2021
Cryo-EM structure of an open conformation of a gap junction hemichannel in lipid bilayer nanodiscs
Khan A, Jagielnicki M, Bennett B, Purdy M, Yeager M. Cryo-EM structure of an open conformation of a gap junction hemichannel in lipid bilayer nanodiscs. Structure 2021, 29: 1040-1047.e3. PMID: 34129834, PMCID: PMC9616683, DOI: 10.1016/j.str.2021.05.010.Peer-Reviewed Original ResearchMeSH KeywordsConnexin 26HumansLipid BilayersMolecular Dynamics SimulationProtein Conformation, alpha-HelicalConceptsLipid bilayer nanodiscsGap junction channelsHexameric hemichannelsExtracellular loopBilayer nanodiscsTransmembrane α-helicesCryo-EM structureGap junction hemichannelsAdjacent cell membranesIntercellular conduitsCell communicationCx hemichannelsPlasma membraneOpen conformationSecond extracellular loopJunction channelsΑ-helixConformational flexibilityCell membraneHemichannelsCx isoformsExtracellular spaceNanodiscsStructural foundationMembrane
2011
Molecular Origin of Electron Paramagnetic Resonance Line Shapes on β-Barrel Membrane Proteins: The Local Solvation Environment Modulates Spin-Label Configuration
Freed D, Khan A, Horanyi P, Cafiso D. Molecular Origin of Electron Paramagnetic Resonance Line Shapes on β-Barrel Membrane Proteins: The Local Solvation Environment Modulates Spin-Label Configuration. Biochemistry 2011, 50: 8792-8803. PMID: 21894979, PMCID: PMC3199607, DOI: 10.1021/bi200971x.Peer-Reviewed Original ResearchMeSH KeywordsBacterial Outer Membrane ProteinsBinding SitesCrystallography, X-RayElectron Spin Resonance SpectroscopyEscherichia coliEscherichia coli ProteinsLipid BilayersMembrane ProteinsMembrane Transport ProteinsModels, MolecularMutagenesisProtein ConformationProtein Structure, SecondaryProtein Structure, TertiarySolventsSpin LabelsConceptsLine shapeLong-range distance restraintsEPR spectraResonance line shapeΒ-barrel membrane proteinsDifferent rotameric statesFast rotationRotameric statesElectron paramagnetic resonance spectroscopyEPR line shapeParamagnetic resonance spectroscopyMulticomponent spectraElectron paramagnetic resonance line shapesSpectraWeak interactionsProtein-lipid interfaceAtomic resolution structuresDistance restraintsBilayer thicknessLipid environmentLipid bilayersStructure refinementMembrane-facing surfaceProtein structure refinementResonance spectroscopy