2024
Disturbed flow in the juxta-anastomotic area of an arteriovenous fistula correlates with endothelial loss, acute thrombus formation, and neointimal hyperplasia
Bai H, Varsanik M, Thaxton C, Ohashi Y, Gonzalez L, Zhang W, Aoyagi Y, Kano M, Yatsula B, Li Z, Pocivavsek L, Dardik A. Disturbed flow in the juxta-anastomotic area of an arteriovenous fistula correlates with endothelial loss, acute thrombus formation, and neointimal hyperplasia. AJP Heart And Circulatory Physiology 2024, 326: h1446-h1461. PMID: 38578237, PMCID: PMC11380968, DOI: 10.1152/ajpheart.00054.2024.Peer-Reviewed Original ResearchConceptsEndothelial cell lossOutflow veinChronic kidney diseaseJuxta-anastomotic areaMouse AVF modelNeointimal hyperplasiaThrombus formationAcute thrombus formationCell lossHuman AVF maturationGene OntologyAVF maturationAVF patencyEarly thrombus formationArteriovenous fistulaC57BL/6 miceClinical failureWistar ratsEndothelial lossKidney diseaseImmune responseEndothelial cellsHyperplasiaMiceAVF
2022
Endothelial Cell TGF-β (Transforming Growth Factor-Beta) Signaling Regulates Venous Adaptive Remodeling to Improve Arteriovenous Fistula Patency
Taniguchi R, Ohashi Y, Lee JS, Hu H, Gonzalez L, Zhang W, Langford J, Matsubara Y, Yatsula B, Tellides G, Fahmy TM, Hoshina K, Dardik A. Endothelial Cell TGF-β (Transforming Growth Factor-Beta) Signaling Regulates Venous Adaptive Remodeling to Improve Arteriovenous Fistula Patency. Arteriosclerosis Thrombosis And Vascular Biology 2022, 42: 868-883. PMID: 35510552, PMCID: PMC9233042, DOI: 10.1161/atvbaha.122.317676.Peer-Reviewed Original ResearchConceptsArteriovenous fistulaSMC proliferationAVF patencyCollagen densityMouse aortocaval fistula modelTGF-β receptor IArteriovenous fistula patencyAortocaval fistula modelInhibition of TGFPredetermined time pointsTGF-β inhibitionTGF-β signalingTGF-β receptorDisruption of TGFFistula patencyAVF failureWall thicknessVascular accessVenous remodelingSuccessful hemodialysisDoppler ultrasoundFistula modelReceptor IPatencyTGF
2020
TGFβ (Transforming Growth Factor-Beta)–Activated Kinase 1 Regulates Arteriovenous Fistula Maturation
Hu H, Lee SR, Bai H, Guo J, Hashimoto T, Isaji T, Guo X, Wang T, Wolf K, Liu S, Ono S, Yatsula B, Dardik A. TGFβ (Transforming Growth Factor-Beta)–Activated Kinase 1 Regulates Arteriovenous Fistula Maturation. Arteriosclerosis Thrombosis And Vascular Biology 2020, 40: e203-e213. PMID: 32460580, PMCID: PMC7316601, DOI: 10.1161/atvbaha.119.313848.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAortaArteriovenous Shunt, SurgicalCells, CulturedCollagen Type IEndothelial CellsFibronectinsHumansJNK Mitogen-Activated Protein KinasesMaleMAP Kinase Kinase KinasesMechanotransduction, CellularMice, Inbred C57BLP38 Mitogen-Activated Protein KinasesPhosphorylationStress, MechanicalVascular PatencyVascular RemodelingVena Cava, InferiorConceptsArteriovenous fistulaAVF maturationNoncanonical TGFβSuccessful AVF maturationArteriovenous fistula maturationNovel therapeutic approachesTAK1 functionsFistula maturationHemodialysis accessDeposition of ECMLuminal diameterMouse endothelial cellsMaturation failureTherapeutic approachesLumen dilationControl veinsWall thickeningAVF modelOptimal conduitArterial magnitudesEndothelial cellsTAK1 phosphorylationCollagen 1Growth factorLaminar shear stress
2010
Expression and localization of vascular endothelial growth factor in normal abdominal aorta and abdominal aortic aneurysm.
Nishibe T, Dardik A, Kondo Y, Kudo F, Muto A, Nishi M, Nishibe M, Shigematsu H. Expression and localization of vascular endothelial growth factor in normal abdominal aorta and abdominal aortic aneurysm. International Angiology 2010, 29: 260-5. PMID: 20502414.Peer-Reviewed Original ResearchConceptsVascular endothelial growth factorAbdominal aortic aneurysmSmooth muscle cellsAortic aneurysmEndothelial growth factorEndothelial cellsAbdominal aortaPositive vascular endothelial growth factorNormal abdominal aortaAbdominal aortic aneurysm surgeryLocalization of VEGFExpression of VEGFGrowth factorNormal aortic samplesAbdominal aortic aneurysm samplesAortic aneurysm surgeryAneurysm samplesHuman abdominal aortic aneurysmImmunofluorescent double stainingAbdominal aorta samplesAneurysm surgeryRegulators of angiogenesisNormal human aortaAneurysm formationAutopsy specimens
2003
Shear Stress and Cyclic Strain May Suppress Apoptosis in Endothelial Cells by Different Pathways
Haga M, Chen A, Gortler D, Dardik A, Sumpio B. Shear Stress and Cyclic Strain May Suppress Apoptosis in Endothelial Cells by Different Pathways. Endothelium 2003, 10: 149-157. PMID: 13129818, DOI: 10.1080/10623320390233463.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBcl-Associated Death ProteinCarrier ProteinsCattleCells, CulturedCulture Media, Serum-FreeEndothelial CellsEnzyme InhibitorsHemodynamicsPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationProtein Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktSignal TransductionStress, MechanicalTranscription, GeneticConceptsPhosphorylation of AktEndothelial cellsPI3K inhibitionInhibition of apoptosisK inhibitionHemodynamic forcesAortic endothelial cellsBovine aortic endothelial cellsEffect of SSArterial levelsIntimal hyperplasiaDownstream target BadNormal endotheliumMaximal stimulationAkt phosphorylationLaminar shear stressApoptosisDownstream phosphorylationAktInhibitionCell survivalEC growthSerum withdrawalAlternate pathwayPhosphorylation of Bad