2024
Disturbed flow in the juxta-anastomotic area of an arteriovenous fistula correlates with endothelial loss, acute thrombus formation, and neointimal hyperplasia
Bai H, Varsanik M, Thaxton C, Ohashi Y, Gonzalez L, Zhang W, Aoyagi Y, Kano M, Yatsula B, Li Z, Pocivavsek L, Dardik A. Disturbed flow in the juxta-anastomotic area of an arteriovenous fistula correlates with endothelial loss, acute thrombus formation, and neointimal hyperplasia. AJP Heart And Circulatory Physiology 2024, 326: h1446-h1461. PMID: 38578237, PMCID: PMC11380968, DOI: 10.1152/ajpheart.00054.2024.Peer-Reviewed Original ResearchConceptsEndothelial cell lossOutflow veinChronic kidney diseaseJuxta-anastomotic areaMouse AVF modelNeointimal hyperplasiaThrombus formationAcute thrombus formationCell lossHuman AVF maturationGene OntologyAVF maturationAVF patencyEarly thrombus formationArteriovenous fistulaC57BL/6 miceClinical failureWistar ratsEndothelial lossKidney diseaseImmune responseEndothelial cellsHyperplasiaMiceAVF
2020
Artery to vein configuration of arteriovenous fistula improves hemodynamics to increase maturation and patency
Bai H, Sadaghianloo N, Gorecka J, Liu S, Ono S, Ramachandra AB, Bonnet S, Mazure NM, Declemy S, Humphrey JD, Dardik A. Artery to vein configuration of arteriovenous fistula improves hemodynamics to increase maturation and patency. Science Translational Medicine 2020, 12 PMID: 32817365, PMCID: PMC7705473, DOI: 10.1126/scitranslmed.aax7613.Peer-Reviewed Original ResearchConceptsArteriovenous fistulaHemodialysis accessNeointimal hyperplasiaEndothelial nitric oxide synthaseDurable surgical optionUremic male ratsRadial-cephalic arteriovenous fistulaKruppel-like factor 2Nitric oxide synthasePeak systolic velocityLong-term patencyReintervention rateSecondary patencyImproved hemodynamicsFemale patientsReimplantation techniqueSurgical optionsSystolic velocityMale ratsOxide synthaseFistula modelPatencyHemodynamicsDifferent hemodynamicsFemale animals
1999
Neutralizing TGF-β1 antibody infusion in neonatal rat delays in vivo glomerular capillary formation 1 1 See Editorial by Abrahamson, p. 1597
Liu A, Dardik A, Ballermann B. Neutralizing TGF-β1 antibody infusion in neonatal rat delays in vivo glomerular capillary formation 1 1 See Editorial by Abrahamson, p. 1597. Kidney International 1999, 56: 1334-1348. PMID: 10504486, DOI: 10.1046/j.1523-1755.1999.00661.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBinding, CompetitiveCapillariesCell DifferentiationEndothelial Growth FactorsEndothelium, VascularFemaleImmunoglobulin GInjections, Intra-ArterialIodine RadioisotopesKidney GlomerulusLymphokinesMicroinjectionsMicroscopy, ElectronNephronsNeutralization TestsPregnancyRatsRats, Sprague-DawleyTime FactorsTransforming Growth Factor betaVascular Endothelial Growth Factor AVascular Endothelial Growth FactorsVon Willebrand FactorChronic in vitro shear stress stimulates endothelial cell retention on prosthetic vascular grafts and reduces subsequent in vivo neointimal thickness
Dardik A, Liu A, Ballermann B. Chronic in vitro shear stress stimulates endothelial cell retention on prosthetic vascular grafts and reduces subsequent in vivo neointimal thickness. Journal Of Vascular Surgery 1999, 29: 157-167. PMID: 9882800, DOI: 10.1016/s0741-5214(99)70357-5.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsBeta-GalactosidaseBlood Vessel ProsthesisCell AdhesionCells, CulturedEndothelium, VascularFemaleGenetic VectorsHemorheologyHemostasisIn Vitro TechniquesMicroscopy, Electron, ScanningPolyurethanesRatsRats, Inbred F344Stress, MechanicalThrombosisTransfectionTunica IntimaConceptsProsthetic vascular graftsNeointimal thicknessDyne/cm2 shear stressEndothelial cellsGraft thrombosisEndothelial cell monolayersEndothelial cell retentionHemostasis timeVascular graftsCell monolayersAlpha-smooth muscle actinFischer 344 rat aortaGraft patency rateReplication-deficient adenoviral vectorLuminal surfaceAortic interposition graftsImmediate graft thrombosisChronic shear stressCultured endothelial cellsGraft failureInterposition graftMacrophage infiltrationPatency ratesCell-seeded graftsRat aorta