2021
Expression of human CD47 in pig glomeruli prevents proteinuria and prolongs graft survival following pig‐to‐baboon xenotransplantation
Takeuchi K, Ariyoshi Y, Shimizu A, Okumura Y, Cara‐Fuentes G, Garcia G, Pomposelli T, Watanabe H, Boyd L, Ekanayake‐Alper D, Amarnath D, Sykes M, Sachs D, Johnson R, Yamada K. Expression of human CD47 in pig glomeruli prevents proteinuria and prolongs graft survival following pig‐to‐baboon xenotransplantation. Xenotransplantation 2021, 28: e12708. PMID: 34418164, PMCID: PMC8957703, DOI: 10.1111/xen.12708.Peer-Reviewed Original ResearchConceptsDevelopment of proteinuriaKidney graftsRenal tubular cellsGlomerular cellsTubular cellsNephrotic syndromeHuman CD47Transgene expressionPig-to-baboon xenotransplantationBone marrow transplantationDestructive inflammatory responseUpregulation of CD80Pig-to-baboonSMPDL-3bPigsMarrow transplantationGalT-KOGraft survivalProlonged survivalAntibody administrationThymic graftsHCD47Kidney xenotransplantationVascularized thymic graftsGroup B
2020
Antibody reactivity with new antigens revealed in multi‐transgenic triple knockout pigs may cause early loss of pig kidneys in baboons
Ariyoshi Y, Takeuchi K, Pomposelli T, Ekanayake‐Alper D, Shimizu A, Boyd L, Estime E, Ohta M, Asfour A, Arn J, Ayares D, Lorber M, Sykes M, Sachs D, Yamada K. Antibody reactivity with new antigens revealed in multi‐transgenic triple knockout pigs may cause early loss of pig kidneys in baboons. Xenotransplantation 2020, 28: e12642. PMID: 32909301, PMCID: PMC8957702, DOI: 10.1111/xen.12642.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsCytidine monophospho-N-acetylneuraminic acid hydroxylaseEndothelial protein C receptorTransgenic pigsMulti-transgenesPigsPre-screening assayPig kidneyGene editing technologyPig-to-baboonRenal functionKO pigsEditing technologyVascularized thymic graftsBaboon seraKnockout pigsStable renal functionGalT-KOHuman thrombomodulinMaintained renal functionBlood mononuclear cellsAcute xenograft rejectionProtein C receptorGenetic manipulationNatural antibodies
2019
Intra‐bone bone marrow transplantation from hCD47 transgenic pigs to baboons prolongs chimerism to >60 days and promotes increased porcine lung transplant survival
Watanabe H, Ariyoshi Y, Pomposelli T, Takeuchi K, Ekanayake‐Alper D, Boyd L, Arn S, Sahara H, Shimizu A, Ayares D, Lorber M, Sykes M, Sachs D, Yamada K. Intra‐bone bone marrow transplantation from hCD47 transgenic pigs to baboons prolongs chimerism to >60 days and promotes increased porcine lung transplant survival. Xenotransplantation 2019, 27: e12552. PMID: 31544995, PMCID: PMC7007336, DOI: 10.1111/xen.12552.Peer-Reviewed Original ResearchConceptsLung xenograft survivalAnti-pig antibody levelsPig-to-baboonBone marrow transplantationNegative pigsLung xenograftsMarrow transplantationXenograft survivalPigsPig-to-baboon modelHCD47B cell toleranceLung transplant survivalCellular unresponsivenessAntibody responseLungs 1Antibody levelsGraft survivalProlonged survivalAnimalsT cellsHuman CD47MacrochimerismExtended survivalDays
2018
Durable Macrochimerism (>8 weeks) and Pig-Specific T and B Cell Unresponsiveness Following hCD47+ Transgenic GalTKO Pig Intra-Bone Bone Marrow Transplantation in Baboons
Watanabe H, Nomura S, Ariyoshi Y, Pomposelli T, Boyd L, Tasaki M, Ekanayake-Alper D, Glor H, Arn S, Hawley R, Sykes M, Sachs D, Yamada K. Durable Macrochimerism (>8 weeks) and Pig-Specific T and B Cell Unresponsiveness Following hCD47+ Transgenic GalTKO Pig Intra-Bone Bone Marrow Transplantation in Baboons. Transplantation 2018, 102: s391. DOI: 10.1097/01.tp.0000543158.20137.b0.Peer-Reviewed Original ResearchPorcine BM cellsBM cellsBone marrowNAb levelsT cell responses in vitroAntibody responsePorcine BMPig-to-non-human primate modelsPig-to-baboon modelMaintained blood levelsT cell responsesAnti-CD40L antibodyBone marrow transplantationB cell unresponsivenessB cell toleranceWhole-body irradiationResponses in vitroTransgenic pigsPig-to-baboonPig bone marrowConditioning regimenXenogeneic chimerismBody irradiationMarrow transplantationPersistent chimerism
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