2025
P-396 Improving post-ovarian transplant primordial follicle survival by neovascularizing approaches
Celik S, Erden M, Rho N, Molla W, Oktay K. P-396 Improving post-ovarian transplant primordial follicle survival by neovascularizing approaches. Human Reproduction 2025, 40: deaf097.702. DOI: 10.1093/humrep/deaf097.702.Peer-Reviewed Original ResearchPrimordial follicle lossPrimordial follicle densityFollicle lossSphingosine-1-PhosphateFlexHD PliableFTY-720Follicle activationOvarian graftsPrimordial folliclesLoss of primordial folliclesSevere-combined-immunodeficient miceFollicle densitySevere-combined-immunodeficiencyOvarian tissue transplantationPrimordial follicle activationPrimordial follicle survivalOvarian cortical piecesAcellular dermal matrixParaffin-embedded tissuesFollicle survivalCortical piecesChorion allograftTransplant outcomesCadaveric organ donorsWider implications
2024
Histologic Analysis of ‘Distraction Vaginogenesis’ in a Rat Model
Meyer H, Trosclair L, Clayton S, O’Quin C, Crochet C, Colvin J, Welch V, Alhaque A, Solitro G, Shah-Bruce M, Alexander J, Sorrells D. Histologic Analysis of ‘Distraction Vaginogenesis’ in a Rat Model. Pathophysiology 2024, 31: 298-308. PMID: 38921727, PMCID: PMC11206798, DOI: 10.3390/pathophysiology31020022.Peer-Reviewed Original ResearchVaginal agenesisStained with hematoxylin and eosin (H&ERat vaginal tissueSprague Dawley ratsLocal immune reactionHematoxylin and eosin (H&EParaffin-embedded tissuesMasson's trichrome stainingAnti-desmin antibodiesMullerian agenesisResin capEosin (H&EVaginal tissueSurgical vaginoplastyVaginal canalDesmin immunostainingCanal lengthSubmucosal eosinophiliaMechanical dilatationNeutrophilic inflammationPost-surgicalDawley ratsChronic inflammationRat modelTrichrome staining
2021
Detection of cytogenomic abnormalities by OncoScan microarray assay for products of conception from formalin-fixed paraffin-embedded and fresh fetal tissues
Wen J, Grommisch B, DiAdamo A, Chai H, Ng SME, Hui P, Bale A, Mak W, Wang G, Li P. Detection of cytogenomic abnormalities by OncoScan microarray assay for products of conception from formalin-fixed paraffin-embedded and fresh fetal tissues. Molecular Cytogenetics 2021, 14: 21. PMID: 33810806, PMCID: PMC8019165, DOI: 10.1186/s13039-021-00542-5.Peer-Reviewed Original ResearchPathogenic copy number variantsAbnormality detection rateCytogenomic abnormalitiesCulture failureNormal karyotypeHigh diagnostic yieldProducts of conceptionCell contaminationMaternal cell contaminationMolecular inversion probesFresh fetal tissuesParaffin-embedded tissuesFalse-negative resultsFetal resultsRetrospective studyPregnancy lossDiagnostic yieldClinical utilityMaternal cellsFetal tissuesAbnormalitiesNegative resultsChromosomal imbalancesChromosome abnormalitiesUnbalanced structural chromosome abnormalities
2020
Hot Spot TERT Promoter Mutations Are Rare in Sporadic Pancreatic Neuroendocrine Neoplasms and Associated with Telomere Length and Epigenetic Expression Patterns
Posch A, Hofer-Zeni S, Klieser E, Primavesi F, Naderlinger E, Brandstetter A, Filipits M, Urbas R, Swiercynski S, Jäger T, Winkelmann P, Kiesslich T, Lu L, Neureiter D, Stättner S, Holzmann K. Hot Spot TERT Promoter Mutations Are Rare in Sporadic Pancreatic Neuroendocrine Neoplasms and Associated with Telomere Length and Epigenetic Expression Patterns. Cancers 2020, 12: 1625. PMID: 32575418, PMCID: PMC7352723, DOI: 10.3390/cancers12061625.Peer-Reviewed Original ResearchTelomerase reverse transcriptase (TERT) promoter mutationsPancreatic neuroendocrine neoplasmsSporadic pancreatic neuroendocrine neoplasmsT allele frequencyC250TNeuroendocrine neoplasmsPNEN casesReverse transcriptase (TERT) promoter mutationsDisease-free survivalTrue clinical valueLarge patient cohortPromoter mutationsTERT promoter mutationsParaffin-embedded tissuesPNEN patientsTPM statusClinicopathological informationPatient cohortClinical valueHigh incidenceCancer subtypesAllele frequenciesRelative TLTelomere maintenance mechanismCancer cells
2019
The impact of RNA extraction method on accurate RNA sequencing from formalin-fixed paraffin-embedded tissues
Marczyk M, Fu C, Lau R, Du L, Trevarton AJ, Sinn BV, Gould RE, Pusztai L, Hatzis C, Symmans WF. The impact of RNA extraction method on accurate RNA sequencing from formalin-fixed paraffin-embedded tissues. BMC Cancer 2019, 19: 1189. PMID: 31805884, PMCID: PMC6896723, DOI: 10.1186/s12885-019-6363-0.Peer-Reviewed Original ResearchConceptsBreast cancerFresh frozenParaffin-embedded tumor samplesFF samplesFFPE samplesConcordance correlation coefficientMixed-effects model analysisBreast cancer signaturesQiagen RNeasy kitWhole transcriptome RNA sequencingParaffin-embedded tissuesTranscriptome RNA sequencingLinear mixed-effects model analysisGene expression signaturesDifferent kitsClinical trialsGene signatureTumor samplesGene expressionTranslational researchCancerTissue samplesExpression signaturesArchival formalinSimilar concordanceClinical Insignficance of Monoclonal T-Cell Populations and Duodenal Intraepithelial T-Cell Phenotypes in Celiac and Nonceliac Patients
Celli R, Hui P, Triscott H, Bogardus S, Gibson J, Hwang M, Robert ME. Clinical Insignficance of Monoclonal T-Cell Populations and Duodenal Intraepithelial T-Cell Phenotypes in Celiac and Nonceliac Patients. The American Journal Of Surgical Pathology 2019, 43: 151-160. PMID: 30334829, DOI: 10.1097/pas.0000000000001172.Peer-Reviewed Original ResearchConceptsRefractory celiac diseaseT cell populationsMonoclonal T-cell populationRCD IRCD IICeliac diseasePersistent symptomsCD patientsIntraepithelial lymphocytesType II refractory celiac diseaseCD8-positive intraepithelial lymphocytesClonal T-cell populationsT-cell receptor gene rearrangementsAbnormal intraepithelial lymphocytesT-cell phenotypeReceptor gene rearrangementsParaffin-embedded biopsiesParaffin-embedded tissuesCD8 stainingLymphocyte phenotypingNonceliac patientsVillous bluntingDuodenal biopsiesRare conditionSpecialized centers
2018
CD68, CD163, and matrix metalloproteinase 9 (MMP-9) co-localization in breast tumor microenvironment predicts survival differently in ER-positive and -negative cancers
Pelekanou V, Villarroel-Espindola F, Schalper KA, Pusztai L, Rimm DL. CD68, CD163, and matrix metalloproteinase 9 (MMP-9) co-localization in breast tumor microenvironment predicts survival differently in ER-positive and -negative cancers. Breast Cancer Research 2018, 20: 154. PMID: 30558648, PMCID: PMC6298021, DOI: 10.1186/s13058-018-1076-x.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDAntigens, Differentiation, MyelomonocyticAntineoplastic AgentsBiomarkers, TumorBreastBreast NeoplasmsDisease-Free SurvivalFemaleGene Expression Regulation, NeoplasticHumansMacrophagesMatrix Metalloproteinase 9Middle AgedPatient SelectionPrognosisReceptors, Cell SurfaceReceptors, EstrogenRetrospective StudiesSurvival AnalysisTissue Array AnalysisTumor MicroenvironmentConceptsTumor-associated macrophagesOverall survivalQuantitative immunofluorescenceMacrophage markersBreast cancerHigh expressionPan-macrophage marker CD68Triple-negative breast cancerCD163/CD68Multiplexed quantitative immunofluorescenceImproved overall survivalProtein expressionWorse overall survivalPoor overall survivalMMP-9 protein expressionSubclass of patientsMacrophage-targeted therapiesMatrix metalloproteinase-9Tissue microarray formatMMP-9 proteinBreast tumor microenvironmentModulator of responseParaffin-embedded tissuesBreast cancer biomarkersCohort BMutation allele frequency threshold does not affect prognostic analysis using next-generation sequencing in oral squamous cell carcinoma
Ma J, Fu Y, Tu YY, Liu Y, Tan YR, Ju WT, Pickering CR, Myers JN, Zhang ZY, Zhong LP. Mutation allele frequency threshold does not affect prognostic analysis using next-generation sequencing in oral squamous cell carcinoma. BMC Cancer 2018, 18: 758. PMID: 30041611, PMCID: PMC6057048, DOI: 10.1186/s12885-018-4481-8.Peer-Reviewed Original ResearchConceptsOral squamous cell carcinomaSquamous cell carcinomaPrognostic analysisOSCC patientsCell carcinomaMethodsForty-six patientsClinical outcome analysisNext-generation sequencingAllele frequency thresholdWild-type genotypeParaffin-embedded tissuesNon-synonymous mutationsAllele frequenciesClinical outcomesOutcome analysisPatientsPanel of cancerType genotypeSignificant differencesCarcinomaFrequency thresholdNotch1CDKN2AMutationsCASP8Digital Polymerase Chain Reaction Quantification of SERPINA1 Predicts Prognosis in High-Grade Glioma
Ookawa S, Wanibuchi M, Kataoka-Sasaki Y, Sasaki M, Oka S, Ohtaki S, Noshiro S, Komatsu K, Akiyama Y, Mikami T, Mikuni N, Kocsis JD, Honmou O. Digital Polymerase Chain Reaction Quantification of SERPINA1 Predicts Prognosis in High-Grade Glioma. World Neurosurgery 2018, 111: e783-e789. PMID: 29309973, DOI: 10.1016/j.wneu.2017.12.166.Peer-Reviewed Original ResearchConceptsHigh-grade gliomasExpression of SERPINA1Polymerase chain reactionDigital polymerase chain reactionExpression groupMedian overall survivalAnti-inflammatory roleHigh expression groupMessenger RNA expressionCell linesGlioblastoma multiforme cell linesHuman glioblastoma cell linesParaffin-embedded tissuesPredict PrognosisOverall survivalPoor prognosisGrade IIIPolymerase chain reaction quantificationSERPINA1 expressionSurgical samplesGrade IVImmunohistochemical analysisGlioblastoma cell linesGlioma tissuesPrognosis
2017
Aberrant expression of epithelial leucine-rich repeat containing G protein–coupled receptor 5–positive cells in the eutopic endometrium in endometriosis and implications in deep-infiltrating endometriosis
Vallvé-Juanico J, Suárez-Salvador E, Castellví J, Ballesteros A, Taylor HS, Gil-Moreno A, Santamaria X. Aberrant expression of epithelial leucine-rich repeat containing G protein–coupled receptor 5–positive cells in the eutopic endometrium in endometriosis and implications in deep-infiltrating endometriosis. Fertility And Sterility 2017, 108: 858-867.e2. PMID: 28923287, DOI: 10.1016/j.fertnstert.2017.08.018.Peer-Reviewed Original ResearchConceptsG protein-coupled receptorsProtein-coupled receptorsEutopic endometriumEpithelial markersHealthy egg donorsHuman eutopic endometriumType of endometriosisEndometrium of womenMenstrual cycle phaseProspective experimental studyParaffin-embedded tissuesEndometriotic patientsInflammatory markersEndometrial samplesUterine aspiratesHuman endometriumLeucine-rich repeatsFluorescence-activated cell sortingPathologic endometriumMAIN OUTCOMEEndometriosisReproductive outcomesEndometriumFertility clinicsStromal compartment
2016
Unmasking of complements using proteinase-K in formalin fixed paraffin embedded renal biopsies
Nada R, Kumar A, Kumar V, Gupta K, Joshi K. Unmasking of complements using proteinase-K in formalin fixed paraffin embedded renal biopsies. Indian Journal Of Nephrology 2016, 26: 182-187. PMID: 27194832, PMCID: PMC4862263, DOI: 10.4103/0971-4065.159558.Peer-Reviewed Original ResearchRenal biopsyDirect immunofluorescenceMembranous glomerulonephritisImmune-complex mediated glomerulonephritisAutopsy casesImmune-Mediated InjuryCases of membranous glomerulonephritisImmunoglobulin A nephropathyAntigen retrievalComplement-mediated diseasesParaffin-embedded tissuesDense deposit diseaseMonoclonal diseaseRenal biopsy interpretationProteinase-KBiopsyFormalin-FixedAnti-GBMComplement stainingBiopsy interpretationImmune-complexesType 1Autopsy tissuesGlomerulonephritisFFPE tissues
2015
Loss of antigenicity with tissue age in breast cancer
Combs SE, Han G, Mani N, Beruti S, Nerenberg M, Rimm DL. Loss of antigenicity with tissue age in breast cancer. Laboratory Investigation 2015, 96: 264-269. PMID: 26568292, DOI: 10.1038/labinvest.2015.138.Peer-Reviewed Original ResearchConceptsHuman epidermal growth receptor 2Estrogen receptorQuantitative immunofluorescenceProtein expressionSeries of formalinRandom-effects modelHuman breast carcinomaLarge cooperative groupsParaffin-embedded tissuesKi67 expressionBreast carcinomaBreast cancerIndividual patientsTissue microarrayClinical investigationClinical questionsReceptor 2Tumor specimensPositive casesLoss of antigenicityFFPE biospecimensQuantitative protein expressionBiomarkersCooperative groupsPreservation time
2014
ERβ splice variant expression in four large cohorts of human breast cancer patient tumors
Wimberly H, Han G, Pinnaduwage D, Murphy LC, Yang XR, Andrulis IL, Sherman M, Figueroa J, Rimm DL. ERβ splice variant expression in four large cohorts of human breast cancer patient tumors. Breast Cancer Research And Treatment 2014, 146: 657-667. PMID: 25007965, PMCID: PMC6939385, DOI: 10.1007/s10549-014-3050-3.Peer-Reviewed Original ResearchConceptsBreast cancerPatient tumorsERβ variantsQuantitative immunofluorescenceBreast cancer patient tumorsTriple-negative patientsBreast cancer outcomesAbsence of ERαBreast cancer biologyCancer patient tumorsBreast cancer patient samplesSplice variant expressionCancer patient samplesParaffin-embedded tissuesQIF scoresCancer outcomesPredictive biomarkersWorse outcomesEstrogen receptorLarge cohortSurvival analysisERβPatient samplesAQUA technologyVariant expression
2013
In situ techniques for protein analysis in tumor tissue
Anagnostou V, Rimm D. In situ techniques for protein analysis in tumor tissue. 2013, 76-84. DOI: 10.1017/cbo9781139046947.010.Peer-Reviewed Original ResearchSignal amplification techniqueProtein detectionAntigen of interestConventional IHCAntigen-antibody reactionLow costHuman epidermal growth factor receptor 2Epidermal growth factor receptor 2Growth factor receptor 2Selection of patientsComplementary diagnostic informationSitu techniquesFactor receptor 2Companion diagnostic testsCurrent analytical techniquesParaffin-embedded tissuesHistological diagnosisSpecific therapyDefinite diagnosisHistological subclassificationBreast cancerPrognostic biomarkerEstrogen receptorReceptor 2Morphologic evaluation
2011
OT3-01-03: Pre-Surgical Evaluation of the AKT Inhibitor MK-2206 in Patients with Operable Invasive Breast Cancer: New York Cancer Consortium Trial P8740.
Kalinsky K, Sparano J, Kim M, Crew K, Maurer M, Taback B, Feldman S, Hibshoosh H, Wiechmann L, Adelson K, Hershman D. OT3-01-03: Pre-Surgical Evaluation of the AKT Inhibitor MK-2206 in Patients with Operable Invasive Breast Cancer: New York Cancer Consortium Trial P8740. Cancer Research 2011, 71: ot3-01-03-ot3-01-03. DOI: 10.1158/0008-5472.sabcs11-ot3-01-03.Peer-Reviewed Original ResearchPre-surgical studyInvasive breast cancerBreast cancerPI3K/AktWeekly MKPost-treatment tissue samplesOperable invasive breast cancerPeripheral blood mononuclear cellsEarly phase clinical studiesDownstream PI3K/Akt pathwayClinical stage IClinical trial programBlood mononuclear cellsMain eligibility criteriaPre-surgical evaluationEarly clinical testingPI3K/Akt pathwayEffects of MKAKT inhibitor MKPost-treatment samplesAnti-cancer propertiesParaffin-embedded tissuesAccrue patientsAnti-cancer agentsNeoadjuvant chemotherapyPD05-04: Quantitative Measurement of Antigen Degradation in NCCTG N9831 Tissue Microarrays.
Cheng H, Rimm D, Reinholz M, Lingle W, Ballman K, Dueck A, Chen B, McCullough A, Jenkins R, Perez E. PD05-04: Quantitative Measurement of Antigen Degradation in NCCTG N9831 Tissue Microarrays. Cancer Research 2011, 71: pd05-04-pd05-04. DOI: 10.1158/0008-5472.sabcs11-pd05-04.Peer-Reviewed Original ResearchCooperative group studiesTissue microarrayAntigen degradationAdjuvant phase III trialsPhase III trialsGroup studyExpression of HER2Paraffin-embedded tissuesIII trialsEntire cohortExpression of HER1Analysis of biomarkersPositive casesHER2Quantitative immunofluorescenceTumor samplesCancer ResHER2 scoreTissue blocksOld casesTissue collectionAverage scoreHER1TMA slidesPre-analytical variables
2010
Overexpression of EpCAM in Uterine Serous Papillary Carcinoma: Implications for EpCAM-Specific Immunotherapy With Human Monoclonal Antibody Adecatumumab (MT201)
El-Sahwi K, Bellone S, Cocco E, Casagrande F, Bellone M, Abu-Khalaf M, Buza N, Tavassoli FA, Hui P, Rüttinger D, Silasi DA, Azodi M, Schwartz PE, Rutherford TJ, Pecorelli S, Santin AD. Overexpression of EpCAM in Uterine Serous Papillary Carcinoma: Implications for EpCAM-Specific Immunotherapy With Human Monoclonal Antibody Adecatumumab (MT201). Molecular Cancer Therapeutics 2010, 9: 57-66. PMID: 20053761, PMCID: PMC2806489, DOI: 10.1158/1535-7163.mct-09-0675.Peer-Reviewed Original ResearchMeSH KeywordsAgedAntibodies, MonoclonalAntibodies, Monoclonal, HumanizedAntibody-Dependent Cell CytotoxicityAntigens, NeoplasmCarcinoma, PapillaryCell Adhesion MoleculesCell Line, TumorCell MembraneCystadenocarcinoma, SerousDrug Resistance, NeoplasmDrug Screening Assays, AntitumorEpithelial Cell Adhesion MoleculeFemaleFlow CytometryGene Expression Regulation, NeoplasticHumansImmunoglobulin GImmunohistochemistryImmunotherapyInterleukin-2Killer Cells, NaturalMiddle AgedNeoplasm MetastasisRNA, MessengerUterine NeoplasmsConceptsUterine serous papillary carcinomaUSPC cell linesNormal endometrial cellsPrimary USPC cell linesAntibody-dependent cellular cytotoxicitySerous papillary carcinomaCellular cytotoxicityPapillary carcinomaCell linesFlow cytometryAdvanced/recurrentStandard treatment modalityCell-dependent cytotoxicityUterine serous carcinomaComplement-dependent cytotoxicitySurface expressionHuman monoclonal antibodyNovel therapeutic strategiesFresh frozen biopsiesHigh surface expressionEpithelial cell adhesion moleculeOverexpression of EpCAMParaffin-embedded tissuesMedian copy numberSerous carcinoma
2008
Commercialized Multigene Predictors of Clinical Outcome for Breast Cancer
Ross JS, Hatzis C, Symmans WF, Pusztai L, Hortobágyi GN. Commercialized Multigene Predictors of Clinical Outcome for Breast Cancer. The Oncologist 2008, 13: 477-493. PMID: 18515733, DOI: 10.1634/theoncologist.2007-0248.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkers, TumorBreast NeoplasmsCost-Benefit AnalysisDevice ApprovalFemaleGene Expression ProfilingHumansMolecular Diagnostic TechniquesParaffin EmbeddingPredictive Value of TestsPrognosisReceptors, EstrogenReverse Transcriptase Polymerase Chain ReactionUnited StatesUnited States Food and Drug AdministrationConceptsQuantitative multiplex real-time polymerase chain reactionLymph node positiveValidation clinical trialEfficacy of tamoxifenER-positive patientsPredictive testRegulatory approval statusOncotype DX testCurrent clinical utilityReal-time polymerase chain reactionCytochrome P450 CYP2D6High accrual rateMultiplex real-time polymerase chain reactionParaffin-embedded tissuesThird-party payorsProspective trialYounger patientsNode positiveClinical outcomesOncotype DXPatient eligibilityMultigene assaysPolymerase chain reactionClinical trialsBreast cancerImmunohistochemical expression of folate receptor α in colorectal carcinoma: patterns and biological significance
Shia J, Klimstra D, Nitzkorski J, Low P, Gonen M, Landmann R, Weiser M, Franklin W, Prendergast F, Murphy L, Tang L, Temple L, Guillem J, Wong W, Paty P. Immunohistochemical expression of folate receptor α in colorectal carcinoma: patterns and biological significance. Human Pathology 2008, 39: 498-505. PMID: 18342661, DOI: 10.1016/j.humpath.2007.09.013.Peer-Reviewed Original ResearchConceptsFRalpha expressionColorectal carcinomaPrimary carcinomaMicrosatellite instability statusPositive stainingPresence of distant metastasesColorectal tissuesMetastatic colorectal carcinomaDisease-specific survivalInstability statusFolate receptor alphaTherapy of colorectal tumoursFeasibility of immunohistochemistryReverse transcriptase polymerase chain reactionTranscriptase polymerase chain reactionParaffin-embedded tissuesColorectal mucosa samplesDistant metastasisPrognostic significanceCancer therapy targetPrognostic valueImmunohistochemical expressionTissue microarrayReceptor ANormal mucosa
2007
Mammaglobin B expression in human endometrial cancer
TASSI RA, BIGNOTTI E, FALCHETTI M, CALZA S, RAVAGGI A, ROSSI E, MARTINELLI F, BANDIERA E, PECORELLI S, SANTIN AD. Mammaglobin B expression in human endometrial cancer. International Journal Of Gynecological Cancer 2007, 18: 1090-1096. PMID: 18021217, DOI: 10.1111/j.1525-1438.2007.01137.x.Peer-Reviewed Original ResearchConceptsReal-time polymerase chain reactionPolymerase chain reactionG3 casesNormal endometrial cellsHuman endometrial cancerFresh frozen biopsiesSignificant higher levelsParaffin-embedded tissuesEndometrial cancerG1 casesMammaglobin BNormal endometriumEndometrial tumorsEndometrial cellsOvarian cancerFrozen biopsyNovel molecular markersB expressionNEC controlsGene expression profilingImmunohistochemistryProtein expressionProtein levelsG2 casesChain reaction
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