2019
Control of Bacterial Virulence through the Peptide Signature of the Habitat
Krypotou E, Scortti M, Grundström C, Oelker M, Luisi B, Sauer-Eriksson A, Vázquez-Boland J. Control of Bacterial Virulence through the Peptide Signature of the Habitat. Cell Reports 2019, 26: 1815-1827.e5. PMID: 30759392, PMCID: PMC6389498, DOI: 10.1016/j.celrep.2019.01.073.Peer-Reviewed Original ResearchConceptsBacterial virulenceControl of bacterial virulenceFacultative intracellular bacterium Listeria monocytogenesOpp transport systemIntracellular bacterium Listeria monocytogenesBacterium Listeria monocytogenesGSH-binding siteVirulence programCo-crystallization studiesNutritional peptidesPrfAGenetic screeningTranscriptional activityOrganic N sourcesVirulenceNon-cysteine-containing peptidesSteric blockadeHost entryRegulation mechanismBinding sitesPeptide signaturesActive peptidesPeptideN sourcesOligopeptides
2015
Origin, diversification and substrate specificity in the family of NCS1/FUR transporters
Krypotou E, Evangelidis T, Bobonis J, Pittis A, Gabaldón T, Scazzocchio C, Mikros E, Diallinas G. Origin, diversification and substrate specificity in the family of NCS1/FUR transporters. Molecular Microbiology 2015, 96: 927-950. PMID: 25712422, DOI: 10.1111/mmi.12982.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAspergillus nidulansBinding SitesFungal ProteinsGene DuplicationGene Transfer, HorizontalMembrane Transport ProteinsMolecular Docking SimulationMolecular Dynamics SimulationMutationPhylogenyProtein ConformationProtein Structure, TertiaryPseudogenesSequence Homology, Amino AcidSubstrate SpecificitySymportersConceptsSubstrate specificitySubstrate binding siteFunctional diversificationModel fungus Aspergillus nidulansSystematic mutational analysisBinding sitesStructure-function analysisUptake of purinesNCS1 proteinsPlant homologuesFur proteinAspergillus nidulansGene duplicationHorizontal transferSubstrate dockingMutation analysisSub-familyHomology modelingMolecular mechanismsNCS1ProteinResiduesFurDiversificationNidulans