Direct targeting of amplified gene loci for proapoptotic anticancer therapy
Kaushik Tiwari M, Colon-Rios DA, Tumu HCR, Liu Y, Quijano E, Krysztofiak A, Chan C, Song E, Braddock DT, Suh HW, Saltzman WM, Rogers FA. Direct targeting of amplified gene loci for proapoptotic anticancer therapy. Nature Biotechnology 2021, 40: 325-334. PMID: 34711990, PMCID: PMC8930417, DOI: 10.1038/s41587-021-01057-5.Peer-Reviewed Original ResearchConceptsDNA double-strand breaksTriplex-forming oligonucleotidesDNA damage responseDouble-strand breaksDrug resistanceGene amplificationP53-independent apoptosisHER2-positive breastOvarian cancer modelHuman tumor xenograftsInduction of apoptosisGenomic lociNumber of drugsCellular functionsDamage responseGene locusProtein productsHER2-positive cancer cellsDriver genesClinical efficacyCombat drug resistanceDNA damageHER2 amplificationTherapeutic strategiesTumor xenograftsHigh-throughput Evaluation of Protein Migration and Localization after Laser Micro-Irradiation
Oeck S, Malewicz NM, Krysztofiak A, Turchick A, Jendrossek V, Glazer PM. High-throughput Evaluation of Protein Migration and Localization after Laser Micro-Irradiation. Scientific Reports 2019, 9: 3148. PMID: 30816253, PMCID: PMC6395640, DOI: 10.1038/s41598-019-39760-8.Peer-Reviewed Original ResearchConceptsDNA damage sitesDNA repair proteinsProtein of interestHistone dynamicsHistone phosphorylationProtein fociHigh-throughput analysisProtein modificationRepair proteinsDNA damageDamage sitesSingle nucleusMultiple fluorescence channelsHigh-throughput evaluationRepair kineticsProtein migrationProteinAnalysis of accumulationFluorescence channelsPhosphorylationDNACellsAccumulationLocalizationMigration