2015
Periosteal PTHrP Regulates Cortical Bone Remodeling During Fracture Healing
Wang M, Nasiri AR, Broadus AE, Tommasini SM. Periosteal PTHrP Regulates Cortical Bone Remodeling During Fracture Healing. Bone 2015, 81: 104-111. PMID: 26164475, PMCID: PMC4641003, DOI: 10.1016/j.bone.2015.07.008.Peer-Reviewed Original ResearchConceptsFracture healingPTHrP expressionCKO miceCortical bone surfaceFracture repairTibial fracture surgeryRole of PTHrPHormone-related proteinTibial fracture modelCartilaginous callus formationConditional knockout miceBone surfaceCortical bone remodelingType I receptorCD1 controlsFracture surgeryCD1 miceKnockout miceInitial genetic evidencePTHrPOsteoblastic activityBone remodelingMiceBone mineralizationI receptorResponsive population dynamics and wide seeding into the duodenal lamina propria of transglutaminase-2-specific plasma cells in celiac disease
Di Niro R, Snir O, Kaukinen K, Yaari G, Lundin K, Gupta N, Kleinstein S, Cols M, Cerutti A, Mäki M, Shlomchik M, Sollid L. Responsive population dynamics and wide seeding into the duodenal lamina propria of transglutaminase-2-specific plasma cells in celiac disease. Mucosal Immunology 2015, 9: 254-264. PMID: 26153762, PMCID: PMC4703456, DOI: 10.1038/mi.2015.57.Peer-Reviewed Original ResearchMeSH KeywordsAutoantibodiesBiopsyCeliac DiseaseCell CountDiet, Gluten-FreeDuodenumGene Expression RegulationGlutensGTP-Binding ProteinsHumansImmunoglobulin Heavy ChainsIntestinal MucosaLaser Capture MicrodissectionPlasma CellsProtein Glutamine gamma Glutamyltransferase 2Sequence Analysis, DNATransglutaminasesConceptsTG2-specific plasma cellsPlasma cellsCeliac diseaseLamina propriaTransglutaminase 2Antibody-mediated diseasesGluten-free dietSerum antibody levelsSerum antibody titersB cell responsesAntigen-specific antibodiesDuodenal lamina propriaGluten exposureUntreated patientsAntibody levelsAntibody titersCeliac lesionAntigen stainingSubepithelial layerAntibody productionIndividual biopsiesRepertoire analysisDiseaseGut tissueAntibodiesAltered Expression of CDC42 Signaling Pathway Components in Cortical Layer 3 Pyramidal Cells in Schizophrenia
Datta D, Arion D, Corradi J, Lewis D. Altered Expression of CDC42 Signaling Pathway Components in Cortical Layer 3 Pyramidal Cells in Schizophrenia. Biological Psychiatry 2015, 78: 775-785. PMID: 25981171, PMCID: PMC4600637, DOI: 10.1016/j.biopsych.2015.03.030.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntipsychotic AgentsBenzodiazepinescdc42 GTP-Binding ProteinCytoskeletal ProteinsFemaleGene Expression RegulationGTP-Binding Protein RegulatorsHaloperidolHumansLaser Capture MicrodissectionLim KinasesMacaca fascicularisMaleMiddle AgedOlanzapinep21-Activated KinasesPrefrontal Cortexrho GTP-Binding Proteinsrho Guanine Nucleotide Dissociation Inhibitor alphaRNA-Binding ProteinsSchizophreniaSignal TransductionConceptsSerine/threonine protein kinaseThreonine protein kinaseActin cytoskeletonProtein kinaseCdc42 effector proteinsCell type-specific fashionMessenger RNA levelsCell division cycle 42Altered expressionType-specific fashionDLPFC gray matterRNA levelsEffector proteinsActin dynamicsPathway componentsSame transcriptKey organizerMessenger RNALaser microdissectionSynergistic alterationsCdc42CytoskeletonQuantitative polymerase chain reactionSpine formationKinaseDistinctive transcriptome alterations of prefrontal pyramidal neurons in schizophrenia and schizoaffective disorder
Arion D, Corradi J, Tang S, Datta D, Boothe F, He A, Cacace A, Zaczek R, Albright C, Tseng G, Lewis D. Distinctive transcriptome alterations of prefrontal pyramidal neurons in schizophrenia and schizoaffective disorder. Molecular Psychiatry 2015, 20: 1397-1405. PMID: 25560755, PMCID: PMC4492919, DOI: 10.1038/mp.2014.171.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnalysis of VarianceAnimalsAntipsychotic AgentsFemaleGene Expression ProfilingGene Expression RegulationHumansLaser Capture MicrodissectionMacaca fascicularisMaleMiddle AgedMitochondrial ProteinsOligonucleotide Array Sequence AnalysisPrefrontal CortexPsychotic DisordersPyramidal CellsRNA, MessengerSchizophreniaSignal TransductionTranscriptomeUbiquitinIdentification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection
Esaki H, Ewald DA, Ungar B, Rozenblit M, Zheng X, Xu H, Estrada YD, Peng X, Mitsui H, Litman T, Suárez-Fariñas M, Krueger JG, Guttman-Yassky E. Identification of novel immune and barrier genes in atopic dermatitis by means of laser capture microdissection. Journal Of Allergy And Clinical Immunology 2015, 135: 153-163. PMID: 25567045, PMCID: PMC4452382, DOI: 10.1016/j.jaci.2014.10.037.Peer-Reviewed Original ResearchMeSH KeywordsAdultDermatitis, AtopicFemaleGene Expression ProfilingHumansLaser Capture MicrodissectionMaleMiddle AgedOligonucleotide Array Sequence AnalysisReal-Time Polymerase Chain ReactionSkinConceptsNonlesional AD skinLaser capture microdissectionAD transcriptomeNormal skinAD skinNonlesional skinNovel ImmuneCapture microdissectionAtopic dermatitis lesionsBarrier genesPossible cellular sourcesAtopic dermatitisHealthy volunteersEpidermal alterationsBarrier phenotypeCellular sourceImmune moleculesCellular subsetsDermatitis lesionsImmuneDermal compartmentSkinGenomic profilesPatientsMolecular signatures
2014
Developmental Expression Patterns of GABAA Receptor Subunits in Layer 3 and 5 Pyramidal Cells of Monkey Prefrontal Cortex
Datta D, Arion D, Lewis D. Developmental Expression Patterns of GABAA Receptor Subunits in Layer 3 and 5 Pyramidal Cells of Monkey Prefrontal Cortex. Cerebral Cortex 2014, 25: 2295-2305. PMID: 24610118, PMCID: PMC4494034, DOI: 10.1093/cercor/bhu040.Peer-Reviewed Original ResearchConceptsGABAA receptor subunitsPyramidal cellsMonkey prefrontal cortexReceptor subunitsSubunit expressionLayer 3 pyramidal cellsGABAA receptor subunit expressionPrefrontal cortexGABAA receptor subunit mRNAsLayer 5 pyramidal cellsReceptor subunit changesPrefrontal pyramidal neuronsReceptor subunit expressionPyramidal neuron activityGlutamate receptor subunitsReceptor subunit mRNAsPyramidal cell firingPostnatal developmental changesPyramidal neuronsGABAA receptorsInhibitory inputsNeuron activityFunctional maturationPostnatal developmentCell firingProduction of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
Bandyopadhyay U, Fenton WA, Horwich AL, Nagy M. Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection. Journal Of Visualized Experiments 2014, 51168. PMID: 24457537, PMCID: PMC4089401, DOI: 10.3791/51168.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAzure StainsGene Expression ProfilingLaser Capture MicrodissectionMiceNeuronsRNASpinal CordConceptsLaser capture microdissectionMotor neuronsSpinal cordMotor neuron cell bodiesCapture microdissectionMouse spinal cordNeuron cell bodiesTotal cell populationCell typesPharmacologic treatmentStained neuronsCell bodiesNeurodegenerative diseasesNeuronsExogenous RNasesQRT-PCRSame cell typeCell populationsCordRNA damageDiseaseTotal RNASuch preparationsMicrodissectionTranscriptional differences
2012
Matrix Metalloproteinase-19 Is a Key Regulator of Lung Fibrosis in Mice and Humans
Yu G, Kovkarova-Naumovski E, Jara P, Parwani A, Kass D, Ruiz V, Lopez-Otín C, Rosas IO, Gibson KF, Cabrera S, Ramírez R, Yousem SA, Richards TJ, Chensny LJ, Selman M, Kaminski N, Pardo A. Matrix Metalloproteinase-19 Is a Key Regulator of Lung Fibrosis in Mice and Humans. American Journal Of Respiratory And Critical Care Medicine 2012, 186: 752-762. PMID: 22859522, PMCID: PMC5450991, DOI: 10.1164/rccm.201202-0302oc.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBleomycinCells, CulturedCyclooxygenase 2Epithelial CellsGene Expression Regulation, EnzymologicHumansIdiopathic Pulmonary FibrosisLaser Capture MicrodissectionMatrix Metalloproteinases, SecretedMiceMice, KnockoutOligonucleotide Array Sequence AnalysisPulmonary AlveoliUp-RegulationConceptsIdiopathic pulmonary fibrosisHyperplastic epithelial cellsAlveolar epithelial cellsEpithelial cellsMMP-19IPF lungsWT miceLung fibrosisFibrotic responseHyperplastic alveolar epithelial cellsNovel mediatorLaser capture microscopeLung fibrotic responseDevelopment of fibrosisWild-type miceEpithelial phenotypic changesMatrix metalloproteinase-19Microarray analysisA549 epithelial cellsLung injuryBronchoalveolar lavagePulmonary fibrosisLung tissueSame lungFibrosisClinicopathological Characteristics and Molecular Analyses of Multifocal Intraductal Papillary Mucinous Neoplasms of the Pancreas
Matthaei H, Norris AL, Tsiatis AC, Olino K, Hong SM, dal Molin M, Goggins MG, Canto M, Horton KM, Jackson KD, Capelli P, Zamboni G, Bortesi L, Furukawa T, Egawa S, Ishida M, Ottomo S, Unno M, Motoi F, Wolfgang CL, Edil BH, Cameron JL, Eshleman JR, Schulick RD, Maitra A, Hruban RH. Clinicopathological Characteristics and Molecular Analyses of Multifocal Intraductal Papillary Mucinous Neoplasms of the Pancreas. Annals Of Surgery 2012, 255: 326-333. PMID: 22167000, PMCID: PMC3534752, DOI: 10.1097/sla.0b013e3182378a18.Peer-Reviewed Original ResearchMeSH KeywordsAdenocarcinoma, MucinousAgedAged, 80 and overCarcinoma, Pancreatic DuctalCarcinoma, PapillaryClone CellsFemaleHumansLaser Capture MicrodissectionLoss of HeterozygosityMaleMiddle AgedMutationNeoplasms, Multiple PrimaryNeoplasms, Second PrimaryPancreatic NeoplasmsProto-Oncogene ProteinsProto-Oncogene Proteins p21(ras)ras ProteinsRetrospective StudiesSequence Analysis, DNAConceptsMultifocal intraductal papillary mucinous neoplasmsIntraductal papillary mucinous neoplasmPapillary mucinous neoplasmMucinous neoplasmsClinicopathologic featuresPancreatic cancerClonal relationshipBranch duct lesionsCystic precursor lesionsFamilial pancreatic cancerKRAS gene mutationsIntermediate dysplasiaIndependent genetic alterationsDuct lesionsClinicopathological characteristicsInvasive cancerPrecursor lesionsPartial pancreatectomyPatientsNeoplasmsIntermediate gradeGene mutationsCancerGenetic alterationsMolecular analysis
2011
Direct and Indirect Contribution of Human Embryonic Stem Cell–Derived Hepatocyte-Like Cells to Liver Repair in Mice
Woo D, Kim S, Lim H, Heo J, Park H, Kang G, Kim S, You H, Hoeppner D, Kim Y, Kwon H, Choi T, Lee J, Hong S, Song K, Ahn E, Chenoweth J, Tesar P, McKay R, Kim J. Direct and Indirect Contribution of Human Embryonic Stem Cell–Derived Hepatocyte-Like Cells to Liver Repair in Mice. Gastroenterology 2011, 142: 602-611. PMID: 22138358, DOI: 10.1053/j.gastro.2011.11.030.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersCarbon TetrachlorideCell DifferentiationCell ProliferationCell SeparationCells, CulturedChemical and Drug Induced Liver InjuryCoculture TechniquesDisease Models, AnimalEmbryonic Stem CellsHepatocytesHumansImmunohistochemistryInduced Pluripotent Stem CellsLaser Capture MicrodissectionLithium ChlorideLiverLiver RegenerationMass SpectrometryMiceMice, Inbred BALB CMice, NudeMicroscopy, ElectronNeovascularization, PhysiologicPolymerase Chain ReactionProteomicsTime FactorsWound HealingConceptsHepatocyte-like cellsLiver repairLiver tissueCell replacementEndogenous liver regenerationAcute liver injuryDirect cell replacementHost liver tissueStem cellsLiver of miceIndocyanine green stainingHuman embryonic stem cell-derived cellsEmbryonic stem cell-derived cellsCell-derived signalsHost tissue repairStem cell-derived cellsStem cell-derived hepatocyte-like cellsCell-derived hepatocyte-like cellsLiver injuryCell-derived cellsPolymerase chain reactionCell graftsIntraperitoneal injectionHepatic featuresTrophic factors
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