocrine VEGF-A system in podocytes regulates podocin and its interaction with CD2AP. American Journal Of Physiology. Renal Physiology 2006, 291: f422-f428. PMID: 16597608, DOI: 10.1152/ajprenal.00448.2005.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsC-Reactive ProteinCell DifferentiationCell LineCell SurvivalCytoskeletal ProteinsGene Expression RegulationIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceNerve Tissue ProteinsNeuropilin-2PodocytesProteinsProto-Oncogene Proteins c-aktReceptors, Vascular Endothelial Growth FactorRNA, MessengerSignal TransductionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-1Vascular Endothelial Growth Factor Receptor-2ConceptsWestern analysisAutocrine VEGFPodocyte VEGFGrowth factor signalingVEGFR2 mRNA levelsMRNA levelsEndothelial cell differentiationVascular endothelial growth factor signalingCompletion of developmentVascular patterningKidney morphogenesisFactor signalingPodocyte differentiationUndifferentiated podocytesLigand availabilityCell differentiationVEGFR2 phosphorylationDifferentiated conditionsImmortalized mouse podocytesProtein levelsProtein expressionMouse podocytesVEGFR2 mRNADifferentiated podocytesCD2AP
2005
Autocrine and paracrine functions of vascular endothelial growth factor (VEGF) in renal tubular epithelial cells
Villegas G, Lange-Sperandio B, Tufro A. Autocrine and paracrine functions of vascular endothelial growth factor (VEGF) in renal tubular epithelial cells. Kidney International 2005, 67: 449-457. PMID: 15673292, DOI: 10.1111/j.1523-1755.2005.67101.x.Peer-Reviewed Original Research