2019
Control of Bacterial Virulence through the Peptide Signature of the Habitat
Krypotou E, Scortti M, Grundström C, Oelker M, Luisi B, Sauer-Eriksson A, Vázquez-Boland J. Control of Bacterial Virulence through the Peptide Signature of the Habitat. Cell Reports 2019, 26: 1815-1827.e5. PMID: 30759392, PMCID: PMC6389498, DOI: 10.1016/j.celrep.2019.01.073.Peer-Reviewed Original ResearchConceptsBacterial virulenceControl of bacterial virulenceFacultative intracellular bacterium Listeria monocytogenesOpp transport systemIntracellular bacterium Listeria monocytogenesBacterium Listeria monocytogenesGSH-binding siteVirulence programCo-crystallization studiesNutritional peptidesPrfAGenetic screeningTranscriptional activityOrganic N sourcesVirulenceNon-cysteine-containing peptidesSteric blockadeHost entryRegulation mechanismBinding sitesPeptide signaturesActive peptidesPeptideN sourcesOligopeptides
2015
Functional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine–uric acid transporter and an intrinsically misfolded polypeptide
Krypotou E, Scazzocchio C, Diallinas G. Functional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine–uric acid transporter and an intrinsically misfolded polypeptide. Fungal Genetics And Biology 2015, 75: 56-63. PMID: 25639910, DOI: 10.1016/j.fgb.2015.01.009.Peer-Reviewed Original ResearchConceptsNucleobase-ascorbate transporterUptake of purinesAcid transportAspergillus nidulansMisfolded polypeptidesER-retainedGFP tagMisfolded proteinsHeterologous expressionEvolutionary implicationsInactive proteinPutative transportersAspergillus brasiliensisFunctional characterizationTurned-overPlasma membranePeptide sequencesIn silicoProteinSubfamilyAspergillusTransport functionLow affinityHigher affinityVacuoles