2016
Novel anatomic adaptation of cortical bone to meet increased mineral demands of reproduction
Macica CM, King HE, Wang M, McEachon CL, Skinner CW, Tommasini SM. Novel anatomic adaptation of cortical bone to meet increased mineral demands of reproduction. Bone 2016, 85: 59-69. PMID: 26825813, PMCID: PMC7429445, DOI: 10.1016/j.bone.2015.12.056.Peer-Reviewed Original ResearchConceptsHyp miceMineral homeostasisElevated serum PTHMaternal bone massMineralized trabecular boneIntestinal calcium absorptionCortical boneWild-type miceSerum PTHCalcitriol productionFemale patientsMaternal skeletonPostnatal nutritionCalcium absorptionBone resorptionFracture riskMaternal adaptationBone massBone fragilityMurine modelIntracortical porosityUnaffected miceMouse modelMMP-13Fetal development
2014
Activation of β-catenin signalling leads to temporomandibular joint defects
Wang M, Li S, Xie W, Shen J, Im H, Holz J, Wang M, Diekwisch T, Chen D. Activation of β-catenin signalling leads to temporomandibular joint defects. ECells & Materials 2014, 28: 223-235. PMID: 25340802, PMCID: PMC4288590, DOI: 10.22203/ecm.v028a15.Peer-Reviewed Original ResearchMeSH KeywordsADAM ProteinsADAMTS5 ProteinAnimalsApoptosisbeta CateninCartilageCell ProliferationMatrix Metalloproteinase 13MiceSignal TransductionTemporomandibular JointConceptsDouble mutant miceTMJ disordersΒ-cateninMutant miceADAMTS5-/- miceRole of MMP13OA-like phenotypeTemporomandibular joint disordersMG reporter micePotential therapeutic targetDevelopment of TMJTMJ cartilage degenerationHip osteoarthritisTMJ samplesJoint disordersTherapeutic targetReporter miceTransgenic miceCartilage degenerationMiceTMJ cartilageADAMTS5 geneTMJADAMTS5MMP13
2013
Deletion of the Transforming Growth Factor β Receptor Type II Gene in Articular Chondrocytes Leads to a Progressive Osteoarthritis‐like Phenotype in Mice
Shen J, Li J, Wang B, Jin H, Wang M, Zhang Y, Yang Y, Im H, O'Keefe R, Chen D. Deletion of the Transforming Growth Factor β Receptor Type II Gene in Articular Chondrocytes Leads to a Progressive Osteoarthritis‐like Phenotype in Mice. Arthritis & Rheumatism 2013, 65: 3107-3119. PMID: 23982761, PMCID: PMC3928444, DOI: 10.1002/art.38122.Peer-Reviewed Original ResearchMeSH KeywordsADAM ProteinsADAMTS5 ProteinAnimalsCartilage, ArticularChondrocytesCore Binding Factor Alpha 2 SubunitDisease ProgressionMatrix Metalloproteinase 13MiceMice, KnockoutOsteoarthritisPhenotypeProtein Serine-Threonine KinasesReceptor, Transforming Growth Factor-beta Type IIReceptors, Transforming Growth Factor betaSignal TransductionUp-RegulationConceptsCritical downstream target genesDownstream target genesTarget genesGrowth factor β signalingRegulation of Runx2Mouse genetic approachesLoss of TGFβType II geneGene expression analysisInhibition of TGFβDevelopment of osteoarthritisRat chondrosarcoma cellsGenetic approachesExpression analysisConditional knockout miceTGFβ signalingOsteoarthritis-like phenotypeGenesΒ signalingADAMTS5 geneReceptor type IIChondrosarcoma cellsTGFβCartilage homeostasisOA-like phenotypeIntracellular signaling mechanisms associated with CD47 modified surfaces
Finley M, Clark K, Alferiev I, Levy R, Stachelek S. Intracellular signaling mechanisms associated with CD47 modified surfaces. Biomaterials 2013, 34: 8640-8649. PMID: 23948164, PMCID: PMC3804223, DOI: 10.1016/j.biomaterials.2013.07.088.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultBloodCD47 AntigenChemokinesCytokinesDown-RegulationFemaleHumansJanus Kinase 2MaleMatrix Metalloproteinase 1Matrix Metalloproteinase 13Matrix Metalloproteinase 7Middle AgedPolyvinyl ChlorideSignal TransductionSTAT Transcription FactorsTranscription, GeneticTranscriptional ActivationUp-RegulationYoung AdultConceptsTranscription factor bindingDifferential molecular responsesJAK/STATChromatin associationCell signaling levelsSynthetic polymeric surfacesTranscriptional changesFactor bindingDownregulation of cytokinesJAK-STATRelated genesUpregulation of MMPsInflammatory cell responseExposure of bloodInflammatory cell interactionsMolecular responseTranscriptionQPCR arrayCell interactionsInflammatory responseHuman whole bloodCell responsesCD47BloodWhole bloodMMP13 is a critical target gene during the progression of osteoarthritis
Wang M, Sampson ER, Jin H, Li J, Ke QH, Im HJ, Chen D. MMP13 is a critical target gene during the progression of osteoarthritis. Arthritis Research & Therapy 2013, 15: r5. PMID: 23298463, PMCID: PMC3672752, DOI: 10.1186/ar4133.Peer-Reviewed Original ResearchConceptsMeniscal-ligamentous injuryOA progressionControl miceMMP13 activityType II collagenTUNEL stainingCre-negative control miceChondrocyte apoptosisEffective disease-modifying treatmentTerminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stainingTransferase dUTP nick end labeling stainingDUTP nick end labeling stainingRole of MMP13Nick end labeling stainingArticular cartilage lossCartilage gradingDisease-modifying treatmentsProgression of osteoarthritisWild-type miceDegenerative joint diseaseDevelopment of OAResults of immunohistochemistryEnd labeling stainingCre-negative miceArticular cartilage degeneration
2012
Conditional activation of β‐catenin signaling in mice leads to severe defects in intervertebral disc tissue
Wang M, Tang D, Shu B, Wang B, Jin H, Hao S, Dresser KA, Shen J, Im H, Sampson ER, Rubery PT, Zuscik MJ, Schwarz EM, O'Keefe RJ, Wang Y, Chen D. Conditional activation of β‐catenin signaling in mice leads to severe defects in intervertebral disc tissue. Arthritis & Rheumatism 2012, 64: 2611-2623. PMID: 22422036, PMCID: PMC3632450, DOI: 10.1002/art.34469.Peer-Reviewed Original ResearchConceptsDisc degenerationΒ-cateninDisc tissueExtensive osteophyte formationLow back painDisc tissue samplesIntervertebral disc degenerationPolymerase chain reaction assaysTissue functionBack painReal-time polymerase chain reaction assaysRunt-related transcription factorΒ-catenin protein levelsChain reaction assaysIntervertebral disc tissueOsteophyte formationΒ-catenin levelsImmunohistochemical analysisNormal subjectsMatrix metalloproteinase-13 inhibitorsTransgenic miceHistologic analysisΒ-catenin proteinADAMTS5 expressionGenetic ablation
2011
TGF‐β signaling plays an essential role in the growth and maintenance of intervertebral disc tissue
Jin H, Shen J, Wang B, Wang M, Shu B, Chen D. TGF‐β signaling plays an essential role in the growth and maintenance of intervertebral disc tissue. FEBS Letters 2011, 585: 1209-1215. PMID: 21420963, PMCID: PMC3090135, DOI: 10.1016/j.febslet.2011.03.034.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Density Conservation AgentsCells, CulturedChondrocytesCollagen Type IIFemaleGrowth PlateHistocytochemistryIntervertebral DiscMaleMatrix Metalloproteinase 13MiceMice, KnockoutMice, TransgenicProtein Serine-Threonine KinasesReceptor, Transforming Growth Factor-beta Type IIReceptors, Transforming Growth Factor betaReverse Transcriptase Polymerase Chain ReactionSignal TransductionTamoxifenTime FactorsConceptsKnockout miceTgfbr2 conditional knockout miceMMP13 geneDisc tissueConditional knockout miceGrowth plate chondrocytesIntervertebral disc tissueAnnulus fibrosus cellsTGFBR2 geneTransgenic miceGrowth plate cartilagePostnatal stagesMiceNormal growth plate cartilagePlate cartilageDisc phenotypesDisc cellsNull backgroundPresent studyCartilage growthTissueCartilageCritical roleCellsEmbryonic stages
2005
A Putative Role for Psoriasin in Breast Tumor Progression
Krop I, März A, Carlsson H, Li X, Bloushtain-Qimron N, Hu M, Gelman R, Sabel MS, Schnitt S, Ramaswamy S, Kleer CG, Enerbäck C, Polyak K. A Putative Role for Psoriasin in Breast Tumor Progression. Cancer Research 2005, 65: 11326-11334. PMID: 16357139, DOI: 10.1158/0008-5472.can-05-1523.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisBiomarkers, TumorBreast NeoplasmsCalcium-Binding ProteinsCarcinoma, Intraductal, NoninfiltratingCollagenasesDisease ProgressionDown-RegulationFemaleHumansMatrix Metalloproteinase 13MiceMice, NudeNeovascularization, PathologicReceptors, EstrogenRNA, MessengerRNA, Small InterferingS100 Calcium Binding Protein A7S100 ProteinsTumor Cells, CulturedVascular Endothelial Growth Factor AConceptsReactive oxygen speciesInvasive breast carcinomaBreast tumor progressionVascular endothelial growth factorTumor progressionMitochondrial reactive oxygen speciesPsoriasin expressionStable short hairpin RNAShort hairpin RNAAnti-invasive functionPutative functionsHuman IBC cell linesEstrogen receptor-negative tumorsHuman invasive breast carcinomasHairpin RNAHigh psoriasin expressionIBC cell linesWorse clinical outcomesCell migrationPoor prognostic factorReceptor-negative tumorsPutative roleInhibits tumor growthMatrix metalloproteinase-13Cell proliferation
1998
Matrix metalloproteinase inhibition limits arterial enlargement in a rodent arteriovenous fistula model
Abbruzzese T, Guzman R, Martin R, Yee C, Zarins C, Dalman R. Matrix metalloproteinase inhibition limits arterial enlargement in a rodent arteriovenous fistula model. Surgery 1998, 124: 328-335. PMID: 9706156, DOI: 10.1016/s0039-6060(98)70138-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, AbdominalArteriovenous FistulaBlood PressureCarotid Artery DiseasesDisease Models, AnimalGelatinasesHeart RateIliac ArteryMaleMatrix Metalloproteinase 1Matrix Metalloproteinase 12Matrix Metalloproteinase 13Matrix Metalloproteinase 2Matrix Metalloproteinase 7Matrix Metalloproteinase 9Matrix Metalloproteinase InhibitorsMetalloendopeptidasesPyransRatsRats, Sprague-DawleyRegional Blood FlowConceptsMean arterial pressureArterial enlargementArterial pressureIliac diameterArteriovenous fistula modelFistula modelMale Sprague-Dawley ratsRat AVF modelPostoperative day 0Common iliac arterySprague-Dawley ratsBlood flow velocityMatrix metalloproteinase inhibitionStudent's t-testMatrix metalloproteinase inhibitorsIliac flowAVF groupAVF patencyArtery velocityInfrarenal aortaSham groupIliac arteryVehicle groupHeart rateTreatment groups
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