Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens
Klock G, Wisnewski AV, el-Bassiouni EA, Ramadan MI, Gessner P, Zimmermann U, Kresina TF. Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens. Monoclonal Antibodies In Immunodiagnosis And Immunotherapy 1992, 11: 469-481. PMID: 1398684, DOI: 10.1089/hyb.1992.11.469.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyMonoclonal antibodiesIgG antibodiesParasite antigensHypo-osmolar electrofusionS. mansoniSchistosoma mansoni wormsCell linesWestern blot analysisEgg antigenParasitic antigensAntigen reactivityAntigenAntibodiesSplenocytesBlot analysisHybridomasCandidate moleculesMansoniHybridoma generationMitogenCell fusionSomatic cell fusionGreater numberELISA